Association of A80V, R25C, AND Y7F leptin gene sites with productive longevity duration in Kholmogory cows

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Abstract

In modern dairy farming, the study of genetic factors affecting the productive longevity and milk productivity of cattle is relevant. The leptin gene, involved in the regulation of energy metabolism, is a promising marker for selection. However, the influence of its polymorphism complex genotypes (A80V, R25C, Y7F) on the productivity and longevity of animals has not been sufficiently studied, which determines the importance of this research. The aim of the study was to investigate the influence of the leptin gene complex genotypes at sites A80V, R25C, Y7F on the milk productivity indicators and the duration of economic use of Kholmogory cattle. The study was conducted on 207 Kholmogory breed cows, divided into groups with different complex genotypes. For statistical analysis, the number of animals in the compared groups was equalized: 13 heads per group for the study of lifetime milk productivity and 19 heads per group for the assessment of the duration of use. Genotyping was performed by PCR-RFLP, and statistical processing was carried out using non-parametric methods (Mann - Whitney U test). Lifetime daily milk yield, milk fat and protein content, age at culling, and lifetime milking days (LMD) were analyzed. It was revealed that the AVRCYY genotype is associated with the maximum daily milk yield (22.04 kg) and the highest number of LMD (2032 days), indicating its positive influence on productive longevity. The lowest indicators were noted for the AVCCYY genotype (194 LMD). Significant differences in milk fat content were found between groups AACCYY, AARCYY, AARRYY, and AVCCYY. The AACCYY genotype showed the highest protein content (3.23%), but the lowest milk yield. Differences in the structure of culling reasons were also identified: gynecological diseases prevailed in animals with the AVCCYY genotype combination, while limb diseases prevailed in animals with the AVRCYY complex genotype. The results of the study demonstrate the significance of a comprehensive analysis of leptin gene genotypes for dairy cattle selection. The AVRCYY genotype can be recommended as a marker for increased productive longevity.

About the authors

Natalya A. Khudyakova

Federal Research Center for Comprehensive Study of the Arctic named after Academician N.P. Laverov

Email: nata070707hudyakova@yandex.ru
SPIN-code: 0000-0003-1302-2965 SPIN-код: 3906-2286
Candidate of Agricultural Sciences, Senior Researcher, Laboratory of Innovative Technologies in the Agro-Industrial Complex 20 Nikolsky Prospekt, Arkhangelsk, 163000, Russian Federation

Irina S. Kozhevnikova

Federal Research Center for Comprehensive Study of the Arctic named after Academician N.P. Laverov; Northern (Arctic) Federal University named after M.V. Lomonosov

Email: kogevnikovais@yandex.ru
SPIN-code: 0000-0001-7194-9465 SPIN-код: 2441-2363
Candidate of Biological Sciences, Head of Laboratory, Laboratory of Innovative Technologies in the Agro-Industrial Complex, Federal Research Center for Comprehensive Study of the Arctic named after Academician N.P. Laverov; Associate Professor, Department of Human Biology and Biotechnical Systems, Northern (Arctic) Federal University named after M.V. Lomonosov 20 Nikolsky Prospekt, Arkhangelsk, 163000, Russian Federation; 17 Northern Dvina Embankment, Arkhangelsk, 163002, Russian Federation

Inga A. Klassen

Federal Research Center for Comprehensive Study of the Arctic named after Academician N.P. Laverov

Email: klassening@gmail.com
SPIN-code: 0000-0002-4421-6087 SPIN-код: 5343-4901
Junior Researcher, Laboratory of Innovative Technologies in the Agro-Industrial Complex 20 Nikolsky Prospekt, Arkhangelsk, 163000, Russian Federation

Aleksandra O. Stupina

Federal Research Center for Comprehensive Study of the Arctic named after Academician N.P. Laverov

