RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal IndustriesВестник Российского университета дружбы народов. Серия: Агрономия и животноводство2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1942610.22363/2312-797X-2018-13-4-287-293Veterinary sanitary inspectionВетеринарно-санитарная экспертизаResearch ArticlePREGNANCY-ASSOCIATED GLYCOPROTEIN CONCENTRATIONS IN NON-PREGNANT COWS: A CASE STUDYСТЕЛЬНОСТЬ-АССОЦИИРОВАННЫЕ КОНЦЕНТРАЦИИ ГЛИКОПРОТЕИНА У НЕСТЕЛЬНЫХ КОРОВAyadAbdelhanineAyadAProfessor (DVM, MSc, PhD), Department of Biological Sciences of Environment, Faculty of Life and Nature Sciences, University of Bejaia.hanine06@gmail.comTouatiKamalTouatiKSurgery for Large Animals, Faculty of Veterinary Medicine, University of Liege, Belgiumhanine06@gmail.comUniversity of BejaiaUniversity of Liege15122018134VOL 13, NO4 (2018)ТОМ 13, №4 (2018)28729328122018Copyright ©; 2018, Ayad A., Touati K.Copyright ©; 2018, Ayad A., Touati K.2018Ayad A., Touati K.Ayad A., Touati K.http://creativecommons.org/licenses/by/4.0https://agrojournal.rudn.ru/agronomy/article/view/19426The aim of the present work was undertaken to describe three cases of the bPAG concentration measure by three RIA systems during the post-partum period in non-pregnantcow. Three Holstein-Friesian cows of mixed age and parity were diagnosed as non-pregnant. Blood samples were removed from the coccygeal vessel into EDTA-coated tubes. Samples were collected every 2 days during a stabling period of two months in the absence of males. Plasma was obtained by centrifugation immediately after collection and was stored at -20 C until assay. Plasmatic PAG concentration was measured by radioim-munoassay technique with some modifications. In RIA-780 and RIA-809, there were high peak of PAG concentration that reached 2.56 ng/ml and 0.89 ng/ml, respectively. These peaks lasted longer than 3 days (two successive samples were positive). The other RIA systems gave the values of PAG concentrations below cut-off of pregnancy diagnosis (> 0.8 ng/ml) and remains always non-pregnant. Our data shows clearly that there is another source of glycoproteins expression (e.g. ovarian) apart from the placenta in cow. The present study could be a field for future larger studies on the same subject by exploring deeply other extra structures placental.Целью настоящей работы было сравнительное описание трех вариантов измерения концентрации bPAGс использованием трех систем RIA в послеродовом периоде у нестельных коров. Три коровы голштинско-фризской породы смешанного возраста были диагностированы как нестельные. Образцы крови отбирались из хвостовой артерии в пробирки, c покрытием EDTA. Образцы собирали каждые 2 дня в 2-месячный стойловый период в отсутствии быков. Плазму получали центрифугированием сразу после сбора и хранили до начала анализа при температуре -20 C. Концентрацию PAG в плазме измеряли с помощью радиоиммунологического анализа с некоторыми изменениями. В RIA-780 и RIA-809 наблюдался высокий пик концентрации PAG, достигающий 2,56 нг/мл и 0,89 нг/мл соответственно. Эти пики продолжались более 3 дней (два последовательных образца были положительными). Другие системы RIA показывали концентрацию PAG ниже минимальной границы для периода стельности (> 0,8 нг/мл) и всегда оставались такими. Наши данные ясно показывают, что есть другой источник экспрессии гликопротеинов (например, яичники), кроме плаценты у коровы. Настоящее исследование могло бы стать предметом для будущих более масштабных исследований по этому же вопросу, основываясь на более глубоком изучении других структурных элементов плаценты.PAGradioimmunoassaynon-pregnantcowПАГрадиоиммунологический анализнестельнаякорова1.Green JA, Xie S, Quan X, Bao B, Gan X, Mathialagan N, et al. Pregnancy-associated bovine and ovine glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biology of reproduction. 