RUDN Journal of Agronomy and Animal Industries
Editor-in-Chief: Yuri A. Vatnikov, Doctor of Veterinary Sciences, Professor ISSN: 2312-797Х (Print) ISSN: 2312-7988 (Online) Indexation: Russian Index of Science Citation, RSCI, CABI, AGRIS, WJCI, Google Scholar, Ulrich's Periodicals Directory, WorldCat, Cyberleninka, DOAJ, Dimensions, ResearchBib, Research4Life, Lens, JournalTOCs Founded in 2006. Publication frequency: quarterly. Publication language: Russian, English Peer-Review: double blind. Open Access: Open Access. APC: no article processing charge. PUBLISHER: Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)
Peoples’ Friendship University of Russia named after Patrice Lumumba
The right of authorship belongs to the authors of articles. The authors transfer the rights to use the article (including the use and distribution of an article in the Open Access) to the publisher of the journal on a non-exclusive license (Publishing Agreement (Public Offer)). At the same time, authors remain full rightsholders. Publishing Agreement (Public Offer) to Publish an Article in an Academic Periodical "RUDN Journal of Agronomy and Animal Indastries" The Federal State Autonomous Educational Institution of Higher Education "Peoples’ Friendship University of Russia named after Patrice Lumamba” (RUDN University), represented by the Vice-Rector for Scientific Work Andrey Aleksandrovich Kostin, acting under a Power of Attorney No 0044-09/20-198 dated 29.07.2020, hereinafter referred to as the Publisher, on the one hand, hereby offers to the public at large, hereinafter referred to as the Author, on the other hand, hereinafter collectively referred to as the Parties, to enter into this agreement, hereinafter referred to as the Agreement, regarding publication of scholarly materials, hereinafter referred to as the Article, in the Journal named 'RUDN Journal of Agronomy and Animal Industries', hereinafter referred to as the Journal, under the following terms. 1. General Terms and Conditions 1.1. Pursuant to Clause 2 of the Article 437 of the Russian Federation Civil Code, this Agreement shall be recognized as the public offer, hereinafter referred to as the Offer. Subject to Article 438 of the Russian Federation Civil Code, this Offer shall be deemed to have been completely and irrevocably accepted after the Author had submitted his/her materials by uploading those onto the network electronic system of the article acquisition for review available at the respective section of the Journal's site at URL:http://journals.rudn.ru/index.php/agronomy/author/submit/1 on the Information and Communication Network known as the Internet, hereinafter referred to as the Internet. 1.2. 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The Offer Acceptance means a complete and irrevocable acceptance of the Offer under the terms specified in Clause 3 hereof (the Author accepts the Offer by submitting an application to the Publisher, i.e. by uploading the Article along with support materials onto the network electronic system of the article acquisition for review available at the respective section of the Journal's site at URL: http://journals.rudn.ru/index.php/agronomy/author/submit/1 on the Internet). The Journal means an academic periodical named '<span lang="EN-US" style="background-attachment: scroll; background-clip: border-box; background-color: transparent; background-image: none; background-origin: padding-box; background-position-x: 0%; background-position-y: 0%; background-repeat: repeat; background-size: auto; color: #101010; font-family: Arial; font-size: 14px; font-style: normal; font-variant: normal; font-weight: 400; letter-spacing: normal; orphans: 2; outline-color: invert; outline-style: none; outline-width: 0px; text-align: left; text-decoration: none; text-indent: 0px; text-transform: none; vertical-align: baseline; -webkit-text-stroke-width: 0px; white-space: normal; word-spacing: 0px; padding: 0px; margin: 0px;">RUDN Journal of Agronomy and Animal Industries'. 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The Article means a result of fundamental and applied scholarly efforts in the form of a scholarly material, scientific review material, scientific message, bibliographical review on specific topics of the scholarly study, and background information on Russian and foreign scientists submitted by the Author for publication in the Journal. The Parties mean the Author and the Publisher. The Article Requirements mean requirements to the materials published in the Journal's section named "To Authors" of the Journal's site at URL: http://journals.rudn.ru/index.php/agronomy/about/submissions#authorGuidelines on the Internet. The Service means publication of the Article in the Journal as per the Author's application. 3. Subject Matter of the Agreement (Offer) 3.1. Subject to this Agreement, the Author shall provide the Publisher at no expense for the copyright duration as stated by the Russian Federation legislation with a non-exclusive license for use of the Author-created Article for the purpose of its publication in the Journal. 