Analysis of C-reactive protein levels in uterine fluid of dairy cows

The aim is to study changes in the concentration of C-reactive protein, which serves as an indicator of inflammatory processes in diagnostics, in the exudate from the uterine cavity of dairy animals after parturition. In the period from 2020 to 2024, in the Volgograd Region, scientific work was carried out on the basis of LLC ʺSP ʹDonskoyeʹʺ. The level of C-reactive protein in the fluids obtained after uterine lavage and in plasma samples was determined using enzyme-linked immunosorbent assay (ELISA) on a series of samples and a diagnostic kit for this biomarker. The studies in the laboratory were carried out according to the instructions provided by the manufacturer of the analytical equipment and the necessary reagents. The study revealed modifications in the content of C-reactive protein (CRP) both in blood plasma and in the uterine syringing solutions in cows throughout the experiment. In the uterine lavage solutions of healthy cows from the control series, the average CRP level reached 23.62 ± 8.94 ng/ml, whereas in the animals of the experimental group suffering from metritis this indicator was significantly higher and amounted to 67.26 ± 26.32 ng/ml. There is a spread in the concentrations of C-reactive protein in cows of the two groups, which indicates their different immune activity. The study aims to evaluate C-reactive protein as a potential indicator of inflammation during uterine healing after labor. Based on the results, it can be concluded that determining the concentration of this protein in uterine fluid may be an effective method for identifying inflammatory conditions in this organ.