ГИПЕРПОСМОЛЯРНЫЕ НАПОЛНИТЕЛИ СПЕРМЫ ПЕТУХОВ ПРИ ЕЕ КРИОКОНСЕРВАЦИИ

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Аннотация

Целью настоящей работы было исследование потенциального преимущества гиперосмолярного наполнителя в процессе замораживания-оттаивания спермы петухов. Цель заключалась в том, чтобы свести к минимуму количество внутриклеточной воды и уменьшить влияние внутриклеточного льда в процессе замораживания. В общей сложности у пяти 45-недельных коммерческих бройлеров кросса «Hubbard» были взяты образцы спермы в течение двухнедельного периода. Собранную сперму объединяли и делили на три равные части. Контрольный образец разбавляли трис-наполнителем без дополнительных добавок при 300 mOsm (контроль). Два других образца разбавляли трис-наполнителем при 300 мОм, содержащим витамин Е (Vit E), или с трис-наполнителем без витамина Е, но при осмолярности 450 mOsm (Hyper). После инкубации при 22 °С в течение 15 мин все образцы охлаждали и затем замораживали в жидком азоте. Для исследования влияния на различные параметры моторики был использован анализ спермы с использованием метода (CASA). После оттаивания наполнители с Hyperosmolar (Hyper) и витамином Е показали наилучшие значения с точки зрения сохранения подвижности сперматозоидов. Гиперосмолярный наполнитель (Hyper) показал особенно высокие значения в отношении скорости движения VSL и прогрессирующих подвижных сперматозоидов, известных как показатели качества спермы. Представленные результаты показали, что при использовании наполнителей спермы петухов при осмолярности, равной 450 mOsm, наблюдали существенное улучшение показателей сперматозоидов, которое было даже лучше, чем при использовании витамина Е. Положительное влияние, вероятно, связано с уменьшением внутриклеточного образования льда.

Об авторах

Mokrane M Iguerouada

University of Bejaia

Email: imokrane@gmail.com

Abdelaziz Norezzine

Ernst Institute for Animal Husbandry

Email: assissnor@gmail.com

Nazih Yacer Rebouh

Nemchinovka Moscow Research Institute of Agriculture

Email: n.rebouh@outlook.fr

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© Iguerouada M.M., Norezzine A., Rebouh N.Y., 2018

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