3.2. The rights to use this Article as assigned hereunder shall include but not limited to: reproduction of the Article or any part thereof, as well as its metadata in Russian and English in any material form whatsoever, including soft and hard copy forms, as an individual work product in periodicals and/or databases (whether local or web-based) owned by the Publisher and/or any other persons at the discretion of the Publisher; dissemination of the Article or any part thereof, as well as its metadata in Russian and English on any media as part of the Journal and/or databases owned by the Publisher or any other persons, at the discretion of the Publisher, or as an individual work product, worldwide via open access or by subscription without payment of any fee to the Author; making public the Article or any part thereof as well as its metadata in Russian and English so that any person could access the Article from any location and at any time of his/her choice (including via the Internet); issuing permissions to use the Article or any part thereof as well as its metadata in Russian and English to any third parties while notifying the Author by publishing the respective information on the Journal's site without payment of any fee to the Author; processing, including translation of the Article (including translation into foreign languages), and use of the processed (translated) Article in any manner specified above; any other rights, not expressly assigned to the Publisher hereunder, including patent rights to any processes, techniques or methods, and other means, described by the Author in the Article, as well as trademark rights, shall be retained by the Author. 3.3. No territorial restrictions will be applied to the use of rights to the Article. 3.4. This Agreement shall become effective from the time the Article is submitted to the Journal, i.e. uploaded along with support materials onto the network electronic system of the article acquisition for review available at the relevant section of the Journal's site at URL: http://journals.rudn.ru/index.php/agronomy/author/submit/1 on the Internet, hereinafter referred to as the Article Upload. 3.5. The rights shall be assigned by the Author to the Publisher at no expense, and the Article publication in the Journal shall not result in any financial contribution to the Author. 3.6. If the Publisher decides to refuse to publish the Article in the Journal, this Agreement shall cease to be in force. A decision to refuse publication shall be sent to the Author via the e-mail address specified in the Application. 3.7. The Publisher undertakes, throughout the currency of this Agreement, to provide to the Author the services associated with publication of the Article on the Journal's site at URL: http://journals.rudn.ru/agronomy/index on the Internet. 4. General Terms of the Services Provision 4.1. The Publisher shall provide the services to the Author only if: the Author submits all materials compliant with the Offer requirements by way of the Article Upload; the Author Accepts the Offer. 4.2. The services shall be provided to the Author free of charge. 4.3. If any materials submitted by the Author are found in breach of rules and requirements of this Offer, the Publisher shall have a right to refuse to publish those. 4.4. The Publisher shall not be responsible for any third-party unauthorized use of the data provided by the Author during the currency of this Agreement. 5. Rights and Responsibilities of the Parties 5.1. The Author warrants that: he/she is the valid holder of exclusive rights to the Article; rights granted to the Publisher hereunder were not earlier assigned and will not be assigned to any third parties prior to publication of the Article by the Publisher in the Journal; the Article contains all the references to cited authors and/or sources (materials) that are required by the effective copyright law; the Author has obtained all required licenses to results, facts and other borrowed materials used in the Article where the Author is not a copyright holder; the Article does not contain any materials that may not be published in public sources in accordance with the effective Russian Federation statutory instruments, and the Article publication and dissemination will not involve any disclosure of classified (confidential) information, including state secrets; the Author has familiarized himself with the editorial policy and ethical principles published on the Journal website (http://agrojournal.rudn.ru/agronomy/about/editorialPolicies) and the consequences of violating these principles, has advised other Co-authors of the terms of this Agreement and has received consents from all the Co-authors to conclude this Agreement under the terms hereunder. 5.2. 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The Publisher is entitled to: carry out technical and literary editing of the Article such that its basic content would remain unchanged; review the Article and suggest appropriate changes to the Author, and elect not to publish the Article if the Author fails to introduce such changes; require from the Author and/or other persons that the Journal, Publisher, Author and any other copyright holder, as well as the title of the Article, Journal issue identification and a year of publication as specified in the Journal be properly referenced whenever the Journal and/or the Article, including any individual part or fragment thereof, are used by any of the above-mentioned individuals thereafter; publish preliminary and/or advertising information on the forthcoming publication of the Article in mass media and any other information sources; establish rules (conditions) for acceptance and publication of materials in the Journal. The Journal's Editorial Board, headed by the Editor-in-Chief, shall enjoy an exclusive right to accept and/or reject any materials submitted to the Journal's Editorial Office for publication purposes. No manuscript (tangible medium) submitted by the Author to the Journal's Editorial Office will be subject to return. The Journal's Editorial Staff will not enter into any correspondence regarding the rejection of the Article by the Journal's Editorial Board; suspend provision of services to the Author hereunder temporarily for technical, technological and other reasons preventing the services from being provided for the duration of remedial activities; suspend services hereunder in accordance with civil procedures if: the Article does not correspond to the topics covered by the Journal or by any part thereof, or the submitted material is found insufficient for the individual publication, or the Article design is found at variance with the requirements imposed; the Author is in breach of any other obligations assumed under the Offer arrangement; introduce changes to the Offer as per the procedure established by the Offer. 5.6. Unless otherwise provided or stated herein, the Parties shall refer to the effective legislation of the Russian Federation. 6. Offer Acceptance and Conclusion of the Agreement. The Term of the Agreement 6.1. This Agreement shall become effective when concluded, i.e. when the Author Accepts the Offer by sending an application to the Publisher, that is, by uploading the Article (Article Upload), and remain in effect for five years. 6.2. The Offer Acceptance constitutes an Agreement made in writing (Articles 438 and 1286.1 of the Russian Federation Civil Code) under the Offer terms. 6.3. Unless any of the Parties sends to the other Party a written notification of the Agreement termination no later than two months prior to the end of the specified five-year period, the duration of the Publisher's copyright to the Work shall be automatically extended by the similar period. The number of extensions is not limited. 6.4. The term of the Agreement may not exceed the duration of exclusive rights to the Article pursuant to the Russian Federation legislation. 6.5. Whenever the Author assigns (disposes of) its exclusive rights to the Work to any third party, this Agreement shall remain in effect. 7. Agreement Modification and Termination Procedure 7.1. The Publisher shall have a right to unilaterally modify the terms of this Agreement by giving a prior notice to the Author, at least ten (10) calendar days before such modifications become effective, to the Author's e-mail address specified in the Author's application or via the Journal's site. These modifications shall become effective from the date specified in the respective notice. 7.2. If the Author disagrees with any modification in the terms of this Agreement, the Author shall have a right to submit a written notice of his/her withdrawal from this Agreement by uploading it onto the network electronic system of the article acquisition for review, available at the relevant section of the Journal's site at URL: http://journals.rudn.ru/index.php/agronomy/author/submit/1 on the Internet. 7.3. 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Any notifications, messages, requests etc., excluding documents which need to be forwarded in original form pursuant to the Russian Federation legislation, shall be deemed to have been received by the Author, if delivered (sent) by the Publisher via the Journal's site, including by publishing those, by fax or e-mail specified in the Application, or using other communication facilities. The Parties acknowledge validity of notifications, messages, requests etc. delivered (sent) using the above-listed methods. 10.2. Where the Publisher becomes subject to any claims related to non-compliance with an exclusive copyright or other intellectual property right of any third party during the writing of the Article or in connection with conclusion of this Agreement by the Author, the Author undertakes to: immediately upon receipt of a Publisher's notification, take measures to settle disputes with such third party, and, where necessary, join the litigation in favor of the Publisher and use its best endeavors to exclude the Publisher from the defendants; reimburse the legal cost, expenses and loss incurred by the Publisher as a result of application of a pre-award relief and measures for execution of a judgment, and damages paid by the Publisher to any third party for the infringement of exclusive copyright and/or other intellectual property rights, as well as other expenditures incurred by the Publisher as a result of infringement by the Author of any warranties provided hereunder. 