LC50 determination and intoxication symptoms of a new pyridine carboxamide pesticide Flonicamid on common carp Cyprinus carpio

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The present study was undertaken to evaluate the lethal toxicity and stress signs of Flonicamid toward juvenile common carp ( Cyprinus carpio ). The 96 h LC50 values, determined 43.17 mgL-1 by probit analysis in a semi-static system. LC50 24, 48 and 72 h were 47.54, 41.83 and 43.51 mgL-1, respectively. Behavioral changes include hyperexcitement, erratic swimming, dark coloration, loss of equilibrium and lethargy were observed with different intensities. Consequently, mortality rate, stress signs and behavioral changes observed in response to the Flonicamid are dependent to dose and time exposure.

About the authors

Melika Ghelichpour

University of Tehran; Center for International Scientific Studies & Collaboration (CISSC)

Author for correspondence.

Department of Aquatic Animal Health, Faculty of Veterinary Medicine

Tehran, Iran

Ali Taheri Mirghaed

University of Tehran; Center for International Scientific Studies & Collaboration (CISSC)


Department of Aquatic Animal Health, Faculty of Veterinary Medicine

Tehran, Iran

Amalia Perez Jimenez

University of Granada; University of Porto


Department of Zoology

Granada, Spain; Porto, Portugal


  1. Cho SR, Koo HN, Yoon C, Kim GH. Sublethal effects of Flonicamid and thiamethoxam on green peach aphid, Myzus persicae and feeding behavior analysis. Journal of the Korean Society for Applied Biological Chemistry. 2011; 54(6):889—898. doi: 10.1007/BF03253177
  2. Morita M, Ueda T, Yoneda T, Koyanagi T, Haga T. Flonicamid, a novel insecticide with a rapid inhibitory effect on aphid feeding. Pest Management Science: formerly Pesticide Science. 2007; 63(10):969—973. doi: 10.1002/ps.1423
  3. Pazhanisamy K, Indra N. Toxic effects of arsenic on protein content in the fish, Labeo rohita (Hamilton). Nature, Environment and Pollution Technology. 2007; 6(1):113—116.
  4. Mirghaed AT, Ghelichpour M. Determination of lethal concentrations of indoxacarb in fingerling Cyprinus carpio at two temperatures. International journal of aquatic biology. 2015; 3(5):339—345. doi: 10.22034/ijab.v3i5.113
  5. Sandahl JF, Baldwin DH, Jenkins JJ, Scholz NL. Comparative thresholds for acetylcholinesterase inhibition and behavioral impairment in coho salmon exposed to chlorpyrifos. Environmental Toxicology and Chemistry: An International Journal. 2005; 24(1):136—145. doi: 10.1897/04-195R.1
  6. Nwani CD, Nagpure NS, Kumar R, Kushwaha B, Kumar P, Lakra WS. Lethal concentration and toxicity stress of Carbosulfan, Glyphosate and Atrazine to freshwater air breathing fish Channa punctatus (Bloch). International Aquatic Research. 2010; 2:105—111.
  7. Mohapatra B, Rengarajan K. Manual on bioassays in the laboratory and their techniques. CMFRI special publication. 1995; 64:1—75.
  8. Jonsson CM, Toledo MCF. Acute toxicity of endosulfan to the fish Hyphessobrycon bifasciatus and Brachydanio rerio. Archives of Environmental Contamination and Toxicology. 1993; 24(2):151—155. Available from: doi: 10.1007/BF01141341
  9. Joshi A, Rege M. Acute toxicity of some pesticides and a few inorganic salts to the mosquito fish Gambusia affinis (Baird & Girard). Indian Journal of Experimental Biology. 1980; 18(4):435—437.
  10. Naqvi SM, Hawkins R. Toxicity of selected insecticides (Thiodan®, Security®, Spartan®, and Sevin®) to mosquitofish, Gambusia alfinis. Bulletin of Environmental Contamination and Toxicology. 1988; 40(5):779—784. doi: 10.1007/BF01697530
  11. Swarup PA, Rao DM, Murty A. Toxicity of endosulfan to the freshwater fish Cirrhinus mrigala. Bulletin of Environmental Contamination and Toxicology. 1981; 27(1):850—855. doi: 10.1007/BF01611106
  12. Hussein S, El-Nasser M, Ahmed S. Comparative studies on the effects of herbicide atrazine on freshwater fish Oreochromis niloticus and Chrysichthyes auratus at Assiut, Egypt. Bulletin of Environmental Contamination and Toxicology. 1996; 57(3):503—510. doi: 10.1007/s001289900218
  13. Pandey S, Kumar R, Sharma S, Nagpure N, Srivastava SK, Verma M. Acute toxicity bioassays of mercuric chloride and malathion on air-breathing fish Channa punctatus (Bloch). Ecotoxicology and Environmental Safety. 2005; 61(1):114—120. doi: 10.1016/ j.ecoenv.2004.08.004
  14. Chandra S. Toxic effect of Malathion on acetylcholinesterase activity of liver, brain and gills of freshwater catfish Heteropneutes fossilis. Environ. Conserv. 2008; 9:45—52.
  15. Taheri Mirghaed A, Ghelichpour M, Mirzargar SS, Joshaghani H, Ebrahimzadeh Mousavi H. Toxic effects of indoxacarb on gill and kidney histopathology and biochemical indicators in common carp (Cyprinus carpio). Aquaculture Research. 2018; 49(4):1616—1627. doi: 10.1111/are.13617
  16. Roy W, Sutherland I, Rodger H, Varma K. Tolerance of Atlantic salmon, Salmo salar L., and rainbow trout, Oncorhynchus mykiss (Walbaum), to emamectin benzoate, a new orally administered treatment for sea lice. Aquaculture. 2000; 184(1-2):19—29. doi: 10.1016/ S0044-8486(99)00307-5
  17. Nunes B, Gaio A, Carvalho F, Guilhermino L. Behaviour and biomarkers of oxidative stress in Gambusia holbrooki after acute exposure to widely used pharmaceuticals and a detergent. Ecotoxicology and Environmental Safety. 2008; 71(2):341—354. doi: 10.1016/ j.ecoenv.2007.12.006
  18. Backström T, Brännäs E, Nilsson J, Magnhagen C. Behaviour, physiology and carotenoid pigmentation in Arctic charr Salvelinus alpinus. Journal of Fish Biology. 2014; 84(1):1—9. doi: 10.1111/jfb.12240
  19. Sarikaya R, Yılmaz M. Investigation of acute toxicity and the effect of 2, 4-D (2, 4-dichlorophenoxyacetic acid) herbicide on the behavior of the common carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae). Chemosphere. 2003; 52(1):195—201. doi: 10.1016/ S0045-6535(03)00106-1
  20. Farah MA, Ateeq B, Ali MN, Sabir R, Ahmad W. Studies on lethal concentrations and toxicity stress of some xenobiotics on aquatic organisms. Chemosphere. 2004; 55(2):257— 265. doi: 10.1016/j.chemosphere.2003.10.063

Copyright (c) 2019 Ghelichpour M., Mirghaed A.T., Jimenez A.P.

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