RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal IndustriesВестник Российского университета дружбы народов. Серия: Агрономия и животноводство2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1951210.22363/2312-797X-2019-14-3-266-278Veterinary scienceВетеринарияResearch ArticleGalactooligosaccharide effects as prebiotic on intestinal microbiota of different fish speciesВлияние галактоолигосахаридов в виде пребиотика на микрофлору кишечника различных видов рыбHoseinifarSeyed HosseinХосейнифарСейед Хоссейн

Phd, Department of Life and Environmental Sciences

факультет наук о жизни и окружающей среде

hossein.hoseinifar@gmail.comDoanHien VanДоанХиен Ван

Phd, Department of Animal and Aquatic Sciences, Faculty of Agriculture

факультет наук о животных и водных животных, сельскохозяйственный факультет

hossein.hoseinifar@gmail.comAshouriGhasemАшуриГасем

Department of Life and Environmental Sciences

факультет наук о жизни и окружающей среде

hossein.hoseinifar@gmail.comPolytechnic University of MarcheПолитехнический Университет МаркеChiang Mai UniversityЧиангмайский университет15122019143VOL 14, NO3 (2019)ТОМ 14, №3 (2019)26627803102019Copyright ©; 2019, Hoseinifar S.H., Doan H.V., Ashouri G.Copyright ©; 2019, Хосейнифар С.Х., Доан Х.В., Ашури Г.2019Hoseinifar S.H., Doan H.V., Ashouri G.Хосейнифар С.Х., Доан Х.В., Ашури Г.http://creativecommons.org/licenses/by/4.0https://agrojournal.rudn.ru/agronomy/article/view/19512

Manipulation of the gut microbiota toward potentially beneficial bacteria (probiotics) has beneficial effects on fish physiology and health. The effects of prebiotics on gut microbiota are species specific. The present study aimed at investigation of the effects of galactooligosaccharide (GOS) as prebiotic on intestinal microbiota of Caspian roach and Caspian white fish fingerlings. which are among the most economically valuable species in the Caspian Sea. The study was conducted in a completely randomized design with two set of experiment each of them include three treatments in triplicates in which 0 (control), 1 and 2% GOS were used in diet for 6 weeks. At the end of the period, changes in the intestinal microbiota, including total bacterial count, lactic acid count and lactic acid bacteria (LAB) levels and dominance of LAB in the intestinal microbiota, were measured by culture-based method. Dietary GOS had no significant effect on total bacterial count in both species (P < 0.05). The LAB levels in the intestinal microbiota in the treatments fed with prebiotics was significantly higher than the control group (P < 0.05). LAB bacteria showed the highest increase and dominance in treatments fed with 2% GOS. Also, the highest ratio of lactic acid bacteria to the total number of viable bacteria was observed in the treatment with 2% GOS treatment (P < 0.05). The results of this study indicated the possibility of alterations in the bacterial communities of Caspian roach and Caspian white fish fingerlings gut toward beneficial bacterial communities using GOS as prebiotic.

Обогащение кишечной микробиоты потенциально полезными бактериями (пробиотиками) оказывает благотворное влияние на физиологические процессы и здоровье рыб. Однако, воздействие пребиотиков на микрофлору кишечника является видоспецифичным. Настоящее исследование направлено на изучение влияния галактоолигосахаридов в качестве пребиотика на кишечную микробиоту каспийской плотвы и мальков каспийского кутума, являющихся одними из наиболее экономически ценных видов рыб, обитающих в Каспийском море. Исследование проводилось в течение 6 недель по полной рандомизированной схеме, в двух повторениях, каждое из которых включало три варианта обработки - 0 (контроль), 1 и 2 % ГОС, в трехкратной повторности. После этого с помощью культурального метода были изучены изменения в микробиоте кишечника рыб, включая общее количество бактерий, количество молочной кислоты и молочнокислых бактерий, а также влияние молочнокислых бактерий на микрофлору кишечника. Диетические галактоолигосахариды не оказали значительного влияния на общее количество бактерий у обоих видов ( P < 0.05). Уровень молочнокислых бактерий в кишечнике был значительно выше при лечении пребиотиками, чем в контрольной группе ( P < 0.05). Значительное увеличение количества молочнокислых бактерий и их преобладание было отмечено в варианте с использованием 2 % галактоолигосахаридов. Кроме того, самое высокое количество молочнокислых бактерий по отношению к общему количеству жизнеспособных бактерий наблюдалось в варианте с использованием 2 % галактоолигосахаридов ( P < 0.05). Результаты данного исследования доказывают возможность и эффективность использования галактоолигосахаридов в качестве пребиотика для обогащения кишечной бактериальной микрофлоры каспийской плотвы и мальков каспийского кутума.

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