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The aim of the present work was undertaken to describe three cases of the bPAG concentration measure by three RIA systems during the post-partum period in non-pregnantcow. Three Holstein-Friesian cows of mixed age and parity were diagnosed as non-pregnant. Blood samples were removed from the coccygeal vessel into EDTA-coated tubes. Samples were collected every 2 days during a stabling period of two months in the absence of males. Plasma was obtained by centrifugation immediately after collection and was stored at -20 C until assay. Plasmatic PAG concentration was measured by radioim-munoassay technique with some modifications. In RIA-780 and RIA-809, there were high peak of PAG concentration that reached 2.56 ng/ml and 0.89 ng/ml, respectively. These peaks lasted longer than 3 days (two successive samples were positive). The other RIA systems gave the values of PAG concentrations below cut-off of pregnancy diagnosis (> 0.8 ng/ml) and remains always non-pregnant. Our data shows clearly that there is another source of glycoproteins expression (e.g. ovarian) apart from the placenta in cow. The present study could be a field for future larger studies on the same subject by exploring deeply other extra structures placental.

About the authors

Abdelhanine Ayad

University of Bejaia

Professor (DVM, MSc, PhD), Department of Biological Sciences of Environment, Faculty of Life and Nature Sciences, University of Bejaia. Bejaia, 06000, Algeria

Kamal Touati

University of Liege

Surgery for Large Animals, Faculty of Veterinary Medicine, University of Liege, Belgium Liege, 4000, Belgium


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Copyright (c) 2018 Ayad A., Touati K.

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