Evaluation of hematological parameters in cats with intestinal lymphoma

Cover Page

Cite item

Abstract

Nowadays intestinal lymphoma is considered to be the most common tumor of alimentary tract in cats. The disease is characterized by severe course and poor response to chemotherapy. Since this tumor shares clinical and ultrasound characteristics with inflammatory bowel disease, the diagnosis is challenging and requires a variety of diagnostic modalities, including blood tests. Thus, the purpose of the research was to evaluate hematological parameters of cats with intestinal lymphoma. The diagnosis was confirmed using cytological and histological examinations. The objects of the research were cats of various breeds (20 animals in total), various sex and age groups. All animals underwent a clinical examination using standard methods, ultrasonographic examination of the abdominal cavity, and hematological studies. It was established that there was no correlation between disease stage and blood parameters. It was found that the first stage was not accompanied by changes in hematological parameters, the second stage was characterized by an increase in urea concentration (15.9 ± 7.9 mmol/l), the third stage was accompanied by an increase in ALT (115.6 ± 120.7 U/l) and AST (97 ± 96.1 U/l), fourth stage was characterized by an increase in leukocytes (19.4 ± 18.96), AST (134.6 ± 242), urea (18.5 ± 20.1) and creatinine (239.8 ± 257.1). The level of the blood parameters’ increase can be characterized as moderate and its origin is associated with progressive destruction of muscle mass, presence of concomitant diseases, but not with the tumor growth. The exception is an increased content of leukocytes in the blood, which can be explained by the body’s increased need for phagocytosis.

Full Text

Table 1
Indicators of general clinical blood test in cats with intestinal lymphoma at different stages

 Indicator

 Reference values

 Stage 1

 Stage 2

 Stage 3

 Stage 4

 Leukocytes, 109/L

 5.5…19.5

 9.7 ± 1.4

 12.5 ± 2.4

 12.45 ± 2.37

 19.4 ± 5.2

 Hemoglobin, g/L

 85.0…182.0

 138.0 ± 4.2

 112.0 ± 27.0

 119.0 ± 8.5

 118.2 ± 7.7

 Hematocrit, g/L

 26.0…61.0

 39.9 ± 1.6

 32.3 ± 9.4

 34.5 ± 2.2

 35.7 ± 2.4

 Erythrocytes, 1012/L

 4.60…12.18

 10.18 ± 0.61

 7.1 ± 1.2

 8.6 ± 0.6

 8.4 ± 0.78

 Mean corpuscular volume (MCV)

 38.0…54.0

 39.6 ± 2.3

 44.5 ± 5.8

 39.9 ± 1.01

 41.1 ± 2.8

 Mean corpuscular hemoglobin (MCH)

 11.8…19.7

 13.7 ± 0.8

 15.6 ± 1.2

 13.9 ± 0.48

 14.5 ± 0.51

 Mean corpuscular hemoglobin concentration (MCHC)

 33.0…360.0

 346.8 ± 6.3

 352.5 ± 18.5

 345.6 ± 5.84

 337.5 ± 5.2

 Neutrophils,%

 3.12…12.58

 17.9 ± 12.3

 9.6 ± 2.6

 7.97 ± 2.89

 9.16 ± 1.55

 Lymphocytes,%

 0.73…7.86

 2.9 ± 0.4

 2.17 ± 0.39

 1.9 ± 0.3

 2.26 ± 0.54

 Monocytes,%

 0.07…1.36

 0.31 ± 0.06

 0.42 ± 0.17

 0.27 ± 0.12

 0.54 ± 0.17

 Eosinophils,%

 0.06…1.93

 0.55 ± 0.12

 0.26 ± 0.04

 0.92 ± 0.49

 0.6 ± 0.22

 Basophils,%

 0.00…0.12

 0

 0

 0.02 ± 0.001

 0.01 ± 0.01

 Thrombocytes, thousand (PLT)

 100.0…730.0

 271.2 ± 28.5

 393.5 ± 149.5

 317.3 ± 135.01

 335.2 ± 36.24

Note. p = 0.05.
Source: completed by A.V. Goncharova, T.K. Atabaeva, V.A. Kostylev.

