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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">RUDN Journal of Agronomy and Animal Industries</journal-id><journal-title-group><journal-title xml:lang="en">RUDN Journal of Agronomy and Animal Industries</journal-title><trans-title-group xml:lang="ru"><trans-title>Вестник Российского университета дружбы народов. Серия: Агрономия и животноводство</trans-title></trans-title-group></journal-title-group><issn publication-format="print">2312-797X</issn><issn publication-format="electronic">2312-7988</issn><publisher><publisher-name xml:lang="en">Peoples’ Friendship University of Russia named after Patrice Lumumba</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">19765</article-id><article-id pub-id-type="doi">10.22363/2312-797X-2022-17-2-193-202</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Animal breeding</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Животноводство</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Features of the functional state of the organism of sheep under stress</article-title><trans-title-group xml:lang="ru"><trans-title>Особенности функционального состояния организма овец при стрессе</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7150-1131</contrib-id><name-alternatives><name xml:lang="en"><surname>Yuldashbaev</surname><given-names>Yusupzhan A.</given-names></name><name xml:lang="ru"><surname>Юлдашбаев</surname><given-names>Юсупжан Артыкович</given-names></name></name-alternatives><bio xml:lang="en"><p>RAS Academician (Full Member), Doctor of Agricultural Sciences, Professor, Director of Institute of Zootechnics and Biology, Professor, Department of Private Animal Science</p></bio><bio xml:lang="ru"><p>доктор сельскохозяйственных наук, профессор, академик Российской академии наук, декан факультета зоотехнии и биологии, профессор кафедры частной зоотехнии</p></bio><email>zoo@rgau-msha.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0036-3402</contrib-id><name-alternatives><name xml:lang="en"><surname>Vatnikov</surname><given-names>Yury A.</given-names></name><name xml:lang="ru"><surname>Ватников</surname><given-names>Юрий Анатольевич</given-names></name></name-alternatives><bio xml:lang="en"><p>Doctor of Veterinary Sciences, Professor, Director of Veterinary Medicine Department, Agrarian and Technological Institute</p></bio><bio xml:lang="ru"><p>доктор ветеринарных наук, профессор, директор департамента ветеринарной медицины аграрно-технологического института</p></bio><email>vatnikov@yandex.ru</email><xref ref-type="aff" rid="aff2"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0418-9918</contrib-id><name-alternatives><name xml:lang="en"><surname>Rudenko</surname><given-names>Pavel A.</given-names></name><name xml:lang="ru"><surname>Руденко</surname><given-names>Павел Анатольевич</given-names></name></name-alternatives><bio xml:lang="en"><p>Doctor of Veterinary Sciences, Leading researcher, Biological Testing Laboratory, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences ; Associate Professor, Department of Veterinary Medicine, Agrarian and Technological Institute, Peoples’ Friendship University of Russia (RUDN University)</p></bio><bio xml:lang="ru"><p>доктор ветеринарных наук, ведущий научный сотрудник лаборатории биологических испытаний, Филиал Института биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН (ФИБХ РАН); доцент департамента ветеринарной медицины аграрно-технологического института, Российский университет дружбы народов (РУДН)</p></bio><email>pavelrudenko76@yandex.ru</email><xref ref-type="aff" rid="aff2"/><xref ref-type="aff" rid="aff3"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6434-3497</contrib-id><name-alternatives><name xml:lang="en"><surname>Rudenko</surname><given-names>Andrey A.</given-names></name><name xml:lang="ru"><surname>Руденко</surname><given-names>Андрей Анатольевич</given-names></name></name-alternatives><bio xml:lang="en"><p>Doctor of Veterinary Sciences, Professor; Department of Veterinary Medicine, Moscow State University of Food Production; Associate Professor, Department of Veterinary Medicine, Agrarian and Technological Institute, Peoples’ Friendship University of Russia (RUDN University)</p></bio><bio xml:lang="ru"><p>доктор ветеринарных наук, профессор кафедры ветеринарной медицины, Московский государственный университет пищевых производств (МГУПП); доцент департамента ветеринарной медицины аграрно-технологического института, Российский университет дружбы народов (РУДН)</p></bio><email>vetrudek@yandex.ru</email><xref ref-type="aff" rid="aff2"/><xref ref-type="aff" rid="aff4"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">Russian State Agrarian University - Moscow Timiryazev Agricultural Academy</institution></aff><aff><institution xml:lang="ru">Российский государственный аграрный университет - МСХА имени К.