RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal IndustriesВестник Российского университета дружбы народов. Серия: Агрономия и животноводство2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1956110.22363/2312-797X-2020-15-2-134-141Plant protectionЗащита растенийResearch ArticleSpecific Identification Method based on PCR for Drosophila melanogasterИдентификация Drosophila melanogaster методом полимеразной цепной реакцииNaserzadehYousefНасерзадеЮсеф

Researcher, Agro-Biotechnological Department, Agrarian and Technological Institute

научный сотрудник агробиотехнологического департамента, Аграрно-технологический институт

unaserzadeh@gmail.comPakinaElena N.ПакинаЕлена Николаевна

Associate Professor, Candidate of Biological Sciences, Agro-Biotechnological Department, Agrarian and Technological Institute

кандидат биологических наук, доцент, агробиотехнологический департамент, Аграрно-технологический институт

e-pakina@yandex.ruNafchiAbdorreza M.НафчиАбдорреза Мохаммади

Professor

профессор

amohammadi@usm.myGadzhikurbanovAnvar Sh.ГаджикурбановАнвар Шихрагимович

Agroengineering Department, Agrarian and Technological Institute

агроинженерный департамент, Аграрно-технологический институт

gadcgikurbanow@mail.ruPeoples’ Friendship University of RussiaРоссийский университет дружбы народовAll-Russian Plant Quarantine CentreВсероссийский центр карантина растенийUniversity Sains MalaysiaНаучный университет Малайзии15122020152VOL 15, NO2 (2020)ТОМ 15, №2 (2020)13414113062020Copyright ©; 2020, Naserzadeh Y., Pakina E.N., Nafchi A.M., Gadzhikurbanov A.S.Copyright ©; 2020, Насерзаде Ю., Пакина Е.Н., Нафчи А.М., Гаджикурбанов А.Ш.2020Naserzadeh Y., Pakina E.N., Nafchi A.M., Gadzhikurbanov A.S.Насерзаде Ю., Пакина Е.Н., Нафчи А.М., Гаджикурбанов А.Ш.http://creativecommons.org/licenses/by/4.0https://agrojournal.rudn.ru/agronomy/article/view/19561

D. melanogaster is one of the most harmful citrus fruit flies having a large number of host plants. The molecular diagnostic method has been created for identification the D. melanogaster from another non-quarantine species Drosophila spp. The proposed method for differentiation is to use the mitochondrial DNA cytochrome oxidase I gene region 709-bp. We amplified samples of DNA with primers Droso-S391 and Droso-A381 by D. melanogaster, D. suzukii, and D. Simulans collections in the laboratory samples from many countries and contrasted with sequences of other GenBank Drosophila taxa. The findings of a polymerase chain reaction (PCR) based on DNA sequence polymorphisms showed that these primers accurately identify the area of the gene as well as the unique primers of Drosophila melanogaster.

D. melanogaster - одна из наиболее вредоносных плодовых мух, повреждающих цитрусовые и многие другие сельскохозяйственные растения. Молекулярный метод диагностики был создан для идентификации D. melanogaster от другого некарантинного вида Drosophila spp. Предложенный метод дифференциации заключается в использовании фрагмента гена цитохромоксидазы субъединицы 1 митохондриальной ДНК размером 709 п. н. С помощью праймеров Droso-S391 и Droso-A381 были амплифицированы образцы ДНК D. melanogaster, D. suzukii и D. Simulans в лабораторных образцах из разных стран, далее их сравнили с последовательностями других таксонов Drosophila из базы GenBank. Результаты проведения полимеразной цепной реакции (ПЦР) на основе полиморфизма нуклеотидных последовательностей показали, что данные праймеры точно идентифицируют участок гена, также как и специфические праймеры Drosophila melanogaster.

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