RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal IndustriesВестник Российского университета дружбы народов. Серия: Агрономия и животноводство2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1947010.22363/2312-797X-2019-14-1-7-17Crop productionРастениеводствоResearch ArticleEFFECT OF BETA-AMINOBUTYRIC ACID ON ACTIVITY OF PEROXIDASE AND POLYPHENOL OXIDASE ENZYMES IN CUCUMBER INFECTED WITH NEMATODE MELOIDOGYNE JAVANICAВЛИЯНИЕ БЕТА-АМИНОМАСЛЯНОЙ КИСЛОТЫ НА АКТИВНОСТЬ ПЕРОКСИДАЗЫ И ПОЛИФЕНОЛ-ОКСИДАЗЫ У РАСТЕНИЙ ОГУРЦА, ПОРАЖЕННЫХ НЕМАТОДОЙ MELOIDOGYNE JAVANICAMahmoudiNiloufar

Department of AgroBiotechnology, Agrarian and Technological Institute

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niloofarmahmoodi@ymail.comPeoples’ Friendship University of Russia (RUDN University)Российский университет дружбы народов15122019141VOL 14, NO1 (2019)ТОМ 14, №1 (2019)71711042019Copyright ©; 2019, Mahmoudi N.Copyright ©; 2019, Mahmoudi N.2019Mahmoudi N.Mahmoudi N.http://creativecommons.org/licenses/by/4.0https://agrojournal.rudn.ru/agronomy/article/view/19470

Beta-amino-butyric acid is introduced as an inducer of resistance in plants against plant pathogens. In this research, the induction of some defensive compounds including peroxidase and polyphenol oxidase enzymes by this compound against the rootstock node-producing nematode Meloidogyne javanica in cucumber was investigated. The results showed that inoculation of cucumber roots infected with root-knot nematode with the chemical composition of BABA from the first day after inoculation increased the activity of peroxidase enzyme and reached its maximum on the fourth day of this activity. Induction of the activity of polyphenol oxidase enzyme showed a gradual increase with a significant difference in comparison to the control and healthy plants and reached the maximum on the fourth day. The electrophoresis of peroxidase isozymes showed that isozyme peroxidase forms in cucumber roots induced by BABA were much more potent than pathogen-induced isozymes. In plants treated with nematode plus BABA, two isozymes were Rf = 00.31 and Rf = 34/0, which were stronger than control (inoculated with nematode).

Бета-аминомасляная кислота вводится в качестве индуктора устойчивости растений к патогенам растений. В данной работе была исследована индукция некоторых защитных соединений, в том числе ферментов пероксидазы и полифенолоксидазы этим соединением, против нематоды Meloidogyne javanica , продуцирующей узел корневища, в огурце. Результаты показали, что инокуляция корней зараженного огурца нематодой из корневых узлов с химическим составом БAМК с первого дня после инокуляции нематодой увеличивала активность фермента пероксидазы и достигала своего максимума на четвертый день этой активности. Индукция активности фермента полифенолоксидазы показала постепенное увеличение со значительной разницей по сравнению с контрольными и здоровыми растениями и достигла максимума на четвертый день. Электрофорез пероксидазных изозимов показал, что изоферментные пероксидазные формы в корнях огурца, индуцированные БAМК, гораздо более эффективны, чем индуцируемые патогеном изозимы. У растений, обработанных нематодой плюс БAМК, два изофермента имеют Rf = 00,31 и Rf = 34/0, которые являются более сильными, чем контроль (инокулированные нематодой).

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