Table 2
Serum biochemistry parameters in cats with intestinal lymphoma at different stages

 Indicator

 Reference values

 Stage 1

 Stage 2

 Stage 3

 Stage 4

 ALT, U/L

 22.0…84.0

 61.0 ± 5.0

 67.5 ± 18.5

 115.6 ± 69.7

 60.3 ± 12.7

 AST, U/L

 18.0…51.0

 25.8 ± 1.9

 31.0 ± 5.0

 97.0 ± 68.0

 134.6 ± 82.6

 Total protein, g/L

 57.0…78.0

 71.8 ± 1.6

 69.5 ± 9.5

 63.3 ± 2.6

 62.15 ± 2.71

 Total bilirubin, μmol/L

 2.0…7.0

 3.0 ± 0.04

 2.0 ± 1.0

 5.0 ± 1.0

 5.65 ± 1.55

 Albumin, g/L

 23.0…35.0

 35.8 ± 0.9

 34.5 ± 2.5

 27.0 ± 1.0

 53.15 ± 27.81

 GGT, U/L

 1.0…10.0

 2.8 ± 1.8

 4.0 ± 1.0

 2.5 ± 1.5

 4.2 ± 1.1

 Urea, mmol/L

 6.28…11.71

 10.4 ± 0.4

 16.0 ± 5.6

 7.5 ± 0.39

 20.28 ± 6.08

 Creatinine, μmol/L

 71.0…159.0

 130.2 ± 5.6

 97.5 ± 14.5

 104.6 ± 8.2

 239.8 ± 78.3

Alkaline phosphatase, U/L

 9.0…53.0

 45.4 ± 3.1

 47.5 ± 3.5

 36.0 ± 4.5

 35.3 ± 5.9

 Phosphorus, mmol/L

 0.84…1.94

 1.34 ± 0.07

 1.4 ± 0.2

 1.62 ± 0.04

 1.62 ± 0.26

 Sodium, mmol/L

 143.0…165.0

 150.8 ± 1.9

 147.0 ± 5.0

 153.0 ± 3.0

 150.09 ± 1.69

 Potassium, mmol/L

 4.1…5.4

 3.88 ± 0.15

 3.9 ± 0.2

 5.05 ± 0.85

 3.8 ± 0.2

 Chloride, mmol/L

 107.0…122.0

 115.8 ± 1.6

 116.0 ± 6.0

 116.5 ± 0.5

 117.17 ± 1.42

 Glucose, mmol/L

 3.9…8.2

 4.79 ± 0.36

 7.45 ± 0.95

 6.03 ± 0.13

 6.4 ± 0.4

Note. p = 0.05.
Source: completed by A.V. Goncharova, T.K. Atabaeva, V.A. Kostylev.

Table 3
Calculations of nonparametric Kruskal — Wallis test  and asymptotic significance for four independent small samples

 Blood indicators

 H Kruskal — Wallis

 Asymptotic significance (two-sided)

 Basophils,%

 8.809

 0.032

 Albumin, g/L

 8.848

 0.031

 Urea, mmol/L

 7.891

 0.048

 Glucose, mmol/L

 9.842

 0.020

Source: completed by A.V. Goncharova, T.K. Atabaeva, V.A. Kostylev.

×

About the authors

Anna V. Goncharova

Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin

Email: annatrukhan@mail.ru
ORCID iD: 0000-0002-2142-2507
SPIN-code: 9362-5847

Doctor of Veterinary Sciences, Associate Professor, Professor of the Department of Veterinary Surgery

23 Akademika Skryabina st., Moscow, 109472, Russian Federation

Tatiana K. Atabaeva

Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin

Author for correspondence.
Email: tanya-atabaeva@yandex.ru
ORCID iD: 0009-0008-5749-7050

PhD student

23 Akademika Skryabina st., Moscow, 109472, Russian Federation

Vladislav A. Kostylev

Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin

Email: vetsurgery1@gmail.ru
ORCID iD: 0000-0003-1405-4213
SPIN-code: 9017-6331

Candidate of Veterinary Sciences, Associate Professor, Associate Professor of the Department of Veterinary Surgery

