Morphological characteristics of testicular interstitial cell tumors in dogs

Cover Page

Cite item


The study presents an assessment of variability of histological structure, measurement, and comparison of size of the neoplasm obtained by ultrasonographic examination and pathological examination, as well as the morphometric dimensions of nuclei and cytoplasm of testicular interstitial cell tumors in dogs. The study involved 35 dogs with neoplasms of 46 testes, where 11 animals had interstitial cell tumors in both testes. Insignificant differences of the size of these neoplasms were revealed (p > 0.05) using ultrasonography and pathoanatomical measurement methods. Hence, it allows using both methods to assess the size of interstitial cell tumors. In the study, interstitial cell tumor was detected in both testes at once in 50 % of cases in dogs, however, this might be due to specific characteristics of the sample, and further research is required. In the course of scientific work, a morphological study showed the presence of variability in histological structure of interstitial cell tumors, which can lead to incorrect interpretation of the morphological picture and misdiagnosis, e. g. «adipocyte-like» morphology of interstitial cell tumors have morphological similarity to a benign neoplasm from adipose tissue - lipoma. In addition, there was an extremely pronounced difference in size of cytoplasm (from 23.6 to 148.4 µm; average 66.21 ± 22.42 µm) and nuclei (from 9 to 57.6 µm; average 23.19 ± 7.10 µm) in tumor cells. It proves the presence of pronounced anisocytosis and anisokariosis, which should indicate malignancy of the neoplasm, however, testicular interstitial cell tumors extremely rarely metastasize in practice and according to numerous studies.

Full Text

Mean clinical and pathological measurements showing that the differences between  the two measurement methods are not significant (p > 0.05)


Results of statistical processing of morphometric data obtained during the study  of testicular interstitial cell tumors in male dogs



Pathologoanatomic size

Clinical size









Standard deviation

























About the authors

Aleksey A. Gazin

Peoples’ Friendship University of Russia (RUDN University); Veterinary Oncology Scientific Center - ‘Biocontrol’ Veterinary Clinic

Author for correspondence.
ORCID iD: 0000-0001-7168-8744

postgraduate student, Department of Veterinary Medicine, Agrarian and Technological Institute, Peoples’ Friendship University of Russia; veterinarian-histologist, Department of Pathomorphological Diagnostics, Veterinary Oncological Research Center - ‘Biocontrol’ Veterinary Clinic

6 Miklukho-Maklaya st., Moscow, 117198, Russian Federation; 24/10 Kashirskoe highway, Moscow, 115522, Russian Federation

Yury A. Vatnikov

Peoples’ Friendship University of Russia (RUDN University)

ORCID iD: 0000-0003-0036-3402

Doctor of Veterinary Sciences, Professor, Director of the Department of Veterinary Medicine, Agrarian and Technological Institute

6 Miklukho-Maklaya st., Moscow, 117198, Russian Federation

Ekaterina V. Abramova

Veterinary Oncology Scientific Center - ‘Biocontrol’ Veterinary Clinic

ORCID iD: 0000-0001-8989-5071

Veterinarian-cytologist, Department of Pathomorphological Diagnostics

24/10 Kashirskoe highway, Moscow, 115522, Russian Federation


  1. Gazin AA, Lisitskaya KV, Vatnikov YA, Kornyushenkov EA. Incidence and differential diagnosis for canine testicular tumors. Bulletin of KSAU. 2021;(7):152-157. (In Russ.). doi: 10.36718/1819-4036-2021-7-152-157
  2. Gazin AA, Vatnikov YA, Sturov NV, Kulikov EV, Grishin V, Krotova EA, Razumova AA, Rodionova NY, Troshina NI, Byakhova VM, Lisitskaya KV. Canine testicular tumors: An 11-year retrospective study of 358 cases in Moscow Region, Russia. Veterinary World. 2022;15(2):483-487. doi: 10.14202/vetworld.2022.483-487
  3. Grieco V, Riccardi E, Greppi GF, Teruzzi F, Iermano V, Finazzi M. Canine testicular tumours: a study on 232 dogs. Journal of Comparative Pathology. 2008;138(2-3):86-89. doi: 10.1016/j.jcpa.2007.11.002
  4. Liao AT, Chu PY, Yeh LS, Lin CT, Liu CH. A 12-year retrospective study of canine testicular tumors. Journal of Veterinary Medical Science. 2009;71(7):919-923. doi: 10.1292/jvms.71.919
  5. Manuali E, Forte C, Porcellato I, Brachelente C, Sforna M, Pavone S, Ranciati S, Morgante R, Crescio IM, Ru G, Mechelli L. A five-year cohort study on testicular tumors from a population-based canine cancer registry in central Italy (Umbria). Preventive Veterinary Medicine. 2020;185:105201. doi: 10.1016/j.prevetmed.2020.105201
  6. Maxie MG. Jubb, Kennedy & Palmer’s Pathology of Domestic Animals: Volume 3. 6th ed. London: Elsevier health sciences; 2015.
  7. Meuten DJ. (ed.) Tumors in domestic animals. 5th ed. John Wiley & Sons; 2016.
  8. Nascimento HH, Santos AD, Prante AL, Lamego EC, Tondo LA, Flores MM, Fighera RA, Kommerset GD. Testicular tumors in 190 dogs: clinical, macroscopic and histopathological aspects. Pesquisa Veterinária Brasileira. 2020;40(7):525-535. doi: 10.1590/1678-5150-PVB-6615
  9. Vail DM, Thamm DH, Liptak J. (eds.) Withrow and MacEwen’s Small Animal Clinical Oncology. 6th ed. Elsevier Health Sciences; 2019.
  10. Kudo T, Kamiie J, Aihara N, Doi M, Sumi A, Omachi T, Shirota K. Malignant Leydig cell tumor in dogs: two cases and a review of the literature. Journal of Veterinary Diagnostic Investigation. 2019;31(4):557-561. doi: 10.1177/1040638719854791
  11. Nødtvedt A, Gamlem H, Gunnes G, Grotmol T, Indrebø A, Moe L. Breed differences in the proportional morbidity of testicular tumours and distribution of histopathologic types in a population-based canine cancer registry. Veterinary and Comparative Oncology. 2011;9(1)45-54. doi: 10.1111/j.1476-5829.2010.00231.x
  12. Togni A, Rütten M, Rohrer Bley C, Hurter K. Metastasized Leydig cell tumor in a dog. Schweiz Arch Tierheilkd. 2015;157(2):111-115. doi: 10.17236/sat00010
  13. Canadas A, Romão P, Gärtner F. Multiple cutaneous metastasis of a malignant Leydig cell tumour in a dog. Journal of Comparative Pathology. 2016;155(2-3):181-184. doi: 10.1016/j.jcpa.2016.05.012
  14. Orlandi R, Vallesi E, Boiti C, Polisca A, Bargellini P, Troisi A. Characterization of testicular tumor lesions in dogs by different ultrasound techniques. Animals. 2022;12(2):210. doi: 10.3390/ani12020210
  15. Doxsee AL, Yager JA, Best SJ, Foster RA. Extratesticular interstitial and Sertoli cell tumors in previously neutered dogs and cats: a report of 17 cases. The Canadian Veterinary Journal. 2006;47(8):763-766.

Supplementary files

Supplementary Files
1. Mean clinical and pathological measurements showing that the differences between the two measurement methods are not significant (p > 0.05)

Download (26KB)

Copyright (c) 2022 Gazin A.A., Vatnikov Y.A., Abramova E.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies