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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="other" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">RUDN Journal of Agronomy and Animal Industries</journal-id><journal-title-group><journal-title xml:lang="en">RUDN Journal of Agronomy and Animal Industries</journal-title><trans-title-group xml:lang="ru"><trans-title>Вестник Российского университета дружбы народов. Серия: Агрономия и животноводство</trans-title></trans-title-group></journal-title-group><issn publication-format="print">2312-797X</issn><issn publication-format="electronic">2312-7988</issn><publisher><publisher-name xml:lang="en">Peoples’ Friendship University of Russia named after Patrice Lumumba</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">1708</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Articles</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Статьи</subject></subj-group><subj-group subj-group-type="article-type"><subject></subject></subj-group></article-categories><title-group><article-title xml:lang="en">Assessment of biotechnology capacity of various wheat varieties</article-title><trans-title-group xml:lang="ru"><trans-title>Оценка биотехнологического потенциала различных сортов пшеницы</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Mohamed</surname><given-names>S E</given-names></name><name xml:lang="ru"><surname>Мохамед</surname><given-names>С Е</given-names></name></name-alternatives><bio xml:lang="en">Кафедра ботаники, физиологии, патологии растений и агробиотехнологии; Российский университет дружбы народов; Russian People's Friendship University</bio><bio xml:lang="ru">Кафедра ботаники, физиологии, патологии растений и агробиотехнологии; Российский университет дружбы народов</bio><email>-</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Solovjova</surname><given-names>A I</given-names></name><name xml:lang="ru"><surname>Соловьева</surname><given-names>А И</given-names></name></name-alternatives><bio xml:lang="en">Институт физиологии растений им. К.А. Тимирязева РАН; Timirjazev Institute of Plant Physiology</bio><bio xml:lang="ru">Институт физиологии растений им. К.А. Тимирязева РАН</bio><email>-</email><xref ref-type="aff" rid="aff2"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Dolgikh</surname><given-names>Yu I</given-names></name><name xml:lang="ru"><surname>Долгих</surname><given-names>Ю И</given-names></name></name-alternatives><bio xml:lang="ru">Институт физиологии растений им. К.А. Тимирязева РАН</bio><email>-</email><xref ref-type="aff" rid="aff3"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">Russian People's Friendship University</institution></aff><aff><institution xml:lang="ru">Российский университет дружбы народов</institution></aff></aff-alternatives><aff-alternatives id="aff2"><aff><institution xml:lang="en">Timirjazev Institute of Plant Physiology</institution></aff><aff><institution xml:lang="ru">Институт физиологии растений им. К.А. Тимирязева РАН</institution></aff></aff-alternatives><aff-alternatives id="aff3"><aff><institution xml:lang="en"></institution></aff><aff><institution xml:lang="ru">Институт физиологии растений им. К.А. Тимирязева РАН</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2009-04-15" publication-format="electronic"><day>15</day><month>04</month><year>2009</year></pub-date><issue>4</issue><issue-title xml:lang="en">NO4 (2009)</issue-title><issue-title xml:lang="ru">№4 (2009)</issue-title><fpage>21</fpage><lpage>26</lpage><history><date date-type="received" iso-8601-date="2016-09-05"><day>05</day><month>09</month><year>2016</year></date></history><permissions><copyright-statement xml:lang="ru">Copyright ©; 2009, Мохамед С.Е., Соловьева А.И., Долгих Ю.И.</copyright-statement><copyright-year>2009</copyright-year><copyright-holder xml:lang="ru">Мохамед С.Е., Соловьева А.И., Долгих Ю.И.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">http://creativecommons.org/licenses/by/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://agrojournal.rudn.ru/agronomy/article/view/1708">https://agrojournal.rudn.ru/agronomy/article/view/1708</self-uri><abstract xml:lang="en">Assess the ability to embryogenic callus formation and regeneration of plants of several varieties of soft and hard spring wheat breeding Egyptian and Russian in order to select genotypes suitable for biotechnology. Callus from mature embryos were isolated from dry seeds and immature embryos isolated from kernels at 10-12 days after pollination. The highest frequency of embryogenic callus production from mature embryos marked for cultivar Novosibirskaya-22 (71,4%), the lowest capacity for embryogenesis has the cultivar Sids-1 (4%). For some genotypes the higher frequency of production embryogenic callus obtained in the use of mature, but for others - immature embryos. For one genotypes higher frequency of embryogenic callus formation is received at use mature, and for others - immature embryos. With increase in age of culture the proportion of embryogenic calli was reduced at all cultivars, after four months of culturing it became close to zero. Novosibirskaya-22, Suhadzh-1, Gemmiza-1 can be used as sites of cell selection or genetic engineering.