23 Akademika Skryabina st., Moscow, 109472, Russian Federation

References

  1. Atabaeva TK, Goncharova AV, Kostylev VA. Ultrasonographic prerequisites and substantiation of stages of intestinal lymphoma development in cats. Veterinariya. Zootekhniya i biotekhnologiya. 2023;2(12):31—37. (In Russ.). doi: 10.36871/vet.zoo.bio.202312203
  2. Atabaeva TK, Goncharova AV, Kostylev VA Analysis of anamnestic data and their role in occurrence and development of intestinal lymphoma in cats. Bulletin of Altai state agricultural university. 2023;(3):75—79. (In Russ.). doi: 10.53083/1996-4277-2023-221-3-75-79
  3. Gnezdilova LA, Panin AN, Pozyabin SV, Selina MV, Borunova SM. Diagnosis and prevention of infectious animal diseases based on monitoring. molecular diagnostics. and genomics. International Journal of Ecosystems and Ecology Science. 2022;12(3):459—470. doi: 10.31407/ijees12.358
  4. Mazurov VI, Krivolapov YA. Classification of lymphomas. Morphology. immunophenotype. molecular genetics of non-­Hodgkin’s lymphomas. Practical oncology. 2004;5(3):169—175. (In Russ.).
  5. Andreenko AA, Pozyabin SV. Features of diagnosis obstruction of the digestive tract in cats. Veterinariya. Zootekhniya i biotekhnologiya. 2016;(1):6—11. (In Russ.).
  6. Krivchikova YM. Estimation of selected biochemical blood parameters in patients with breast cancer undergoing chemotherapy with taxanes. Scientific result. Physiology Series. 2016;2(1):36—41. (In Russ.). doi: 10.18413/2409-0298-2016-2-1-36-41
  7. Vilmis DA. Hematological manifestations of paraneoplastic syndromes in dogs. International Journal of Veterinary Medicine. 2024;(1):381—391. (In Russ.). doi: 10.52419/issn2072-2419.2024.1.381
  8. Frantsyants EM, Bandovkina VA, Kushtova LB, Nikolaeva NV, Lysenko IB, Pushkareva TF. et al. Possibility of predicting the development of relapse in diffuse large B-cell lymphoma using indicators of a general blood test. Modern problems of science and education. 2021;(3):169. (In Russ.). doi: 10.17513/spno.30935
  9. Vail DM, Moore AS, Ogilvie GK, Volk LM. Feline lymphoma (145 cases): proliferation indices. cluster of differentiation 3 immunoreactivity and their association with prognosis in 90 cats. J Vet Intern Med. 1998;12(5):349—354. doi: 10.1111/j.1939-1676.1998.tb02134.x
  10. Barrs V, Beatty J. Feline alimentary lymphoma: 1. Classification. risk factors. clinical signs and non-invasive diagnostics. Journal of Feline Medicine and Surgery. 2012;14(3):182—190. doi: 10.1177/1098612X12439265
  11. Kiselow MA, Rassnick KM, McDonough SP, Goldstein RE, Simpson KW, Weinkle TK. et al. Outcome of cats with low-grade lymphocytic lymphoma: 41 cases (1995–2005). J Am Vet Med Assoc. 2008;232(3):405—410. doi: 10.2460/javma.232.3.405
  12. Vail DM, Thamm DH, Liptak JM. Small animal clinical oncology. 6th ed. Withrow & MacEwen’s; 2020.
  13. Sykes JE. Greene’s infectious diseases of the dog and cat. 5th ed. Elsevier Health Sciences; 2022.
  14. Lenkova NV, Wolf AV. Characteristics of functional and laboratory parameters in alimentary lymphoma in cats. International Journal of Veterinary Medicine. 2022;(4):241—246. (In Russ.). doi: 10.52419/issn2072-2419.2022.4.241
  15. Grigorieva II, Raskina TA, Letaeva MV, Malyshenko OS, Averkieva YV, Masenko VL. et al. Sarcopenia: pathogenesis and diagnosis. Fundamental and Clinical Medicine. 2019;4(4):105—116. (In Russ.). doi: 10.23946/2500-0764-2019-4-4-105116
  16. Ferreri AJM, Ernberg I, Copie-­Bergman C. Infectious agents and lymphoma development: molecular and clinical aspects. J Vet Intern Med. 2009;265(4):421—438. doi: 10.1111/j.1365-2796.2009.02083.x
  17. Louwerens M, London CA, Pedersen NC, Lyons LA. Feline lymphoma in the post-feline leukemia virus era. J Vet Intern Med. 2005;19(3):329—335. doi: 10.1892/0891-6640(2005)19[329: flitpl]2.0.co;2
  18. Stein TJ, Pellin M, Steinberg H, Chun R. Treatment of feline gastrointestinal small-cell lymphoma with chlorambucil and glucocorticoids. J Am Anim Host Assoc. 2010;46(6):413—417. doi: 10.5326/0460413

Copyright (c) 2024 Goncharova A.V., Atabaeva T.K., Kostylev V.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies