RUDN Journal of Agronomy and Animal IndustriesRUDN Journal of Agronomy and Animal Industries2312-797X2312-7988Peoples’ Friendship University of Russia named after Patrice Lumumba1976610.22363/2312-797X-2022-17-2-203-209Research ArticleTrace element status of highly productive cows in different physiological conditions under increased technogenic loadDrozhzhinOleg S.<p>Candidate of Biological Sciences, Leading Researcher, Laboratory of toxicology, safety risk assessment of raw materials and products of biological Origin</p>icrsa@mail.ruhttps://orcid.org/0000-0002-0354-8149ShipilovValeriy V.<p>Engineer for adjustment and testing, Laboratory of toxicology, risk assessment of the safety of raw materials and products of biological origin</p>vnivipat@mail.ruhttps://orcid.org/0000-0003-0730-3680Russian Research Veterinary Institute of Pathology, Pharmacology and Therapy1606202217220320916062022Copyright © 2022, Drozhzhin O.S., Shipilov V.V.2022<p style="text-align: justify;">Microelements play a vital role in the maintenance and intensification of metabolic and regulatory processes in the body of animals. For a more complete study of provision of body of highly productive animals with microelements and diagnosis of metabolic disorders, especially under anthropogenic load, it is necessary to conduct biochemical blood tests. The article presents the results of studies on the content of trace elements (iron, copper, zinc, manganese) in the whole blood of cows from two farms in the Voronezh region, one of which (farm 2) is located in an area with increased technogenic load. In whole blood, the content of iron, copper, zinc, and manganese was determined by atomic absorption using a Shimadzu AA-6300 spectrophotometer. Samples were prepared by wet ashing at elevated pressure in a MARS-5 microwave system. Blood samples were taken 2 weeks before calving, one week and one month after calving. A reduced level of copper and zinc was found in animals from both farms throughout the entire study period. The dependence of the content of microelements on the physiological state of animals (before and after calving) was not revealed. It was shown that the content of iron in the blood of highly productive cows from farm 2 was lower throughout the study, and manganese - a week and a month after calving. The amount of manganese in blood of farm 2 animals was less than the lower limit of the physiological norm, while in farm 1 animals its concentration was within the reference normal range.</p>cowswhole bloodtrace elementsironcopperzincmanganesetechnogenic loadphysiological stateкоровыцельная кровьмикроэлементыжелезомедьцинкмарганецтехногенная нагрузкафизиологическое состояние[Miroshnikov SA, Skalny AV, Zavyalov OA, Frolov AN, Grabeklis AR. The reference values of hair content of trace elements in dairy cows of Holstein breed. Biol Trace Elem Res. 2020;194(1):145-151. doi: 10.1007/s12011-019-01768-6][Vatnikov Y, Donnik I, Kulikov E, Karamyan A, Notina E, Bykova I, Bannoud G, Bondareva I, Shlindova E, Sotnikova E, Lenchenko E, Rudenko A, Rudenko V, Rudenko P. Effectiveness of Hypericum perforatum L. phytosorbent as a part of complex therapy for acute non-specific bronchopneumonia. International Journal of Pharmaceutical Research. 2020; 12(S.1):1108-1116. doi: 10.31838/ijpr/2020.SP1.165][Sheppard SC, Sanipelli B. Trace elements in feed, manure, and manured soils. J Environ Qual. 2012; 41(6):1846-1856. doi: 10.2134/jeq2012.0133][Rudenko P, Sachivkina N, Vatnikov Y, Shabunin S, Engashev S, Kontsevaya S, Karamyan A, Bokov D, Kuznetsova O, Vasilieva E. Role of microorganisms isolated from cows with mastitis in Moscow region in biofilm formation. Veterinary World. 