Quantitative determination of trypsin inhibitor as a breeding marker in maize varieties with different resistance to fungal diseases

Cover Page

Cite item


Determination of plant resistance to fungal pathogens is an important breeding component. Thus, the study of protein profiles from corn kernels (13 genotypes) revealed a constitutively pronounced 14 kDa protein, trypsin inhibitor (TI), which is present at a relatively high level of concentration in seven Aspergíllus flavus - resistant maize lines, but at low concentrations or is absent in six sensitive lines. The 14 kDa trypsin inhibitor (TI) also showed antifungal activity against other mycotoxicogenic species. In this regard, the task was to determine the content of TI in varieties of maize with known properties, resistance, or sensitivity to such fungal pathogens of maize as head smut, common smut, and Fusarium stalk rot. According to the data obtained, the content of TI varies in different varieties and can vary by 4 times. However, in disease-resistant varieties its content is increased, which may be the primary marker of resistance of the variety to fungal pathogens.

About the authors

Galina Vladimirovna Shekhvatova

Scientific Research Production Company Gamma

Author for correspondence.
Email: shgal06@gmail.com

Senior Researcher

Pushchino, Russian Federation

Viktor Vasilievich Ashin

Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS

Email: shgal06@gmail.com

Candidate of Biological sciences, Junior Researcher, Laboratory of Adaptation of Microorganisms

Pushchino, Russian Federation

Elena Fedorovna Sotchenko

Russian Research Institute of Maize

Email: shgal06@gmail.com

Candidate of Biological sciences, leading researcher, Department of Selection for immunity

Pyatigorsk, Russian Federation


  1. Chen ZY, Brown RL, Lax AR, Guo BZ, Cleveland TE, Russin JS. Resistance to Aspergillus flavus in corn kernels is associated with a 14-kDa protein. Phytopathology. 1998; 88(4):276- 281. doi: 10.1094/PHYTO.1998.88.4.276
  2. Chen ZY, Brown RL, Lax AR, Cleveland TE, Russin JS. Inhibition of plant-pathogenic fungi by a corn trypsin inhibitor overexpressed in Escherichia coli. Appl Environ Microbiol. 1999; 65(3):1320-1324.
  3. Selitrennikoff CP. Antifungal proteins. Appl Environ Microbiol, 2001; 67(7):2883-2894. doi: 10.1128/AEM.67.7.2883-2894.2001
  4. Zaynutdinova GF. Belkovye ingibitory ekzogennykh proteinaz v tkanyakh rastenii i ikh fiziologicheskaya rol' [Protein inhibitors of exogenous proteinases in plant tissues and their physiological role]. [Dissertation] Ufa; 2001. (In Russ).
  5. Chen ZY, Brown RL, Russin JS, Lax AR, Cleveland TE. A corn trypsin inhibitor with antifungal activity inhibits Aspergillus flavus α-amylase. Phytopathology. 1999; 89:902-907. doi: 10.1094/PHYTO.1999.89.10.902
  6. Hajare SS, Hajare SN, Sharma A. Screening of Indian corn varieties for aflatoxin resistance. BARC News Letter, Founder’s Day Special Issue. 2006; (273):218-230.
  7. Lei MG, Reeck GR. Combined use of trypsin-agarose affinity chromatography and reversed - phase high-performance liquid chromatography for the purification of single-chain protease inhibitor from corn seeds. Journal of Chromatography. 1986; 363(2):315-321. doi: 10.1016/S0021-9673(01)83751-1
  8. Chen ZY, Brown RL, Damann KE, Cleveland TE. Proteomics analysis of kernel embryo and endosperm proteins of corn genotypes resistant or susceptible to Aspergillus flavus infection. In: Robens J, Cary JW, Campbell BC. (eds.) Proceedings of the USDA-ARS Aflatoxin Elimination Workshop held at Yosemite. 2000. p. 88.
  9. Chen ZY, Brown RL, Damann KE, Cleveland TE. Identification of unique or elevated levels of kernel proteins in aflatoxin-resistant maize genotypes through proteome analysis. Phytopathology. 2002; 92(10):1084-1094. doi: 10.1094/PHYTO.2002.92.10.1084
  10. Mosolov VV, Valueva TA. Proteinase inhibitors and their function in plants: a review. Applied Biochemistry and Microbiology. 2005; 41(3):227-246. doi: 10.1007/s10438-005-0040-6

Copyright (c) 2019 Shekhvatova G.V., Ashin V.V., Sotchenko E.F.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies