Dynamics of morphofunctional parameters in experimental pseudomonosis of rabbits

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Abstract

Among the most frequently reported diseases of rabbits, the main place is occupied by diseases of bacterial etiology, the pathogens of which are characterized by multidrug resistance. The aim of the study was to analyze the dynamics of morphometric indicators during experimental infection of rabbits with Pseudomonas aeruginosa bacteria. When assessing colonization resistance of organs, the following indicators were taken into account: colonization index, ratio of the number of microorganisms isolated from nasal cavity washes, contents of cecum of clinically healthy and sick animals. Significant changes in morphological parameters (p ≤ 0.05) were revealed during the isolation of P. aeruginosa from blood, lymph nodes, spleen, liver, and kidneys of sick rabbits. Overgrowth of isolates producing adhesive antigens, hemolysins, bacteriocins should be considered as prognostic markers of connective tissue dysplasia. The initiation, development, and outcome of microbial overgrowth syndrome are mediated by morphofunctional changes in compensatory mechanisms of mucociliary clearance system and colonization resistance.

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Fig. 1. Dissemination of bacteria during intranasal infection with a culture of microorganisms P. aeruginosa, 1×108 CFU/ml
Source: made by the authors

Fig. 2. Rabbit thymus when infected with bacteria P. aeruginosa. Hematoxylin and eosin. Magnification: 10 × 20, H604 Trinocular Unico, USA
Source: made by the authors

Fig. 3. Rabbit liver when infected with bacteria P. aeruginosa. Hematoxylin and eo-sin. Magnification: 10 × 20, H604 Trinocular Unico, USA
Source: made by the authors

Fig. 4. Rabbit kidneys when infected with bacteria P. aeruginosa. Hematoxylin and eosin. Magnification: 10 × 20, H604 Trinocular Unico, USA
Source: made by the authors

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About the authors

Ekaterina M. Lenchenko

Russian Biotechnological University (RosBioTech)

Author for correspondence.
Email: lenchenko.ekaterina@yandex.ru
ORCID iD: 0000-0003-2576-2020

Doctor of Veterinary Sciences, Professor, Department of Veterinary Medicine, Institute of Veterinary Medicine, Veterinary and Sanitary Expertise and Agricultural Safety

11 Volokolamskoe highway, Moscow, 125080, Russian Federation

Galina S. Tolmacheva

Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences

Email: tgs2991@yandex.ru
ORCID iD: 0000-0001-9937-320X

Research engineer

26 Talalikhina st., Moscow, 109316, Russian Federation

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Supplementary files

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1. Fig. 1. Dissemination of bacteria during intranasal infection with a culture of microorganisms P. aeruginosa, 1×108 CFU/ml

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2. Fig. 2. Rabbit thymus when infected with bacteria P. aeruginosa. Hematoxylin and eosin. Magnification: 10 × 20, H604 Trinocular Unico, USA

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3. Fig. 3. Rabbit liver when infected with bacteria P. aeruginosa. Hematoxylin and eo-sin. Magnification: 10 × 20, H604 Trinocular Unico, USA

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4. Fig. 4. Rabbit kidneys when infected with bacteria P. aeruginosa. Hematoxylin and eosin. Magnification: 10 × 20, H604 Trinocular Unico, USA

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Copyright (c) 2023 Lenchenko E.M., Tolmacheva G.S.

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