RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal IndustriesВестник Российского университета дружбы народов. Серия: Агрономия и животноводство2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1943710.22363/2312-797X-2018-13-4-396-404Agricultural economyЭкономика и развитие АПК (агропромышленного комплекса)Research ArticleTHE INTEREST OF HYPEROSMOLAR EXTENDERS IN ROOSTER SPERM CRYOPRESERVATIONГИПЕРПОСМОЛЯРНЫЕ НАПОЛНИТЕЛИ СПЕРМЫ ПЕТУХОВ ПРИ ЕЕ КРИОКОНСЕРВАЦИИIguer-ouadaMokrane MIguerouadaMokrane Mimokrane@gmail.comNorezzineAbdelazizassissnor@gmail.comRebouhNazih Yacern.rebouh@outlook.frUniversity of BejaiaErnst Institute for Animal HusbandryNemchinovka Moscow Research Institute of Agriculture15122018134VOL 13, NO4 (2018)ТОМ 13, №4 (2018)39640428122018Copyright ©; 2018, Iguer-ouada M.M., Norezzine A., Rebouh N.Y.Copyright ©; 2018, Iguerouada M.M., Norezzine A., Rebouh N.Y.2018Iguer-ouada M.M., Norezzine A., Rebouh N.Y.Iguerouada M.M., Norezzine A., Rebouh N.Y.http://creativecommons.org/licenses/by/4.0https://agrojournal.rudn.ru/agronomy/article/view/19437The aim of the present study was to investigate thepotential benefit of hyperosmolar extender during the freezing-thawing process in rooster sperm. The goal was to minimize the amount of intracellular water and reduce the impact of intracellular icecrystal during the freezing process. A total of five 45-week old Hubbard commercial broilers were subjected to bi-weekly semen collections. Collected sperm was pooled and divided in three aliquots. The control aliquot was diluted with Tris-extender without further supplementation at 300 mOsm (Control). The two (2) other aliquots were diluted with Trisextender at 300 mOsm containing vitamin E (Vit E) or with Tris-extender without Vitamin E but at osmolarity of 450 mOsm (Hyper). After incubation at 22 °C for 15 min, all aliquots were cooled and then frozen in liquid nitrogen. A Computer Aided Semen Analysis (CASA) was used to investigate the impact on different motility parameters. After thawing, Hyperosmolar (Hyper) and vitamin E extenders showed the highest values in terms of sperm motility preservation. Hyperosmolar extender (Hyper) showed particularly the highest values in terms of VSL velocities and progressive motile spermatozoa, known as indicators of sperm quality. In conclusion, the present results revealed that a significant impact was observed when using extenders at osmolarity of 450 mOsmin rooster sperm, in the same manner and even better then when using vitamin E. The positive impact is probably related to the reduction of intracellular ice formation.Целью настоящей работы было исследование потенциального преимущества гиперосмолярного наполнителя в процессе замораживания-оттаивания спермы петухов. Цель заключалась в том, чтобы свести к минимуму количество внутриклеточной воды и уменьшить влияние внутриклеточного льда в процессе замораживания. В общей сложности у пяти 45-недельных коммерческих бройлеров кросса «Hubbard» были взяты образцы спермы в течение двухнедельного периода. Собранную сперму объединяли и делили на три равные части. Контрольный образец разбавляли трис-наполнителем без дополнительных добавок при 300 mOsm (контроль). Два других образца разбавляли трис-наполнителем при 300 мОм, содержащим витамин Е (Vit E), или с трис-наполнителем без витамина Е, но при осмолярности 450 mOsm (Hyper). После инкубации при 22 °С в течение 15 мин все образцы охлаждали и затем замораживали в жидком азоте. Для исследования влияния на различные параметры моторики был использован анализ спермы с использованием метода (CASA). После оттаивания наполнители с Hyperosmolar (Hyper) и витамином Е показали наилучшие значения с точки зрения сохранения подвижности сперматозоидов. Гиперосмолярный наполнитель (Hyper) показал особенно высокие значения в отношении скорости движения VSL и прогрессирующих подвижных сперматозоидов, известных как показатели качества спермы. Представленные результаты показали, что при использовании наполнителей спермы петухов при осмолярности, равной 450 mOsm, наблюдали существенное улучшение показателей сперматозоидов, которое было даже лучше, чем при использовании витамина Е. Положительное влияние, вероятно, связано с уменьшением внутриклеточного образования льда.Poultryspermcryopreservationhyperosmolar extenderoxidative stresscold chockspermatozoaintracellular waterптицаспермакриоконсервациягиперосмолярный наполнительокислительный стрессхолодный подноссперматозоидывнутриклеточная вода1.Benhenia K, Lamara A, Fatmi S, Iguer-Ouada M. Effect of cyclodextrins, cholesterol and vitamin E and their complexation on cryopreserved epididymal ram semen. Small Ruminant Research. 2016; 141:29-35.2.Slima AB, Ali MB, Barkallah M, Traore AI, Boudawara T, Allouche N, Gdoura R. Antioxidant properties of Pelargonium graveolens L’Her essential oil on the reproductive damage induced by deltamethrin in mice as compared to alpha-tocopherol. 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