10.3. Subject to Article 6 of the Federal Law On Personal Data No. 152-FZ dated July 27, 2006, for the period from the conclusion of this Agreement and until the discharge of Parties obligations hereunder, the Author expresses his/her consent to processing of the following personal data by the Publisher: last name, first name, patronymic; taxpayers identification number (TIN); place and date of birth; citizenship details; ID document details; registration and actual location address; e-mail address; mailing address with postal code; contact phone numbers; fax numbers; and details of the employers. 10.4. The Publisher is entitled to process the above personal data for the purpose of this Agreement performance, including for provision of information and reference services to the Author. The processing of personal data shall be understood to mean any handing (operations) with personal data, including collection, systematization, accumulation, storage, refinement (update, alteration), use, distribution (including transfer to third parties), depersonalization, blocking and deletion of personal data in accordance with the effective Russian Federation legislation. 10.5. Where permitted by the Russian Federation legislation, the Author is entitled to withdraw his/her consent to processing of personal data as listed in Clause 10.3 by forwarding a respective notice to the Publisher. Upon receipt of such notice, the Publisher has a right to suspend services. 10.6. The Author will, on a goodwill basis, submit his/her details (and details of each Co-Author subject to their prior consent) to the Journal's Editorial Office consisting of his/her: last name, first name, patronymic, academic rank, academic degree, job title, employer details (name and mailing address), work phone number and e-mail address for the purpose of its general publication in the Journal along with the Article. 11. Registered Address and Particulars of the Publisher Federal State Autonomous Educational Institution of Higher Education "Peoples’ Friendship University of Russia named after Patrice Lumumba” (RUDN University) Address: 6 Mikluho-Maklaya str, Moscow, 117198, Russian Federation TIN 7728073720, CRR 772801001, PSRN 1027739189323. Vice Rector for Scientific Work A.A. Kostin
Copyright (c) 2025 Vatnikov Y.A. https://creativecommons.org/licenses/by-nc/4.0
Hormonal drugs for veterinary usein the pharmaceutical market: assortment analysis
Medicines are successfully used to correct physiological and biochemical processes, primarily to intensify the development of the animal organism in agriculture. Registered medicinal products for veterinary use comprises 2384 units. Modern pharmacology has a wide range of drugs that contribute to faster development to increase the muscle mass of the animal and significantly reduce costs for obtaining marketable products. Hence the attention paid to hormonal drugs by zootechnical and veterinary services. The results of a marketing study of the veterinary use drugs range that are offi registered in the specialized market of the Russian Federation are presented. The study was conducted to ensure the target consumer market. The results obtained will increase the awareness of specialized specialists, expand their professional horizons and, as a result, improve the coordination of material, labor and financial assets of the veterinary organization and its activities in general.
Copyright (c) 2025 Belousov E.A., Novikova E.O., Karasev M.M., Belousova O.V., Notina E.A., Novikov O.O. https://creativecommons.org/licenses/by-nc/4.0
Evaluation of the effectiveness of a general tonic feed additive for minks under industrial production conditions
Feeding balanced in amino acids, micro and macro elements, vitamins and other biologically active substances is a necessary condition for the productivity and development of fur animals, as well as for obtaining high-quality fur raw materials. Analysis of the amino acid profile of the main productive diet of the mink at JSC Fur Farm “Saltykovsky” revealed a deficiency of the essential amino acids: histidine, tryptophan and threonine, when maintaining a total protein within the normal range. To correct protein metabolism, a hydrolysate from sable and mink carcasses after technological euthanasia and skinning was included in the diet of minks. The hydrolysate is sterile, does not have pyrogenicity, acute and chronic toxicity, does not cause allergic reactions, contains 18 amino acids, including 10 essential, 6 macronutrients (Ca, Na, S, Mg, K, P) and 18 trace elements. A scientific and economic experiment was carried out by a commission on the basis of JSC Fur Farm “Saltykovsky” on standard wild mink 3 months of age, of which 1 control and 2 experimental groups of 120 animals each (60 males and 60 females) were formed. The results of the experiment showed that the feed additive in doses of 2 and 4 ml of a 15% solution per capita mixed with feed of the productive diet once a day for 4 months has a tonic effect on the mink organism.
