A new primer set for amplification of ITS-rDNA in Ditylenchus destructor

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Abstract

A technique was developed for the identification of Ditylenchus destructor nematode belonging to the Ditylenchus genus, based on the use of different primers for polymerase chain reaction (PCR). Two universal ribosomal primers were amplified to the internal transcribed spacer region ITS-rDNA. The sequencing of PCR products confirmed the polymorphism between species. The primers were sensitive to generate a particular band of the correct size (300bp) from the DNA template of a single, separate D. destructor stage of development. Screening populations of D. destructor from Iran and the Russian Federation have tested the reliability of the primers, and the expected size of the band was produced for all test populations. Ditylenchus destructor closely related species have also been tested and no specific band was amplified. Such results showed that the primers currently developed are useful for quantifying the D. destructo r density in potato tuber.

About the authors

Niloufar Mahmoudi

Peoples’ Friendship University of Russia; All-Russian Plant Quarantine Centre

Author for correspondence.
Email: niloofarmahmoodi@ymail.com

PhD candidate, Department of Agro-Biotechnology, Agrarian and Technological Institute

Moscow, Russian Federation

Davoud K. Nejad

Semnan University

Email: Kartooli58@gmail.com

Assistant Professor of Forestry, Faculty of Desert Studies

Semnan, Iran

Fatemeh Shayanmehr

Tarbiat Modares University

Email: niloofarmahmoodi@ymail.com

PhD in Forestry, Natural Resources Faculty

Tehran, Iran

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Copyright (c) 2020 Mahmoudi N., Nejad D.K., Shayanmehr F.

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