Molecular identification of Ditylenchus destructor nematode with PCR Species-Specific primers in the Moscow region

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Abstract

Potato ( Solanum tuberosum L.) is one of the most vital food and industrial crop and Ditylenchus destructor is an influential pathogenic potato nematode and is quarantine pest in many states and territories. As a result, the polymerase chain reaction (PCR) protocol was optimized to identify Ditylenchus destructor reliably and rapidly. The species-specific internal transcribed spacer (ITS) was used as the primer of the D. destructor ribosomal DNA gene. Some populations of this species from the Moscow region in the Russian Federation were investigated through species-specific primer PCR. New sequence from ITS-rRNA was deposited in the GenBank under accession number MN122076. The current molecular technique is more appropriate to distinguishing of nematode species, since it is practical, fast and precise.

About the authors

Niloufar Mahmoudi

Peoples’ Friendship University of Russia (RUDN University); Russian Plant Quarantine Center (VNIIKR)

Author for correspondence.
Email: niloofarmahmoodi@ymail.com

PhD candidate, Department of Agro-Biotechnology, Agrarian and Technological Institute

Moscow, Russian Federation

Yousef Naserzadeh

Peoples’ Friendship University of Russia (RUDN University); Russian Plant Quarantine Center (VNIIKR)

Email: unaserzadeh@gmail.com

PhD candidate, Department of Agro-Biotechnology, Agrarian and Technological Institute

Moscow, Russian Federation

Elena N Pakina

Peoples’ Friendship University of Russia (RUDN University)

Email: e-pakina@yandex.ru

PhD, Associate Professor, Department of Agro-Biotechnology, Agrarian and Technological Institute

Moscow, Russian Federation

Liudmila A Limantceva

Russian Plant Quarantine Center (VNIIKR)

Email: Limantseva.ludmila@vniikr.ru

PhD, Senior scientist, Plant Quarantine Center

Moscow region, Russian Federation

Davoud Kartuli Nejad

Semnan University

Email: Kartooli58@gmail.com

Assistant Professor of Forestry, Faculty of Desert Studies

Semnan, Iran

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Copyright (c) 2019 Mahmoudi N., Naserzadeh Y., Pakina E.N., Limantceva L.A., Nejad D.K.

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