Copyright (c) 2025 Denisenko V.N., Abramov P.N., Balakirev N.A., Albulov A.I., Frolova M.A. https://creativecommons.org/licenses/by-nc/4.0
Market analysis of veterinary medicines for dogs
Medicinal products intended for dogs were studied. According to the statistics of the AllRussian Public Opinion Research Center1, every third family in Russia has a dog. In this regard, maintaining the health of dogs is of great importance both from a psychological and social perspective. The current state of the Russian veterinary market is characterized by a reduction in the number of foreign suppliers. For example, in 2017, there were 867 veterinary drugs of foreign origin in Russia, of which approximately 180 items remained in market, but this number tend to decrease. The shares of foreign and Russian drugs presented in the Russian market are approximately equal, which indicates a great dependence on foreign suppliers. At the same time, many veterinarians prefer to use foreign drugs as the most effective and safe. However, Russian drugs recently appear in the Russian market are corresponding in quality to foreign analogues. Nowadays, they should form the basis for import substitution. The priority for import substitution should be drugs for the treatment of the most common and deadly diseases of dogs, the list of which is established by the national standard of the Russian Federation (GOST R 70040-2022) 2. According to the list, 35 of the most common diseases in practice were identifi for 10 organs and systems of dogs. The method of grading the medicinal products quality previously developed by the authors was used to select medicinal products by a treatment way. As a result, 353 drugs were selected, including 178 foreign and 175 Russian products. It was established that the supply of foreign drugs by the origin of medicines comprises 34 foreign countries, including 8 friendly and 26 unfriendly. At the same time, 148 drugs are supplied from unfriendly countries, and only 30 from friendly ones. The current trend of reducing supplies from unfriendly countries poses a threat of a shortage of medicines to Russia. 1 Russian Public Opinion Research Center (VCIOM). URL: https://wciom.ru/analytical-reviews/ analiticheskii-obzor/publichnaja-zhizn-domashnikh-zhivotnykh (date of access: 29.06.2025). 2 GOST R 70040-2022. National Standard of the Russian Federation "Classification of animal diseases of the canine and feline families". Moscow, 2022.
Copyright (c) 2025 Kolycheva A.G., Rudenko A.A., Shopinskaya M.I. https://creativecommons.org/licenses/by-nc/4.0
Prooxidant-antioxidant control of the effectiveness of aerosol therapy for acute catarrhal bronchopneumonia in calves
The results of prooxidant-antioxidant control of the efficiency of various schemes of aerosol complex therapy for acute catarrhal bronchopneumonia in calves using the generally accepted scheme (aerosol treatment indoors with iodotriethyleneglycol solution with intramuscular administration of the drug ”Penstrep‑400“), as well as schemes proposed by us, based on previously conducted studies to determine the sensitivity of isolated microflora to antibacterial drugs (aerosol treatment indoors with iodotriethyleneglycol solution with intramuscular administration of the drug ”Marfloxacin“) and phytobiotics (aerosol treatment indoors with Hypericum perforatum wort extract with intramuscular administration of the drug ”Marfloxacin“) were presented. Black-and-white calves, aged 1–3 months, mixed sex, with clinical signs of acute catarrhal bronchopneumonia were studied. The sick animals were divided into three experimental groups using the envelope method: 1O — experimental 1, n = 20; 2O — experimental group 2, n = 20 and 3O — experimental group 3, n = 20 and placed in separate isolators. During the treatment of animals in group 1O, the general clinical improvement occurred only on the 9.25 ± 0.91 day, while six cases of complications occurred, and two animals died. The treatment of calves in group 2O was accompanied by the general clinical improvement 2.05 days earlier, compared with group 1O, and all animals recovered. Therapy in group 3O contributed to the general clinical improvement already on the 4.90 ± 0.64 day, which is 47.0% earlier compared with the indicators of group 1O, and all 20 calves also recovered. The study of the processes of lipid peroxidation and antioxidant protection in blood plasma of experimental calves in the dynamics of treatment confirmed the best result in 3O group, which was accompanied by a significant decrease in LPO products and an increase in AOS indicators, which already on the 7th day of observation approached the physiological norm.