2000; 62(6):1624-1631.2.Zoli AP, Demez P, Beckers JF, Reznik M, Beckers A. Light and electron microscopic immuno- localization of bovine pregnancy-associated glycoprotein in the bovine placentome. Biology of Reproduction. 1992; 46(4):623-629.3.Guruprasad K, Blundell TL, Xie S, Green J, Szafranska B, Nagel RJ, et al. Comparative modelling and analysis of amino acid substitutions suggests that the family of pregnancy- associated glycoproteins includes both active and inactive aspartic proteinases. Protein Engineering, Design and Selection. 1996; 9(10):849-856.4.Xie S, Low BG, Nagel RJ, Kramer KK, Anthony RV, Zoli AP, et al. Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. Proceedings of the National Academy of Sciences. 1991; 88(22):10247-10251.5.Xie S, Green J, Bao B, Beckers JF, Valdez KE, Hakami L, et al. Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine placental tissue. Biology of Reproduction. 1997; 57(6):1384-1393.6.Hughes AL, Green JA, Garbayo JM, Roberts RM. Adaptive diversification within a large family of recently duplicated, placentally expressed genes. Proceedings of the National Academy of Sciences. 2000; 97(7):3319-3323.7.Szenci O, Beckers J-F, Humblot P, Sulon J, Sasser G, Taverne M, et al. Comparison of ultra- sonography, bovine pregnancy-specific protein B, and bovine pregnancy-associated glyco- protein 1 tests for pregnancy detection in dairy cows. Theriogenology. 1998; 50(1):77-88.8.Humblot P, Camous S, Martal J, Charlery J, Jeanguyot N, Thibier M, et al. Diagnosis of pregnancy by radioimmunoassay of a pregnancy-specific protein in the plasma of dairy cows. Theriogenology. 1988; 30(2):257-267.9.Garth Sasser R, Ruder CA, Ivani KA, Butler JE, Hamilton WC. Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation. Biology of Reproduction. 1986; 35(4):936-942.10.PagnahZoli A, Beckers J-F, Wouters-Ballman P, Closset J, Falmagne P, Ectors F. Purification and characterization of a bovine pregnancy-associated glycoprotein. Biology of Reproduction. 1991;45(1):1-10.11.Greenwood FC, Hunter WM, Glover J. The preparation of 131I-labelled human growth hormone of high specific radioactivity. Biochemical journal. 1963;89(1):114.12.Vaitukaitis J, Robbins J, Nieschlag E, Ross G. A method for producing specific antisera with small doses of immunogen. The Journal of Clinical Endocrinology & Metabolism. 1971; 33(6):988-991.13.Zoli AP, Guilbault LA, Delahaut P, Ortiz WB, Beckers JF. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biology of Reproduction. 1992; 46(1):83-92.14.El Amiri B, Remy B, Sousa NM, Joris B, Ottiers NG, Perenyi Z, et al. Isolation and partial characterization of three pregnancy associated glycoproteins from the ewe placenta. Molecular Reproduction and Development: Incorporating Gamete Research. 2003; 64(2):199-206.15.El Amiri B, Remy B, De Sousa NM, Beckers JF. Isolation and characterization of eight preg- nancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation. Reproduction Nutrition Development. 2004; 44(3):169-181.16.Ayad A, Sousa N, Sulon J, Iguer-Ouada M, Beckers JF. Comparison of five radioimmunoassay systems for PAG measurement: ability to detect early pregnancy in cows. Reproduction in domestic animals. 2007; 42(4):433-440.17.Chard T. Pregnancy protein in the human. Biological and clinical significance. In: Hau J. (ed.) Pregnancy proteins in animals. Berlin: Walter de Gruyter; 1986. p. 9-20.18.Tatarinov YS, Masyukevich VN. Immunochemical identified of new beta-l-globulin in blood serum of pregnant women. Bull. Eksp. Biol. Med. 1970; 69: 66-68. (In Russ).19.Butler J, Hamilton W, Sasser R, Ruder C, Hass G, Williams R. Detection and partial characteriza- tion of two bovine pregnancy-specific proteins. Biology of Reproduction. 1982; 26(5):925-33.20.Garbayo JM, Remy B, Alabart JL, Folch J, Wattiez R, Falmagne P, et al. Isolation and partial characterization of a pregnancy-associated glycoprotein family from the goat placenta. Biology of reproduction. 1998; 58(1):109-15.