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Hematological and biochemical parameters of bulls’ blood when using mink carcass hydrolyzate as a feed additive
- Authors: Denisenko V.N.1, Balykov V.A.1, Rogov R.V.1, Kruglova Y.S.1
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Affiliations:
- Scriabin Moscow State Academy of Veterinary Medicine and Biotechnology
- Issue: Vol 17, No 3 (2022)
- Pages: 373-381
- Section: Veterinary science
- URL: https://agrojournal.rudn.ru/agronomy/article/view/19804
- DOI: https://doi.org/10.22363/2312-797X-2022-17-3-373-381
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Abstract
To improve the efficiency of ruminal digestion, researchers have developed a variety of enzyme feed additives. We are also working to find feed additives that improve the nutrition of rumen microflora, that is, prebiotics. For this purpose, we have proposed an animal protein hydrolyzate. The paper presents data reflecting the dynamics of hematological and biochemical parameters of blood serum of calves at the age of 3-4 months when hydrolyzed mink carcasses are included in the diet at doses of 5, 10 and 20 grams per head per day for 30 days. It was shown that after the completion of the experiment in animals treated with hydrolyzate at doses of 10 and 20 grams per head, there was a significant decrease in the content of leukocytes within the reference values and an increase in the percentage of lymphocytes in the leukogram. The change in the biochemical parameters of the groups of experimental bulls was characterized by a dose-dependent statistically significant increase in the concentration of protein, urea and reserve alkalinity. The concentration of total bilirubin, ALT, LDH significantly decreased, AST- increased. The results obtained indicate an increase in the immune reactivity of the animal organism with the use of the feed additive.
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Table 1. The results of a clinical blood test of control and experimental bulls
Indicators | Reference values | Control | 5 g/head | 10 g/head | 20 g/head | ||||
Before | After | Before | After | Before | After | Before | After | ||
Erythrocytes, ×1012/l | 5.0—7.5 | 8.13±0.70 | 8.31±0.72 | 7.61±0.44 | 7.60±0.99 | 7.32±0.48 | 7.82±0.50 | 7.47±0.50 | 7.52±0.32 |
Hematocrit,% | 28—38 | 45.83±1.90 | 46.35±0.79 | 45.83±1.90 | 46.35±0.79 | 45.78±1.90 | 46.42±1.54 | 45.90±4.15 | 46.98±1.55 |
Hemoglobin, g/l | 90—120 | 133.78±2.50 | 134.90±1.99 | 135.28±4.15 | 137.60±2.19 | 136.67±2.94 | 137.83±2.32 | 135.17±2.84 | 135.90±12.49 |
ESR, mm/h | 1—3 | 1.00±0.00 | 1.00±0.00 | 1.00±0.00 | 1.00±0.00 | 1.00±0.00 | 1.33±0.52 | 1.50±0.84 | 1.17±0.41 |
Leukocytes, ×109/l | 5.0—10.0 | 12.53±3.80 | 12.36±2.57 | 11.75±2.56 | 11.94±1.96 | 12.01±1.79 | 7.85±1.89* | 11.82±2.20 | 8.78±1.28* |
Stick. neutrophils,% | 2—5 | 1.17±0.41 | 1.50±0.55 | 1.17±0.41 | 1.50±0.55 | 1.00±0.00 | 1.00±0.00 | 1.50±0.55 | 1.50±0.55 |
Segment. neutrophils,% | 25—50 | 53.17±11.65 | 52.83±11.63 | 46.00±5.55 | 46.67±7.17 | 40.00±5.97 | 37.50±10.03 | 38.50±8.87 | 38.50±8.92 |
Eosinophils,% | 3—8 | 2.50±1.64 | 2.50±1.38 | 2.50±1.64 | 2.50±1.38 | 2.50±1.87 | 3.00±1.79 | 3.00±1.67 | 2.83±1.33 |
Monocytes,% | 3—10 | 3.00±1.90 | 3.00±1.67 | 3.00±1.90 | 3.00±1.67 | 2.50±1.76 | 2.17±1.60 | 2.67±1.37 | 2,83±1.47 |
Lymphocytes,% | 40—70 | 40.17±9.89 | 40.17±9.89 | 44.17±4.83 | 44.67±5.92 | 40.67±10.21 | 56.00±8.88* | 44.33±2.73 | 55.83±8.57* |
Platelets, ×109/l | 269—700 | 323.50±57.11 | 323.50±57.11 | 306.50±27.22 | 307.33±32.93 | 258.67±28.27 | 265.33±20.38 | 268.17±11.62 | 276.17±8.13 |
*Statistically significant changes in indicators compared to control at p < 0.05.
