Specific Identification Method based on PCR for Drosophila melanogaster

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Abstract

D. melanogaster is one of the most harmful citrus fruit flies having a large number of host plants. The molecular diagnostic method has been created for identification the D. melanogaster from another non-quarantine species Drosophila spp. The proposed method for differentiation is to use the mitochondrial DNA cytochrome oxidase I gene region 709-bp. We amplified samples of DNA with primers Droso-S391 and Droso-A381 by D. melanogaster, D. suzukii, and D. Simulans collections in the laboratory samples from many countries and contrasted with sequences of other GenBank Drosophila taxa. The findings of a polymerase chain reaction (PCR) based on DNA sequence polymorphisms showed that these primers accurately identify the area of the gene as well as the unique primers of Drosophila melanogaster.

About the authors

Yousef Naserzadeh

Peoples’ Friendship University of Russia; All-Russian Plant Quarantine Centre

Author for correspondence.
Email: unaserzadeh@gmail.com

Researcher, Agro-Biotechnological Department, Agrarian and Technological Institute

Moscow, Russian Federation; Moscow region, Russian Federation

Elena N. Pakina

Peoples’ Friendship University of Russia

Email: e-pakina@yandex.ru

Associate Professor, Candidate of Biological Sciences, Agro-Biotechnological Department, Agrarian and Technological Institute

Moscow, Russian Federation

Abdorreza M. Nafchi

University Sains Malaysia

Email: amohammadi@usm.my

Professor

Penang, Malaysia

Anvar Sh. Gadzhikurbanov

Peoples’ Friendship University of Russia

Email: gadcgikurbanow@mail.ru

Agroengineering Department, Agrarian and Technological Institute

Moscow, Russian Federation

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Copyright (c) 2020 Naserzadeh Y., Pakina E.N., Nafchi A.M., Gadzhikurbanov A.S.

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