А. Тимирязева</institution></aff></aff-alternatives><aff-alternatives id="aff2"><aff><institution xml:lang="en">RUDN University</institution></aff><aff><institution xml:lang="ru">Российский университет дружбы народов</institution></aff></aff-alternatives><aff-alternatives id="aff3"><aff><institution xml:lang="en">Branch of Shemyakin - Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences</institution></aff><aff><institution xml:lang="ru">Филиал института биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук</institution></aff></aff-alternatives><aff-alternatives id="aff4"><aff><institution xml:lang="en">Moscow State University of Food Production</institution></aff><aff><institution xml:lang="ru">Московский государственный университет пищевых производств</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2022-06-16" publication-format="electronic"><day>16</day><month>06</month><year>2022</year></pub-date><volume>17</volume><issue>2</issue><issue-title xml:lang="en">VOL 17, NO2 (2022)</issue-title><issue-title xml:lang="ru">ТОМ 17, №2 (2022)</issue-title><fpage>193</fpage><lpage>202</lpage><history><date date-type="received" iso-8601-date="2022-06-16"><day>16</day><month>06</month><year>2022</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2022, Yuldashbaev Y.A., Vatnikov Y.A., Rudenko P.A., Rudenko A.A.</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2022, Юлдашбаев Ю.А., Ватников Ю.А., Руденко П.А., Руденко А.А.</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="en">Yuldashbaev Y.A., Vatnikov Y.A., Rudenko P.A., Rudenko A.A.</copyright-holder><copyright-holder xml:lang="ru">Юлдашбаев Ю.А., Ватников Ю.А., Руденко П.А., Руденко А.А.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">http://creativecommons.org/licenses/by/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://agrojournal.rudn.ru/agronomy/article/view/19765">https://agrojournal.rudn.ru/agronomy/article/view/19765</self-uri><abstract xml:lang="en"><p style="text-align: justify;">The stresses that arise during routine animal husbandry procedures, such as milking, shearing, weighing, loading and hoof care, are an actual problem in animal husbandry. The components of economic damage under stress are a decrease in overall resistance and productivity, deterioration in product quality, an increase in labor costs and funds per unit of production, an increase in animal morbidity and mortality. The studies were carried out on the basis of ‘Belozernoe’ farm in the Salsky district, the Rostov region. A complex of clinical and laboratory studies was performed on 18 sheep (10 Romanov and 8 Tsigay breeds) aged 2 to 3 years, before and after a planned routine shearing. Stress in sheep caused by planned shearing was clinically manifested by general depression, in some cases, subfebrile fever, moderate tachypnea and tachycardia. It was shown that stress was accompanied by anemia, the development of erythropenia and leukopenia. Immunocompetent cells in the blood of experimental animals during stress development were analyzed. Severe lymphocytopenia was found to occur after a haircut. In addition, in the blood of experimental animals under stress, a tendency to a decrease in T-total cells was noted, which was accompanied by an increase in 0-cells, which was significant in sheep of the Tsigay breed (p&lt;0.05). When analyzing the main immunoregulatory T cells in sheep under stress, a significant increase in T-suppressors was revealed with a relatively stable level of T-helpers in the blood studied. Thus, a significant (p&lt;0.05) increase in the level of T-suppressors in sheep of Romanov and Tsigay breeds was recorded; it was by 1.15 and 1.19 times higher compared with the initial data. It should be noted that we did not find any significant differences between animals of Romanov and Tsigay breeds in stress development after shearing. The obtained data should be taken into account in the further search for methods of stress correction in sheep.</p></abstract><trans-abstract xml:lang="ru"><p style="text-align: justify;">Стрессы, возникающие у животных при проведении рутинных животноводческих процедур, таких как доение, стрижка, взвешивание, погрузка и уход за копытами, - актуальная проблема животноводства. Компонентами экономического ущерба от стрессов являются снижение общей резистентности и продуктивности, ухудшение качества продукции, возрастание затрат труда и средств на единицу продукции, рост заболеваемости и смертности животных. Исследования проведены на базе племенного хозяйства ООО «Белозерное» Сальского района Ростовской области. Комплекс клинико-лабораторных исследований выполнен на 18 овцах (10 романовской и 8 цигайской породы) в возрасте от 2 до 3 лет, до и после проведения плановой рутинной стрижки. Стресс у овец, обусловленный плановой стрижкой, клинически проявлялся общим угнетением, в некоторых случаях - субфебрильным повышением температуры тела, умеренным тахипноэ и тахикардией. Показано, что стресс сопровождается анемией, развитием эритропении и лейкопении. Анализом иммунокомпетентных клеток в крови опытных животных при развитии стресса установлено, что после стрижки возникает выраженная лимфоцитопения. В крови опытных животных при стрессе отмечали тенденцию к снижению Т-общих клеток, что сопровождалось увеличением 0-клеток, которое у овец цигайской породы носило достоверный характер (р &lt; 0,05). При анализе основных иммунорегуляторных Т-клеток в исследуемой крови овец при стрессе при относительно стабильном уровне Т-хелперов выявлено достоверное (р &lt; 0,05) увеличение Т-супрессоров: у романовской - в 1,15, у цигайской в 1,19 раза по сравнению с исходными данными. Существенных отличий в развитии стресса после проведения стрижки у животных романовской и цигайской пород не обнаружено. Полученные данные необходимо учитывать в дальнейших поисках методов коррекции стресса у овец.</p></trans-abstract><kwd-group xml:lang="en"><kwd>stress</kwd><kwd>shearing</kwd><kwd>hematological analytes</kwd><kwd>sheep</kwd><kwd>Romanov breed</kwd><kwd>Tsigay breed</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>стресс</kwd><kwd>стрижка</kwd><kwd>гематологические аналиты</kwd><kwd>овцы</kwd><kwd>романовская порода</kwd><kwd>цигайская порода</kwd></kwd-group><funding-group/></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Noguera JC, Kim SY, Velando A. Family-transmitted stress in a wild bird. Proc Natl Acad Sci. 2017; 114(26):6794-6799. doi: 10.1073/pnas.1706164114</mixed-citation></ref><ref id="B2"><label>2.</label><mixed-citation>Palikov VA, Palikova YA, Borozdina NA, Nesmeyanova EN, Rudenko PA, Kazakov VA, Kalabina EA, Bukatin MV, Zharmukhamedova TYu, Khokhlova ON, Dyachenko IA. A novel view of the problem of Osteoarthritis in experimental rat model. Research Results in Pharmacology. 2020;6(2):19-25. doi: 10.3897/ rrpharmacology.6.51772</mixed-citation></ref><ref id="B3"><label>3.</label><mixed-citation>Rudenko P, Sachivkina N, Vatnikov Y, Shabunin S, Engashev S, Kontsevaya S, Karamyan A, Bokov D, Kuznetsova O, Vasilieva E. Role of microorganisms isolated from cows with mastitis in Moscow region in biofilm formation. Veterinary World. 2021;14(1):40-48. doi: 10.14202/vetworld.2021.40-48</mixed-citation></ref><ref id="B4"><label>4.</label><mixed-citation>Vatnikov Y, Donnik I, Kulikov E, Karamyan A, Notina E, Bykova I, Bannoud G, Bondareva I, Shlindova E, Sotnikova E, Lenchenko E, Rudenko A, Rudenko V, Rudenko P. Effectiveness of Hypericum perforatum L. phytosorbent as a part of complex therapy for acute non-specific bronchopneumonia. International Journal of Pharmaceutical Research. 2020;12(S.1):1108-1116. doi: 10.31838/ijpr/2020.SP1.165</mixed-citation></ref><ref id="B5"><label>5.</label><mixed-citation>Arnaud F, Pappas G, Chen Y, Maudlin-Jeronimo E, McCarron R. Effect of acute restraint stress in a polytrauma rat model. Neurosci Lett. 2018;684:91-97. doi: 10.1016/j.neulet.2018.07.006</mixed-citation></ref><ref id="B6"><label>6.</label><mixed-citation>Frankham R. Stress and adaptation in conservation genetics. J Evol Biol. 2005;18(4):750-755. doi: 10.1111/j.1420-9101.2005.00885.x</mixed-citation></ref><ref id="B7"><label>7.