Copyright (c) 2025 Kulikov E.V., Sotnikova E.D., Rodionova N.Y., Prozorovskiy I.E., Shepeleva K.V., Rudenko P.A. https://creativecommons.org/licenses/by-nc/4.0
Influence of complex therapy on amino acid composition of blood in calves with gastrointestinal tract diseases
Significant changes in the amino acid composition of the blood were found in calves with dyspepsia. Amino acid analysis showed a heavy decrease in the levels of specific non-essential and essential amino acids in calves with dyspepsia. The aim of the study was to evaluate the effect of fermented colostrum on the amino acid composition of blood during the treatment of calves with dyspepsia. Three groups of 10 day-old calves were formed for the study. The first group of animals (the control group) was fed regular colostrum without fermentation for 7 days. The second and third groups (experimental) were calves with diarrhea syndrome. The second group of calves was administered 2 ml of Flunex intramuscularly, Roncoleukin 1500 IU/kg intravenously, and Thymogen was administered to calves intramuscularly at a dose of 100 mcg per animal on the 1st and 20th days of life. A full dose of milk was given in the evening milk feeding. The third group of calves underwent the same drug therapy. But in the morning and evening milk feedings they were given a full dose of colostrum fermented by Productiv Acid SE. The use of Flunex, Roncoleukin, Thymogen for the treatment of the disease does not contribute to the full restoration of the amino acid composition of the blood. After treatment, the content of specific essential amino acids did not reach the levels of healthy animals. The total amount of amino acids was also below the control values. Adding fermented colostrum to the diet of calves that were undergoing treatment contributes to the restoration of the amino acid composition of the blood. The content of specific essential amino acids slightly differed from the control values in this group animals. The amount of non-essential amino acids increased significantly and almost reached the control values.
Copyright (c) 2025 Pudovkin N.A., Apieva E.Z., Smutnev P.V. https://creativecommons.org/licenses/by-nc/4.0
The influence of climatic factors on sunflower ontogenesis in conditions of the Orenburg region
The effect of the variability of certain climatic parameters during the growing season on the cultivation of sunflower (Helianthus annuus L.) in the Orenburg region was studied. It was shown that the growth and development of Helianthus annuus L. are significantly influenced by both humidity and temperature, but the amount of these parameters changes over time due to the ongoing climate change. In this regard, one of the principles of agronomy is to identify relationships between the phases of phenological development in the context of changing weather conditions. It allows to assess the measures aimed at adapting sunflower to a specific cultivation zone. Timely implementation of these measures will enable us to manage the susceptibility of the agricultural sector and predict crop yields. The present study is based on registration of eight phenological phases from sowing to harvesting for three years and assessment of the influence of weather factors of a particular season on both ontogenesis of the crop in general and duration of each phenological phase in particular. According to the results of observations, it was found that the duration of Helianthus annuus L. cv. ‘Poseidon 625’ vegetation in climatic conditions of Orenburg region was on average 99 days and is determined by the timing from the formation of the first and second pairs of true leaves to seed formation. The phenological periods requiring maximum moisture were determined — from sowing to germination and from flowering to seed filling (r = 0.9, the significance level is 90%). The data obtained during the study can be used by farmers and agronomists to determine the optimal timing of sowing oilseeds not only in Orenburg region, but also in each specific region engaged in their production.
Copyright (c) 2025 Nazarova N.M., Fedorova D.G. https://creativecommons.org/licenses/by-nc/4.0
Prospects of Serratia plymuthica strain 23B78/1 as a biocontrol agent for tomato protection
Tomato protection from diseases is necessary to obtain high yields of quality fruits. In protected soil conditions, tomatoes bear fruit for up to 265 days, while the fruits are harvested 1 or 2 times a week starting from 60 days after seed germination for early varieties and 100 days for late varieties. When growing tomatoes, especially during the fruiting period, it is optimal to use biological pest control agents, which, unlike chemical ones, are harmless to humans and do not accumulate in the fruits. Existing biological products are not effective enough against the entire range of tomato diseases. It is necessary to look for new strains of microorganisms. This paper presents the results of the study of strain 23B78/1 Serratia plymuthica with the aim of exploring the prospects for its use as a biocontrol agent. Pesticides based on this species are not registered in the Russian Federation. Species identification was determined by 16S gene sequence analysis and biochemical profiling. Antagonistic activity against phytopathogenic fungi was assessed in vitro using double culture method. Phytotoxicity testing was carried out on germinating seeds of tomato. Evaluation of antagonistic effect revealed effectiveness against the following phytopathogenic fungi: Alternaria solani, Botrytis cinerea, Colletotrichum truncatum, Fusarium citri, F. incarnatum, F. duofalcatisporum, F. incarnatum, F. oxysporum, Globisporangium ultimum, Sclerotinia sclerotiorum. The maximum antagonistic effect was observed during paired fusion with fungus B. cinerea, mycelium of which grew strictly in the opposite direction from the bacterium. Germination of tomato seeds in presence of strain 23B78/1 did not reveal any inhibitory effect on seed germination and development of young tomato plants. The conducted research shows that the strain Serratia plymuthica 23B78/1 is promising for creation of biofungicide for protecting tomato plants.
