The efficiency genetic transformation by using retroviral vectors of chicken primordial germ cells

E K Tomgorova; Томгорова Евгения Константиновна; D V Beloglazov; Белоглазов Денис Валерьевич; N A Volkova; Волкова Наталья Александровна; A A Nikishov; Никишов Александр Алексеевич; N A Zinovieva; Зиновьева Наталия Анатольевна

doi:10.22363/2312-797X-2015-1-43-47

The efficiency genetic transformation by using retroviral vectors of chicken primordial germ cells

Authors: Tomgorova EK¹, Beloglazov DV¹, Volkova NA¹, Nikishov AA², Zinovieva NA¹
Affiliations:
1. All-Russian State Research Institute of Animal Breeding
2. Peoples’ Friendship University of Russia
Issue: No 1 (2015)
Pages: 43-47
Section: Articles
URL: https://agrojournal.rudn.ru/agronomy/article/view/1448
DOI: https://doi.org/10.22363/2312-797X-2015-1-43-47

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Abstract

In this article we studied the impact by using retroviral vectors for genetic transformation of chicken primordial germ cells IN VITRO and IN VIVO to produce transgenic chickens. By using of retroviral vector system it was shown that the efficiency genetic transformation of chicken embryonic gonads can be of up to 3.2%.