</label><mixed-citation>Vatnikov Y, Yousefi M, Engashev S, Rudenko P, Lutsay V, Kulikov E, Karamyan A, Dremova T, Tadzhieva A, Strizhakov A, Kuznetsov V, Yagnikov S. Clinical and hematological parameters for selecting the optimal dose of the phytopreparation «Deprim», containing an extract of the herb Hypericum perforatum L., in husbandry. International Journal of Pharmaceutical Research. 2020;12(S.1):2731-2742. doi: 10.31838/ijpr/2020.SP1.401</mixed-citation></ref><ref id="B8"><label>8.</label><mixed-citation>Chen M, Xie S. Therapeutic targeting of cellular stress responses in cancer. Thorac Cancer. 2018; 9(12):1575-1582. doi: 10.1111/1759-7714.12890</mixed-citation></ref><ref id="B9"><label>9.</label><mixed-citation>Banerjee B, Khrystoforova I, Polis B, Zvi IB, Karasik D. Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism. Cell Mol Life Sci. 2021;79(1):41. doi: 10.1007/s00018-021-04043-x</mixed-citation></ref><ref id="B10"><label>10.</label><mixed-citation>Yardimci M, Sahin EH, Cetingul IS, Bayram I, Aslan R, Sengor E. Stress responses to comparative handling procedures in sheep. Animal. 2013;7(1):143-150. doi: 10.1017/S 1751731112001449</mixed-citation></ref><ref id="B11"><label>11.</label><mixed-citation>Broom DM. Transport stress in cattle and sheep with details of physiological, ethological and other indicators. Dtsch Tierarztl Wochenschr. 2003; 110(3):83-89.</mixed-citation></ref><ref id="B12"><label>12.</label><mixed-citation>Escribano D, Horvatić A, Contreras-Aguilar MD, Guillemin N, Cerón JJ, Lopez-Arjona M, Hevia ML, Eckersall PD, Manteca X, Mrljak V. Identification of possible new salivary biomarkers of stress in sheep using a high-resolution quantitative proteomic technique. Res Vet Sci. 2019;124:338-345. doi: 10.1016/j.rvsc.2019.04.012</mixed-citation></ref><ref id="B13"><label>13.</label><mixed-citation>Gong S, Miao YL, Jiao GZ, Sun MJ, Li H, Lin J, Luo MJ, Tan JH. Dynamics and correlation of serum cortisol and corticosterone under different physiological or stressful conditions in mice. PLoS One. 2015;10(2): e0117503. doi: 10.1371/journal.pone.0117503</mixed-citation></ref><ref id="B14"><label>14.</label><mixed-citation>Hemsworth PH, Rice M, Borg S, Edwards LE, Ponnampalam EN, Coleman GJ. Relationships between handling, behaviour and stress in lambs at abattoirs. Animal. 2019;13(6):1287-1296. doi: 10.1017/S 1751731118002744</mixed-citation></ref><ref id="B15"><label>15.</label><mixed-citation>Karthik D, Suresh J, Reddy YR, Sharma GRK, Ramana JV, Gangaraju G, Reddy YPK, Yasaswini D,Adegbeye M J, Reddy PRK. Farming systems in sheep rearing: Impact on growth and reproductive performance, nutrient digestibility, disease incidence and heat stress indices. PLoS One. 2021;16(1): e0244922. doi: 10.1371/ journal.pone.0244922</mixed-citation></ref><ref id="B16"><label>16.</label><mixed-citation>Tozlu Çelik H, Aslan FA, Us Altay D, Kahveci ME, Konanç K, Noyan T, Ayhan S. Effects of transport and altitude on hormones and oxidative stress parameters in sheep. PLoS One. 2021;16(2): e0244911. doi: 10.1371/journal.pone.0244911</mixed-citation></ref><ref id="B17"><label>17.</label><mixed-citation>Wankar AK, Rindhe SN, Doijad NS. Heat stress in dairy animals and current milk production trends, economics, and future perspectives: the global scenario. Trop Anim Health Prod. 2021;53(1):70. doi: 10.1007/s11250-020-02541-x</mixed-citation></ref><ref id="B18"><label>18.</label><mixed-citation>Henrique FL, Bezerra HVA, Polato HZ, Fernandes AC, Zanella AJ, Alves MBR, Celeghini ECC,Batissaco L, Strefezzi RFS, Pulido-Rodríguez LF, Hooper HB, Titto CG. Maternal stress in sheep during late pregnancy influences sperm quality in early puberty of the offspring. Theriogenology. 2020;145:158-166. doi: 10.1016/j.theriogenology.2019.10.008</mixed-citation></ref><ref id="B19"><label>19.</label><mixed-citation>Carnevali L, Montano N, Tobaldini E, Thayer JF, Sgoifo A. The contagion of social defeat stress: Insights from rodent studies. Neurosci Biobehav Rev. 2020; 111:12-18. doi: 10.1016/j.neubiorev.2020.01.011</mixed-citation></ref><ref id="B20"><label>20.</label><mixed-citation>Grzelak AK, Davis DJ, Caraker SM, Crim MJ, Spitsbergen JM, Wiedmeyer CE. Stress leukogram induced by acute and chronic stress in zebrafish. Comp Med. 2017;67(3):263-269.</mixed-citation></ref></ref-list></back></article>