Тable 2. Biochemical parameters of control and experimental bulls
Indicators | Control | 5 g/head | 10 g/head | 20 g/head | ||||
Before | After | Before | After | Before | After | Before | After | |
Total protein, g/l | 69.14±7.15 | 66.98±13.43 | 66.38±6.63 | 71.40±7.27 | 69.42±10.91 | 84.60±2.35* | 69.60±4.18 | 82.70±4.81* |
Albumin, g/l | 35.36±2.62 | 36.14±1.84 | 34.04±1.46 | 34.72±2.48 | 34.68±1.67 | 37.00±1.57 | 34.18±6.91 | 35.30±3.59 |
Glucose, mmol/l | 1.92±0.51 | 1.74±0.28 | 1.94±0.34 | 2.04±0.21 | 1.38±0.22 | 1.44±0.34 | 1.34±0.52 | 1.54±0.18 |
Total bilirubin, µmol/l | 7.86±0.90 | 7.72±0.38 | 7.04±1.04 | 6.60±0.72* | 7.08±3.28 | 3.20±1.23* | 7.98±3.47 | 2.72±0.86* |
Aspartateaminotransferase, U/l | 24.80±3.27 | 27.40±2.88 | 23.02±4.30 | 42.00±9.35* | 24.78±4.55 | 82.40±4.93* | 24.68±2.54 | 110.00±11.90* |
Alanineaminotransferase, U/l | 105.02±4.05 | 96.90±9.13 | 106.00±12.63 | 65.60±4.72* | 107.32±44.15 | 20.16±6.35* | 101.53±26.96 | 16.44±4.79* |
Lactatedehydrogenase, U/l | 305.14±16.80 | 305.26±5.80 | 311.84±7.97 | 313.00±10.62 | 316.40±11.26 | 236.66±8.95* | 332.20±23.22 | 249.32±23.84* |
Urea, mmol/l | 3.74±0.54 | 3.55±0.43 | 3.38±1.54 | 3.23±1.68 | 3.01±0.88 | 3.96±0.72 | 3.17±0.61 | 4.19±0.76* |
Creatinine, µmol/l | 67.98±9.55 | 75.38±21.36 | 70.64±25.12 | 58.92±16.24 | 81.24±31.49 | 84.04±13.32 | 80.38±19.83 | 71.46±7.53 |
Calcium, mmol/l | 2.34±0.23 | 2.26±0.23 | 2.54±0.41 | 2.36±0.23 | 3.84±3.29 | 2.86±1.65 | 3.70±2.66 | 3.30±1.48 |
Phosphorus, mmol/l | 0.94±0.17 | 0.94±0.21 | 0.98±0.08 | 1.06±0.09 | 0.96±0.13 | 0.87±0.22 | 1.09±0.06 | 1.14±0.14 |
Carotene, mg/% | 0.44±0.04 | 0.41±0.07 | 0.75±0.11 | 0.51±0.20* | 0.63±0.16 | 0.45±0.05* | 0.53±0.10 | 0.39±0.08* |
Reserve alkalinity, mg/% | 300.00±64.81 | 306.00±68.41 | 308.00±22.80 | 312.00±26.83 | 280.00±83.67 | 490.00±30.00* | 260.00±23.72 | 484.00±16.73* |
*Statistically significant changes in indicators in relation to control at p < 0.05.
About the authors
Viktor N. Denisenko
Scriabin Moscow State Academy of Veterinary Medicine and Biotechnology
Email: dvet@yandex.ru
ORCID iD: 0000-0003-4704-0774
Doctor of Veterinary Sciences, Professor, Department of Disease Diagnostics, Therapy, Obstetrics and Animal Reproduction
23 Akademika Skryabina st., Moscow, 109472, Russian FederationVitaly A. Balykov
Scriabin Moscow State Academy of Veterinary Medicine and Biotechnology
Email: ginza17@mail.ru
ORCID iD: 0000-0002-6689-4452
PhD student, Department of Disease Diagnostics, Therapy, Obstetrics and Animal Reproduction
23 Akademika Skryabina st., Moscow, 109472, Russian FederationRoman V. Rogov
Scriabin Moscow State Academy of Veterinary Medicine and Biotechnology
Email: r.v.rogov86@mail.ru
ORCID iD: 0000-0002-3010-5714
Candidate of Biological Sciences, Associate Professor, Department of Disease Diagnostics, Therapy, Obstetrics and Reproduction
23 Akademika Skryabina st., Moscow, 109472, Russian FederationYulia S. Kruglova
Scriabin Moscow State Academy of Veterinary Medicine and Biotechnology
Author for correspondence.
Email: y7272@mail.ru
ORCID iD: 0000-0003-2953-0745
Candidate of Veterinary Sciences, Associate Professor, Department of Disease Diagnostics, Therapy, Obstetrics and Reproduction
23 Akademika Skryabina st., Moscow, 109472, Russian FederationReferences
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