RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal Industries2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1973510.22363/2312-797X-2022-17-1-31-47Research ArticleAgrobacterium-mediated transformation of potato Solanum tuberosum L. with constructs carrying the strong plant-derived promoter pro-SmAMP1 from Stellaria media L.KhaliluevMarat R.<p>Candidate of biological sciences, Assistant professor, head of Plant cell engineering laboratory, Russian Research Institute of Agricultural Biotechnology; Assistant professor, Biotechnology Department, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy</p>marat131084@rambler.ruhttps://orcid.org/0000-0001-7371-8900KharchenkoPyotr N.<p>Academician of the Russian Academy of Sciences, Scientific Director</p>kharchenko@iab.ac.ruhttps://orcid.org/0000-0001-5074-0531OvchinnikovaVera N.<p>Candidate of biological sciences, Senior researcher, Plant cell engineering Laboratory</p>vera.ovchinnikova.1957@mail.ruhttps://orcid.org/0000-0003-0839-2048Russian Research Institute of Agricultural BiotechnologyRussian State Agrarian University - Moscow Timiryazev Agricultural Academy02042022171314702042022Copyright © 2022, Khaliluev M.R., Kharchenko P.N., Ovchinnikova V.N.2022<p style="text-align: justify;">The effectiveness of plant genetic transformation is determined by the choice of genetic structures and their regulatory sequences that cause a high and stable expression level of heterologous genes. In this regard, the actual task of biotechnology is the use of highly effective plant promoters. The choice of promoter determines not only the level of the expression gene, but also the effectiveness of genetic transformation. The purpose of our study was to evaluate the influence of explant type and 5-deletion variants of the plant strong pro-SmAMP1 promoter, on the Agrobacterium -mediated transformation efficiency of potato ( Solanum tuberosum L.) cv. Udacha. To analyze the regenerative capacity of potato stem and leaf explants, AGL0 strain carrying constructs containing the 5-deletion variants of the promoter fragment of gene encoding antimicrobial peptide from Stellaria media L. ( pro-SmAMP1 ) was carried out. Four genetic constructs based on the plant expression vector pCAMBIA1381Z were used in this work, containing the selectable gene hptII and reporter gene uidA under different 5-deletion variants of the pro-SmAMP1 promoter (-442, -675, -732 and -1196 bp relative to the transcription initiation site); as well as two binary vectors based on the expression vector pCAMBIA1302 with 5-deletion pro-SmAMP1 promoter variants (-442 and -1196 bp), controlling the expression of gfp reporter gene. It was found that the effectiveness of Agrobacterium -mediated transformation depended on the type of genetic construction used, but not on the type of explant being cultivated. The insertion of the promoter region pro-SmAMP1 gene, hptII , as well as the absence of the bacterial Vir E gene was confirmed by PCR. Depending on the type of genetic construct, the transformation efficiency for the reporter gene varied from 2.0 to 7.2 %. The results are compared with previously conducted few studies, according to which the choice of promoter determines not only the expression level of marker genes, but also has a significant influence on the genetic transformation efficiency.</p>Solanum tuberosum Lregenerative capacityAgrobacterium-mediated transformation35SCaMVpromoter pro-SmAMP1Stellaria media LSolanum tuberosum L35SCaMVStellaria media Lрегенерационный потенциалагробактериальная трансформацияпромотор pro-SmAMP1[Permyakova NV, Shumnyi VK, Deineko EV. Agrobacterium-mediated transformation of plants: Transfer of vector DNA fragments in the plant genome. Russian Journal of Genetics. 2009; 45(3):266—275. doi: 10.1134/S 1022795409030028][Gustafson V, Mallubhotla S, MacDonnell D, Sanyal-Bagchi M, Chakravarty D, Wang-Pruski G, et al. Transformation and plant regeneration from leaf explants of Solanum tuberosum L. cv. ‘Shepody’. 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