2021;14(1):40-48. doi: 10.14202/vetworld.2021.40-48][Diyabalanage S, Kalpage MD, Mohotti DG, Dissanayake CKK, Fernando R, Frew RD, Chandrajith R. Comprehensive assessment of essential and potentially toxic trace elements in bovine milk and their feeds in different agro-climatic zones of Sri Lanka. Biol Trace Elem Res. 2021;199(4):1377-1388. doi: 10.1007/s12011-020-02242-4][Omur A, Kirbas A, Aksu E, Kandemir F, Dorman E, Kaynar O, Ocar O. Effects of antioxidant vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) on some metabolic and reproductive profiles in dairy cows during transition period. Pol J Vet Sci. 2016;19(4):697-706. doi: 10.1515/pjvs-2016-0088][Pereira V, Carbajales P, López-Alonso M, Miranda M. Trace element concentrations in beef cattle related to the breed aptitude. Biol Trace Elem Res. 2018;186(1):135-142. doi: 10.1007/s12011-018-1276-3][Vatnikov Y, Shabunin S, Kulikov E, Karamyan A, Murylev V, Elizarov P, Kuznetsova O, Vasilieva E, Petukhov N, Shopinskaya M, Rudenko A, Rudenko P. The efficiency of therapy the piglets gastroenteritis with combination of enrofloxacin and phytosorbent Hypericum perforatum L. International Journal of Pharmaceutical Research. 2020;12(S.2):3064-3073. doi: 10.31838/ijpr/2020.SP2.373][Miroshnikov S, Notova S, Kazakova T, Marshinskaia O. The total accumulation of heavy metals in body in connection with the dairy productivity of cows. Environ Sci Pollut Res Int. 2021;28(36):49852-49863. doi: 10.1007/s11356-021-14198-6][Yazlık MO, Çolakoğlu HE, Pekcan M, Kaya U, Küplülü Ş, Kaçar C, Polat M, Vural MR. Effects of injectable trace element and vitamin supplementation during the gestational, peri-parturient, or early lactational periods on neutrophil functions and pregnancy rate in dairy cows. Anim Reprod Sci. 2021;225:106686. doi: 10.1016/j.anireprosci.2021.106686][Gruber A, Müller R, Wagner A, Colucci S, Spasić MV, Leopold K. Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples. Anal Bioanal Chem. 2020;412(24):6419-6429. doi: 10.1007/s00216-020-02614-8][Vatnikov Y, Yousefi M, Engashev S, Rudenko P, Lutsay V, Kulikov E, Karamyan A, Dremova T, Tadzhieva A, Strizhakov A, Kuznetsov V, Yagnikov S. Clinical and hematological parameters for selecting the optimal dose of the phytopreparation «Deprim», containing an extract of the herb Hypericum perforatum L., in husbandry. International Journal of Pharmaceutical Research. 2020;12(S.1):2731-2742. doi: 10.31838/ijpr/2020.SP1.401][Gonçalves DA, Soncin AC, Donati GL, dos Santos MC. Determination of trace elements in cow placenta by tungsten coil atomic emission spectrometry. Biol Trace Elem Res. 2017;178(2):228-234. doi: 10.1007/ s12011-016-0926-6][Saribal D. ICP-MS analysis of trace element concentrations in cow’s milk samples from supermarkets in Istanbul, Turkey. Biol Trace Elem Res. 2020;193(1):166-173. doi: 10.1007/s12011-019-01708-4][Palikov VA, Palikova YA, Borozdina NA, Nesmeyanova EN, Rudenko PA, Kazakov VA, Kalabina EA, Bukatin MV, Zharmukhamedova TYu, Khokhlova ON, Dyachenko IA. A novel view of the problem of Osteoarthritis in experimental rat model. Research Results in Pharmacology. 2020;6(2):19-25. doi: 10.3897/ rrpharmacology.6.51772][Vatnikov Y, Donnik I, Kulikov E, Karamyan A, Sachivkina N, Rudenko P, Tumanyan A, Khairova N, Romanova E, Gurina R, Sotnikova E, Bondareva I. Research on the antibacterial and antimycotic effect of the phytopreparation Farnesol on biofilm-forming microorganisms in veterinary medicine. International Journal of Pharmaceutical Research. 2020;12(S.2):1481-1492. doi: 10.31838/ijpr/2020.SP2.164]