</abstract><trans-abstract xml:lang="ru">С целью отбора генотипов, пригодных для биотехнологии, оценена способность ряда сортов мягкой и твердой яровой пшеницы египетской и российской селекции к образованию эмбриогенного каллуса и регенерации растений. Каллус получали из зрелых зародышей, выделенных из сухих зерновок, и из незрелых зародышей, изолированных на 10-12-й день после опыления. Наибольшая частота образования эмбриогенного каллуса отмечена для сорта Новосибирская-22 (71,4%), наименьшей способностью к эмбриогенезу обладал сорт Сидс-1 (4%). Для одних генотипов более высокая частота образования эмбриогенного каллуса получена при использовании зрелых, а для других - незрелых зародышей. С увеличением возраста культуры доля эмбриогенных каллусов сокращалась у всех сортов, после четырех месяцев культивирования она становилась близкой к нулю. Новосибирская-22, Сухадж-1, Геммиза-1 могут быть использованы в качестве объектов клеточной селекции или генетической инженерии.</trans-abstract><kwd-group xml:lang="en"><kwd>soft wheat</kwd><kwd>hard wheat</kwd><kwd>callus</kwd><kwd>embryo</kwd><kwd>embryogenic callus</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>пшеница мягкая</kwd><kwd>пшеница твердая</kwd><kwd>каллус</kwd><kwd>зародыш</kwd><kwd>эмбриогенный каллус</kwd></kwd-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Redway F.A., Vasil V., Lu D., Vasil I.K. Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.) // Theor. Appl. Genet. - 1990. - V. 79. - P. 609-617.</mixed-citation></ref><ref id="B2"><label>2.</label><mixed-citation>Chawla H.S. Regeneration responses of callus from different explants and changes on isozymes during morphogenesis in wheat // Biol. Plantarum. - 1989. - V. 31. - P. 121-125.</mixed-citation></ref><ref id="B3"><label>3.</label><mixed-citation>Chowdhury S.H., Kato K., Yamamoto Y., Hayashi K. Varietal variation in plant regeneration capacity from immature embryos among common wheat cultivars // Japan J. Breed. - 1991. - V. 41. - P. 443-450.</mixed-citation></ref><ref id="B4"><label>4.</label><mixed-citation>Ozgen M., Turet M., Ozcan S., Sancak C. Callus induction and Plant regeneration from immature and mature embryos of winter durum wheat genotypes // Plant Breed. - 1996. - V. 115. - P. 455-458.</mixed-citation></ref><ref id="B5"><label>5.</label><mixed-citation>Bohorova N.E., Pfeiffer W.H., Mergoum M., Crossa J., Pacheco M., Estanol P. Regeneration potential of CIMMYT durum wheat and triticale vatieties from immature embryos // Plant Breed. - 2001. - V. 120. - P. 291-295.</mixed-citation></ref><ref id="B6"><label>6.</label><mixed-citation>Haliloglu K. Wheat immature embryo culture for embryogenic callus induction // J. Biol. Sci. - 2002. - V. 2. - P. 520-521.</mixed-citation></ref><ref id="B7"><label>7.</label><mixed-citation>Ежова Т.А., Багрова А.М., Хартина Г.А., Гостимский С.А. Цитогенетический анализ длительно культивируемых каллусов и полученных из них регенерантов гороха (Pisum sativum L.) // Цитология и генетика. - 1988. - Т. 22. - С. 22-26.</mixed-citation></ref><ref id="B8"><label>8.</label><mixed-citation>Duncan D.R., Waskot R.M., Nabors M.W. In vitro screening and field evaluation of tissue-culture-regenerated sorghum (Sorghum bicolor (L.) Moench) for soil stress tolerance // Euphytica. - 1995. - V. 85. - P. 373-380.</mixed-citation></ref><ref id="B9"><label>9.</label><mixed-citation>Zale J.V., Borchardt-Wier H., Kidwell K.K., Steber C.V. Callus induction and plant regeneration from mature embryos of a diverse set of wheat genotypes // Plant Cell, Tissue and Organ Culture. - 2004. - V. 76. - P. 277-281.</mixed-citation></ref><ref id="B10"><label>10.</label><mixed-citation>Yu Y., Wang J., Zhu M.L., Wey Z.M. Optimization of mature embryos based high frequency callus induction and plant regeneration from elite wheat cultivars grown in China // Plant Breeding. - 2008. - V. 127. - P. 249-255.</mixed-citation></ref></ref-list></back></article>
