Use of recombinant interleukin-2 in traumatic disease in veterinary medicine

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Abstract

Тhe article presents analysis of current information on the possibility of using recombinant interleukin-2 in traumatic disease in veterinary medicine. The analysis included publications from the following databases - P ubMed, MedLine, BIOSIS, ToxiNet, CANCERLIT, CINAHL, CISCOM, EMBASE, International Pharmaceutical Abstracts, and NAPRALERT, using 10 keywords and their combinations. It was shown that in trauma of any genesis, an immunodeficiency state occurs associated with an imbalance of Th1/Th2 cytokines. The use of recombinant interleukin-2 restores synthesis of endogenous interleukin-2, provides adequate targeted drug correction of immune dysfunctions, increasing the clinical and immunological effectiveness of therapeutic measures.

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Fig. 1. Absorption activity of neutrophilic granulocytes during co-cultivation with interleukin-2 in vitro, statistical significance of differences from the indicators on the 0th day of the study (Wilcoxon test). According to [5]

Fig. 2. Digestive capacity of neutrophilic granulocytes during co-cultivation with interleukin-2 in vitro, statistical significance of differences from the indicators on the 0th day of the study (Wilcoxon test). According to [5]

 

Table 1. Effect of rIL-2 on wound healing in vivo, according to [10]

№

Method of local treatment of wound 10 days after surgery

The size of the structures of the healing wound, μm (M±m)

Scab

Leukocyte shaft

Granulation tissue

Epithelium length

1

0.9 % NaCl solution: 1 ml applications on the wound + 1 ml s. c.; n = 10 rats (control)

213.5± 15.9

82.2 ± 6.2

750.6 ± 27.2

768.3 ± 53.1

2

Recombinant interleukin-2: 1 ml (50,000 IU) applications on the wound + 0.5 ml (20,000 IU/kg) s. c.; n = 12 rats

190.0 ± 16.2

53.1 ± 6.2*

1077.1 ±

34.6*

994.2 ± 43.1*

3

Recombinant interleukin-2: 1 ml (25,000 IU) applications on the wound + 0.5 ml (20,000 IU/kg) s. c.; n=12 rats

173.7 ± 14.9*

49.2 ± 5.2*

1118.7 ±

39.7*

972.1 ± 49.2*

Note: *Differences in relation to the data of series No. 1 are significant at p ≤ 0.001 (Student’s t-test was used).

 

Table 2. Effect of rIL-2 on reparative osteogenesis. According to [11]

№

Clinical indicators

Laboratory indicators

1.

Reducing endotoxicosis

Decreased erythrocyte sedimentation rate (ESR)

2.

rIL-2 erythropoietic activity

Increase in the number of erythrocytes by 23 % compared with the early postoperative period

3.

rIL-2 anti-inflammatory activity

Normalization of the ratio of various forms of neutrophilic granulocytes

4.

rIL-2 immunomodulating effect

Decreased content of IL-4 at an earlier date
Normalization of TNF-α level Maintaining IFN-γ level

5.

rIL-2 osteoinducing ability

Activation of osteoblasts, mineralization of the bone matrix
Normalization of the content of ionized and total calcium and inorganic phosphorus

6.

Functional limb restoration

Reducing the duration of fragment consolidation by 1.4 times

Scheme of pathogenetic therapy of rIL-2: subcutaneously at a dose of 20,000 IU/kg of body weight on the 1st, 3rd, 5th and 7th days of treatment.

×

About the authors

Oksana A. Gizinger

Peoples’ Friendship University of Russia

Author for correspondence.
Email: OGizinger@gmail.com
ORCID iD: 0000-0001-9302-0155

Doctor of Biological Sciences, Professor, Department of Microbiology and Virology, Medical Institute

8 Miklukho-Maklaya st., Moscow, 117049, Russian Federation

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1. Fig. 1. Absorption activity of neutrophilic granulocytes during co-cultivation with interleukin-2 in vitro, statistical significance of differences from the indicators on the 0th day of the study (Wilcoxon test). According to [5]

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2. Fig. 2. Digestive capacity of neutrophilic granulocytes during co-cultivation with interleukin-2 in vitro, statistical significance of differences from the indicators on the 0th day of the study (Wilcoxon test). According to [5]

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