Biological efficacy of chitosan nanoparticles and black poplar buds ethanolic extract against potato dry rot

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Abstract

The purpose of the recent study was to evaluate the effectiveness of chitosan nanoparticles (CSNP) and black poplar buds extract (BpE) to protect potato tubers from post-harvest Fusarium dry rot. A high virulent Fusarium isolate was recovered from natural infected tubers in Astrakhan region and subjected to molecular identification using ITS rDNA gene and identified as Fusarium sumbucinum . CSNP were successfully prepared by ionic gelation method with size ranged from 79.4 to 186.4 nm as observed by scanning electron microscopy (SEM), and a positive zeta potential of 53.1 mv. CSNP at a concentration of 2 g/l, and BpE at a concentration of 40 g/l completely inhibited Fusarium fungal growth on PDA medium in vitro . CSNP at a rate of 20 g/t and BpE at a rate of 400 g/t significantly reduced the incidence of dry rot under natural infection during 8 months of storage by 54.7 and 58.5% and reduced the disease index by 65.7 and 72.3%, respectively, and there was not a significant difference between the two treatments. These results demonstrate the potential of CSNP and BpE for protection of potato tubers against the fungal postharvest decay.

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Elsayed M. Zeitar

Damanhour University; Astrakhan State University

Author for correspondence.
Email: elsayedzeitar@gmail.com
ORCID iD: 0000-0003-2106-556X
Scopus Author ID: 57221497255

PhD student, Department of Biotechnology, Aquaculture, Soil Science and Land Management, Faculty of Agro-biology, Astrakhan State University; Researcher, Plant Pathology Department, Faculty of Agriculture, Damanhour University

20a Tatishchev st., Astrakhan, 414056, Russian Federation; 27 Jalal quraytem square, Damanhour, 22516, Egypt

Liudmila T. Sukhenko

Astrakhan State University

Email: sukhenko@list.ru
ORCID iD: 0000-0001-5841-655X
SPIN-code: 8200-1324
ResearcherId: GWN-0323-2022

Doctor of Biological Sciences, Department of Biotechnology, Aquaculture, Soil Science and Land Management, Faculty of Biology

20a Tatishchev st., Astrakhan, 414056, Russian Federation

Sabah R. Mohammed

Damanhour University

Email: srabie106@gmail.com
ORCID iD: 0000-0002-1995-0730
Scopus Author ID: 57221498810

Candidate of Agricultural Sciences, Plant Protection Department, Faculty of Agriculture

27 Jalal quraytem square, Damanhour, 22516, Egypt

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Copyright (c) 2025 Zeitar E.M., Sukhenko L.T., Mohammed S.R.

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