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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">RUDN Journal of Agronomy and Animal Industries</journal-id><journal-title-group><journal-title xml:lang="en">RUDN Journal of Agronomy and Animal Industries</journal-title><trans-title-group xml:lang="ru"><trans-title>Вестник Российского университета дружбы народов. Серия: Агрономия и животноводство</trans-title></trans-title-group></journal-title-group><issn publication-format="print">2312-797X</issn><issn publication-format="electronic">2312-7988</issn><publisher><publisher-name xml:lang="en">Peoples’ Friendship University of Russia named after Patrice Lumumba</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">1734</article-id><article-id pub-id-type="doi">10.22363/2312-797X-2015-3-56-68</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Articles</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Статьи</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Contribution of consensus 5'-untranslated region to the translational efficiency of heterologous genes in plant cells</article-title><trans-title-group xml:lang="ru"><trans-title>Вклад консенсусной 5’-нетранслируемой области в эффективность трансляции гетерологичных генов в растительных клетках</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Quimisse</surname><given-names>M G</given-names></name><name xml:lang="ru"><surname>Кимиссе</surname><given-names>Мариу Гилерме</given-names></name></name-alternatives><bio xml:lang="en">Department of botany, plant physiology and agrobiotechnology</bio><bio xml:lang="ru"><p>Кафедра ботаники, физиологии растений и агробиотехнологии</p></bio><email>kimisse@rambler.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Kabardaeva</surname><given-names>K V</given-names></name><name xml:lang="ru"><surname>Кабардаева</surname><given-names>Ксения Владимировна</given-names></name></name-alternatives><email>kabardaewa@yandex.ru</email><xref ref-type="aff" rid="aff2"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Gra</surname><given-names>O A</given-names></name><name xml:lang="ru"><surname>Гра</surname><given-names>Ольга Алексеевна</given-names></name></name-alternatives><email>olgagra@gmail.com</email><xref ref-type="aff" rid="aff3"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Tyurin</surname><given-names>A A</given-names></name><name xml:lang="ru"><surname>Тюрин</surname><given-names>Александр Александрович</given-names></name></name-alternatives><email>alexjofar@gmail.com</email><xref ref-type="aff" rid="aff3"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">Peoples’ Friendship University of Russia</institution></aff><aff><institution xml:lang="ru">Российский университет дружбы народов</institution></aff></aff-alternatives><aff-alternatives id="aff2"><aff><institution xml:lang="en">RSAU - MTAA named after K.A. Timiryazev</institution></aff><aff><institution xml:lang="ru">РГАУ - МСХА имени К.А. Тимирязева</institution></aff></aff-alternatives><aff-alternatives id="aff3"><aff><institution xml:lang="en">FSBIS K.A. Timiryazev institute of plant physiology RAS</institution></aff><aff><institution xml:lang="ru">ФГБУН Институт физиологии растений им. К.А. Тимирязева РАН</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2015-03-15" publication-format="electronic"><day>15</day><month>03</month><year>2015</year></pub-date><issue>3</issue><issue-title xml:lang="en">NO3 (2015)</issue-title><issue-title xml:lang="ru">№3 (2015)</issue-title><fpage>56</fpage><lpage>68</lpage><history><date date-type="received" iso-8601-date="2016-09-05"><day>05</day><month>09</month><year>2016</year></date></history><permissions><copyright-statement xml:lang="ru">Copyright ©; 2015, Кимиссе М.Г., Кабардаева К.В., Гра О.А., Тюрин А.А.</copyright-statement><copyright-year>2015</copyright-year><copyright-holder xml:lang="ru">Кимиссе М.Г., Кабардаева К.В., Гра О.А., Тюрин А.А.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">http://creativecommons.org/licenses/by/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://agrojournal.rudn.ru/agronomy/article/view/1734">https://agrojournal.rudn.ru/agronomy/article/view/1734</self-uri><abstract xml:lang="en">The results of bioinformatics analysis allowed to reveal the following consistent pattern: the average length of the 5'-untranslated region (5'-UTRs) for most A. thaliana's genes with high expression levels range from 80 to 120 bp, with an average GC content of 36,5%. Based on alignment results was defined motive in 5'-UTRs, as a new regulatory element, which could potentially provide highly efficient expression and synthesis of the target product in plants. This sequence has a length of 87 bp and GC content of 35.6%. It was demonstrated that consensus sequence of 5'-UTRs increased accumulation of the bi-reporter protein more than 25%, by that acting as a potential positive regulatory element on translational efficiency.</abstract><trans-abstract xml:lang="ru"><p>Результаты биоинформатического анализа позволили выявить следующую закономерность: средний размер 5’-нетранслируемой области (5'-НТО) для большинства генов A. thaliana с высоким уровнем экспрессии варьирует от 80 до 120 п. н. со средним содержанием GC 36,5%. На основании результатов выравнивания определена консенсусная 5'-НТО как новый регуляторный элемент, который потенциально может обеспечить высокоэффективную экспрессию и синтез целевого продукта в растениях. Эта последовательность имеет размер 87 п.н. и GC содержание 35,6%. Продемонстрировано, что консенсусная 5'-НТО обеспечивает увеличение накопления бирепортерного белка более, чем на 25%, тем самым выступает в качестве потенциального позитивного регуляторного элемента в эффективности трансляции.</p></trans-abstract><kwd-group xml:lang="en"><kwd>plants</kwd><kwd>5'-untranslated region</kwd><kwd>translation</kwd><kwd>gene expression</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>растения</kwd><kwd>5’-нетранслируемая область</kwd><kwd>трансляция</kwd><kwd>экспрессия генов</kwd></kwd-group><funding-group/></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Вячеславова А.О. Серия модульных векторов для стабильной и транзиентной экспрессии гетерологичных генов в растениях / А.О. Вячеславова, О.Н. Мустафаев, А.А. Тюрин, Х.Р. Шимшилашвили, И.Н. Бердичевец, Д.М. Шаяхметова, М.А. Голденков, В.С. Фадеев, Ю.В. Шелудько, И.В. Голденкова-Павлова // Генетика. 2012. Т. 48. № 9. С. 1046-1056.</mixed-citation></ref><ref id="B2"><label>2.</label><mixed-citation>Маниатис Т., Фрич Э., Сэмбрук Д. Молекулярное клонирование. М.: Мир, 1984.</mixed-citation></ref><ref id="B3"><label>3.</label><mixed-citation>Bradford M.A. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding // Analytical Biochemistry. 1976. Vol. 72. P. 248-254.</mixed-citation></ref><ref id="B4"><label>4.</label><mixed-citation>Calvo S.E., Pagliarini D.J., Mootha V.K. Upstream open reading frames cause wide spread reduction of protein expression and are polymorphic among humans // Proceedings of the National Academy of Sciences of the United States of America. 2009. Vol. 106. P. 7507-7512.</mixed-citation></ref><ref id="B5"><label>5.</label><mixed-citation>Iacono M., Mignone F., Pesole G. uAUG and uORFs in human and rodent 5′untranslated mRNAs // Gene. 2005. Vol. 349. P. 97-105.</mixed-citation></ref><ref id="B6"><label>6.</label><mixed-citation>Kozak M. Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes // Proceedings of the National Academy of Sciences of the United States of America. 1990. Vol. 87. P. 8301-8305.</mixed-citation></ref><ref id="B7"><label>7.</label><mixed-citation>Kozak M. Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo // Nature. 1984. Vol. 308. P. 241-246.</mixed-citation></ref><ref id="B8"><label>8.</label><mixed-citation>Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translatin by eukaryotic ribosomes // Cell. 1986. Vol. 44. P. 283-292.</mixed-citation></ref><ref id="B9"><label>9.</label><mixed-citation>Mann D.G.J., King Z.R., Liu W., Joyce B.L., Percifield R.J., Hawkins J.S., LaFayette P.R., Artelt B.J., Burris J.N., Mazarei M., Bennetzen J.L., Parrott W.A., Neal Stewart C.J. Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation // BMC Biotechnol. 2011. Vol. 11. № 11. P. 74.</mixed-citation></ref><ref id="B10"><label>10.</label><mixed-citation>Masura S.S., Ahmad P.G.K., Ti L.L.E. Isolation and characterization of an oil palm constitutive promoter derived from a translationally control tumor protein (TCTP) gene // Plant Physiology and Biochemistry. 2011. Vol. 49. P. 701-708.</mixed-citation></ref><ref id="B11"><label>11.</label><mixed-citation>Park S.H., Yi N., Kim Y.S., Jeong M.H., Bang S. W, Choi Y.D., Kim J.K. Analysis of five novel putative constitutive gene promoters in transgenic rice plants // Journal of Experimental Botany. 2010. Vol. 61. № 9. P. 2459-2467.</mixed-citation></ref><ref id="B12"><label>12.</label><mixed-citation>Pelletier J., Sonenberg N.J. Insertion mutagenesis to increase secondary structure within the 5′ noncoding region of a eukaryotic mRNA reduces translational efficiency // Cell. 1985. Vol. 40. P. 515-526.</mixed-citation></ref><ref id="B13"><label>13.</label><mixed-citation>Wood T.M., Bhat K.M. Methods for measuring cellulase activities // Methods in Enzymology. 1988. Vol. 160. P. 87-112.</mixed-citation></ref></ref-list></back></article>