Copyright (c) 2025 Platonov V.A., Njoya M.B., Elansky A.S., Skokov D.N., Elansky S.N., Chudinova E.M. https://creativecommons.org/licenses/by-nc/4.0
The use of microbiological agents in potato growing in the zone affected by metallurgical production
The efficiency of using microbial agents for growing potatoes in the zone of influence of metallurgical production in the conditions of the Southern Baikal region was assessed. The studies were carried out at three sites near a large industrial aluminum enterprise in the city of Shelekhov in 2015-2016. Microbial agents of Japanese (EM series) and Russian production (Baikal EM1, Fitosporin) were used in the study. It was revealed that the effectiveness of Fitosporin and EM preparations depended to a greater extent on soil’s pH and weather conditions. Thus, growing potatoes on slightly acidic soils using EM agents caused a stable increase in yield by 14-22%, and on alkaline soils, increasing yields were recorded in less than 50% of cases. At the same time, using Fitosporin-M bioagent on alkaline soils resulted in increasing potato yield by 13…22%. Using this bioagent on slightly acidic soils increased yield (by 26%) only in 2016. The results obtained indicate the prospects of further research on the use of biological products in the cultivation of agricultural crops in contaminated areas.
Copyright (c) 2025 Ivanova S.V., Ryabchikova I.A. https://creativecommons.org/licenses/by-nc/4.0
Study of optimal method of Heterodera glycines sample preparation for molecular diagnostics
In this study, it is optimized by the methods of preparing samples for the release of genetic material (DNA) from soybean cyst-forming nematode Heterodera glycines , which is necessary for accurate identification. Various modifications of ready-made reagent kits from Russian and foreign manufacturers were tested: DNA-Extrane-2, FitoSorb, TsytoSorb, ORB-GMO-A by Sintol LLC (Russia), Proba-GS, Proba-NK by AgroDiagnostika LLC, and QIAamp DNA Mini Kit by Qiagen (Germany). By testing the PCR method «In real time», the sensitivity of Heterodera glycines total DNA release was tested using a test system consisting of forward and reverse primers and a modified probe: SCNrtF (5’-AAATTCCAGGCCGCTATCTC-3’), SCNrtR (5’CGTGGACTGAACTGAACTGGACAAAG-3’), SCNrtP (5’-FAM/-TGGGCTGGGTGCTTCTAGAACTTTT-/ BHQ-1/-3’).
Copyright (c) 2025 Ivanov A.V., Bondarenko G.N. https://creativecommons.org/licenses/by-nc/4.0
Monitoring of the erythrocyte antigen structure in the gene pool herd of Red Gorbatov cattle
Immunogenetic analysis is of practical interest, allowing us to establish not only the relationship between animals, but also the degree of genetic influence of individual stud bulls on the entire gene pool of the population. While preserving the gene pool, the study of the frequency distribution of blood groups, as well as their changes over time, allow us to judge the processes occurring in closed populations. The research was carried out in the gene pool farm of the Red Gorbatov breed. The brood stock has 8 systems of blood groups. In 2007, 45 antigens were detected in the herd, in 2009 there was a decline in diversity to 38 antigens, but in 2010 their number was restored to 46, by 2017 the total number of antigens had not changed significantly — 47, and in 2022 again 46. The most numerous is EAB system, from 21 to 28 antigens were established in this system in different years. The structure of antigens is unstable, some antigens that were widespread in 2007 (Y2 and C2) decreased to 14…32 % in 2017. New antigens were established — O3, O4, Ox, and A’1. The loss of individual antigens may indirectly indicate the loss of other alleles that have economic importance; therefore, animals with rare antigens should be used in reproduction to a greater extent. The stud bulls whose seed stock is available at that moment have different antigenic composition: from 3 to 17 antigens in different systems. The use of a limited number of bulls can lead to the drift of individual genes.
Copyright (c) 2025 Rudenko O.V. https://creativecommons.org/licenses/by-nc/4.0
Modern approaches to the prevention of actinobacillus pleuropneumonia in pigs
Information about the current situation with actinobacillus pleuropneumonia (APP) in the Russian Federation is quite limited, which makes it difficult to assess the effectiveness of current preventive measures and develop new strategies. The diversity and effectiveness of vaccines play an important role. The main aim of the study is a scientific review of modern approaches to the APP prevention in pigs and prospects for the development of a vaccine that provides comprehensive protection against all known serotypes of this pathogen. The methods of systematization, critical analysis, and problem review are used. Over the years, various vaccines have been developed to combat this disease. Inactivated bacterial vaccines provide a strong immune response, but their effectiveness may vary. Based on the outer membrane proteins and three Apx toxoids, subunit vaccines are able to provide protection regardless of the APP serotype. DNA vaccines show promising results but require further research. Live attenuated vaccines contain weakened microorganisms and stimulate strong immunity, but their use also has its own difficulties. Despite all the successes achieved, at the moment there is no reliable and commercially profitable vaccine that would protect against all known serotypes of A . pleuropneumoniae and prevent the carriage and transmission of the disease. The problem of specific prevention of APP requires an integrated approach, including further scientific research, the development of new technologies and innovative methods of vaccination. Scientists increasingly focus on research in the field of oral and nasal vaccines. One of the important advantages is cost-effectiveness and safety during production, as well as convenience and safety of vaccine application. Oral and nasal vaccines can significantly simplify the immunization process, especially in the context of mass vaccination. Studies of vaccines based on external membrane vesicles and transgenic plants show promising results.
Copyright (c) 2025 Pimenov N.V., Shashkova A.A., Kruglov A.A. https://creativecommons.org/licenses/by-nc/4.0
Epidemiological characteristics of dogs with dental calculus: 392 cases (2012-2020)
Dental calculus is a local irritant to adjacent soft tissues, causing an inflammatory response that contributes to the development of periodontal diseases. Mineralized plaque contains bacterial toxins, which can lead to systemic infections. A retrospective analysis of the oral cavity condition of 392 dogs with dental calculus admitted for a comprehensive dental examination and oral sanitation to the Dentalvet veterinary center in 2012-2020 was conducted. An analysis of epidemiological data was performed to determine the risk factors for dental calculus formation. The study showed that small breed dogs aged 1 to 5 years were included in highrisk group for periodontal diseases. Tables with data on dental plaque and calculus indices, age characteristics of dogs, number of dog groups by skull type and breed, photographs and intraoral X-rays were provided.
Copyright (c) 2025 Spirina A.S., Spirina O.A., Bychkov V.S., Ermakov A.M. https://creativecommons.org/licenses/by-nc/4.0
Immunological parameters in serous catarrhal inflammation of the udder in lactating cows
The aim of this study is to investigate the immunological parameters of blood in lactating cows with serous-catarrhal mastitis to identify the pathogenetic mechanisms of the disease. The research was carried out in autumn in one of the livestock farms of the Amur region. The object of the study was lactating cows of Holstein breed at 2-4 months of lactation. Groups of cows were formed by 10 animals in each: control (healthy) and experimental (sick with serous catarrhal mastitis). The inflammatory process in the mammary gland of lactating cows was caused by tissue damage and complicated by opportunistic microflora. Protein metabolism disorders (hypoalbuminemia, dysproteinemia) and signs of haemoconcentration (increased haematocrit and haemoglobin, erythrocytosis) were observed in sick animals. Conditionally pathogenic microflora and products of inflammation in the mammary gland stimulated cellular and humoral immunity.
Copyright (c) 2025 Ostyakova M.E., Kositsyna K.S., Irkhin V.K. https://creativecommons.org/licenses/by-nc/4.0