Junior Researcher, Mycology and Helminthology Department
младший научный сотрудник научно-методического отдела микологии и гельминтологии
Junior Researcher, Scientific and Methodological Department of Mycology and Helminthology
младший научный сотрудник научно-методического отдела микологии и гельминтологии
Junior Researcher, Laboratory of Bacteriology and Analysis of Genetically Modified Organisms, Testing Laboratory Center
младший научный сотрудник лаборатории бактериологии и анализа генетически модифицированных организмов испытательного лабораторного центра
Junior Researcher, Molecular Genetics Shared Use Center
младший научный сотрудник центра коллективного пользования «Молекулярная генетика»
Strawberry anthracnose, caused by fungi of the Colletotrichum acutatum species complex, is one of the most aggressive diseases affecting this crop worldwide. With the intensification of production and growing demand for fresh berries, the development of control methods that ensure high quality and environmentally safe production while maintaining cost-effectiveness is becoming increasingly urgent. Bioagents represent a promising solution for strawberries, given their short vegetative period until fruit bearing and the fact that berries are primarily consumed fresh. The aim of this study was to identify bacterial strains as potential biocontrol agents against anthracnose pathogens. Fungi of the C. acutatum complex were isolated from planting material using a moist chamber technique and plating on nutrient medium. Bacterial potential antagonists were obtained from the collection of All-Russian Plant Quarantine Center. Pathogen identification was carried out using cultural and morphological methods, with confirmation by Sanger sequencing. Antagonistic activity was assessed using the dual-culture method, and the biochemical profile of the bacteria was determined using the API 20 E test system (bioMérieux, France). The most active isolates were: 0739-Bacillus velezensis, 0084-Bacillus halotolerans/mojavensis, 0075-Bacillus stercoris/subtilis, 0585-Bacillus subtilis, 0089-Bacillus subtilis/Azohydromonas sediminis, 0076-Azohydromonas sediminis/Bacillus subtilis, and 0552-Bacillus pumilus. Analysis of literature data indicates the absence of phytotoxicity in the selected strains. Biochemical testing revealed the absence of tryptophan-dependent indole‑3‑acetic acid synthesis pathway, which is characteristic of pathogens, and demonstrated high adaptability through the ability to utilize a wide range of substrates. The obtained data suggest that the isolates possess plant growth-promoting and stress tolerance-enhancing properties. High antagonistic activity, potential absence of phytotoxicity, adaptability, and growth-stimulating potential make these strains a promising basis for developing a biocontrol agent against strawberry anthracnose.
Антракноз земляники, вызываемый грибами комплекса