RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal Industries2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1954410.22363/2312-797X-2020-15-1-62-85Research ArticleMolecular analysis of gibberellin receptor gene GID1 in Dasypyrum villosum and development of DNA marker for its identificationRazumovaOlga Vladimirovna<p>Candidate of Biological Sciences, Senior Researcher, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>razumovao@gmail.comBazhenovMikhail Sergeevich<p>Candidate of Biological Sciences, senior researcher, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>mikhabazhenov@gmail.comNikitinaEkaterina Aleksandrovna<p>Laboratory Assistant, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>shhket@gmail.comNazarovaLyubov Andreevna<p>Junior Researcher, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>lpukhova@yandex.ruRomanovDmitry Viktorovich<p>Candidate of Biological Sciences, senior researcher, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>akabos1987@gmail.comChernookAnastasiya Gennadievna<p>Junior Researcher, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>Irbis-sibrI@yandex.ruSokolovPavel Andreevich<p>Laboratory Assistant Researcher, Center for Molecular Biotechnology</p>pav2395147@yandex.ruKuznetsovaViktoria Maksimovna<p>Junior Researcher, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>vika-kuz367@yandex.ruSemenovOleg Grigorievich<p>Candidate of Biological Sciences, professor, Technosphere Safety Department, Agrarian-technological Institute</p>semenov_og@rudn.universityKarlovGennady Ilyich<p>Doctor of Biological Sciences, Professor, Academician of the Russian Academy of Sciences, Director</p>karlovg@gmail.comKharchenkoPetr Nikolaevich<p>Doctor of Biological Sciences, Professor, Academician of the Russian Academy of Sciences, Scientific Advisor</p>iab@iab.ruDivashukMikhail Georgievich<p>Candidate of Biological Sciences, leading researcher, Laboratory of Applied Genomics and Private Breeding of Agricultural Plants</p>divashuk@gmail.comAll-Russia Research Institute of Agricultural BiotechnologyMoscow Botanical Garden of Academy of SciencesRussian State Agrarian University - Moscow Timiryazev Agricultural AcademyРeoples’ Friendship University of Russia (RUDN University)15122020151628517032020Copyright © 2020, Razumova O.V., Bazhenov M.S., Nikitina E.A., Nazarova L.A., Romanov D.V., Chernook A.G., Sokolov P.A., Kuznetsova V.M., Semenov O.G., Karlov G.I., Kharchenko P.N., Divashuk M.G.2020<p>Dasypyrum villosum is an annual cereal used as a donor of agronomic traits for wheat. Productivity is one of the most important traits that breeding is aimed at. It is a very complex trait, the formation of which is influenced by many different factors, both internal (the genotype of the plant) and external. The genes responsible for the gibberellin sensitivity played a large role in multiplying yields of cereal crops. Another such gene is the Gid1, which encodes a receptor for gibberellins. This article compares the DNA sequences of the Gid1 gene obtained from six Dasypyrum villosum samples. Using a sequence of wheat and rye taken from the GenBank database (NCBI), we selected primers for regions of different genomes (A, B, and D subgenomes of wheat and the R genome of rye), and carried out a polymerase chain reaction on D. villosum accessions of diverse geographical origin. The resulting PCR product was sequenced by an NGS method. Based on the assembled sequences, DNA markers have been created that make it possible to differentiate these genes of the V genome and homologous genes of wheat origin. Using monosomic addition, substitution, and translocation wheat lines, the localization of the Gid1 gene of D. villosum was established on the long arm of the first V chromosome. A phenotypic assessment of common wheat lines carrying substituted, translocated, or added D. villosum chromosomes in their karyotype was performed. Tendency of disappearance of the first chromosome of D. villosum in the lines with added chromosomes was revealed.</p>Dasypyrum villosumremote hybridizationGid1gibberellin receptorDasypyrum villosumGid1дазипирум мохнатыйотдаленная гибридизациярецепторгиббереллиновая кислота[Ray DK, Mueller ND, West PC, Foley JA.Yield trends are insufficient to double global crop production by 2050. 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