Email: sashaatupina6@gmail.com
SPIN-code: 0000-0001-7664-3684 SPIN-код: 4712-2893
Junior Researcher, Laboratory of Innovative Technologies in the Agro-Industrial Complex 20 Nikolsky Prospekt, Arkhangelsk, 163000, Russian Federation

Maria S. Kalmykova

Federal Research Center for Comprehensive Study of the Arctic named after Academician N.P. Laverov

Email: kalmykova.m@edu.narfu.ru
SPIN-code: 0009-0000-0404-4631 SPIN-код: 3670-8226
Junior Researcher, Laboratory of Innovative Technologies in the Agro-Industrial Complex 20 Nikolsky Prospekt, Arkhangelsk, 163000, Russian Federation

References

  1. Часовщикова М.А., Козлов К.А. Продуктивное долголетие коров черно-пестрой породы в зависимости от возраста и живой массы при первом осеменении // Агропродовольственная политика России. 2021. № 4. С. 37-40. EDN: XNCIZG
  2. Wangchuk K., Wangdi J., Mindu M. Comparison and reliability of techniques to estimate live cattle body weight // Journal of Applied Animal Research. 2018. Vol. 46. № 1. P. 349-352. doi: 10.1080/09712119.2017.1302876
  3. Панков М.Н., Смолина В.С., Ступина А.О., Классен И.А., Спасский Е.А. Бета-казеин коровьего молока и его влияние на организм человека (обзор) // Журнал медико-биологических исследований. 2024. Т. 12. № 3. С. 411-418. doi: 10.37482/2687-1491-Z207 EDN: QWJTGF
  4. Ковалюк Н.В., Гырнец Е.А. Полиморфизм аллелей гена Lep как генетический маркер функционального долголетия крупного рогатого скота айрширской породы // Universum: химия и биология. 2016. № 6 (24). С. 1-6. URL: http://7universum.com/ru/nature/archive/item/3257 EDN: WAIRVB
  5. Худякова Н.А., Кожевникова И.С., Ступина А.О., Классен И.А., Кашин А.С. Динамика живой массы коров холмогорской породы по полиморфизму гена лептина // Аграрная наука Евро-Северо-Востока. 2025. Т. 26. № 1. С. 158-165. doi: 10.30766/2072-9081.2025.26.1.158-165 EDN: RPVEBM
  6. Комендант Т.М., Епишко О.А., Чебуранова Е.С. Оптимизация методики выявления полиморфизмов гена лептина (LEP), влияющего на продуктивное долголетие крупного рогатого скота // Сборник научных трудов Краснодарского научного центра по зоотехнии и ветеринарии. 2017. № 37. С. 99-105. EDN: YOZAZO
  7. Щеголев П.О., Сабетова К.Д., Чаицкий А.А., Сорокина А. Связь полиморфизма гена лептина (LEP) с хозяйственно полезными признаками крупного рогатого скота // Аграрный вестник Нечерноземья. 2021. № 1 (1). С. 25-32. doi: 10.52025/2712-8679_2021_01_25 EDN: HBJLBZ
  8. Гайнутдинова Э.Р., Сафина Н.Ю., Шакиров Ш.К., Варламова М.И. Влияние полиморфизма гена лептина (LEP) на молочную и мясную продуктивность коров-первотелок голштинской породы // Ученые записки Казанской государственной академии ветеринарной медицины им. Н.Э. Баумана. 2021. Т. 245. № 1. С. 24-28. doi: 10.31588/2413-4201-1883-245-1-24-28 EDN: ZRWOHH
  9. Ковалюк Н.В., Сацук В.Ф., Мачульская Е.В., Морковкина Н.А., Шахназарова Ю.Ю. Использование полиморфизма локуса LEP в селекции чернопестрого скота // Молочное и мясное скотоводство. 2017. № 3. С. 14-16. EDN: YTDPGR
  10. Komisarek J. Impact of LEP and LEPR gene polymorphisms on functional traits in Polish HolsteinFriesian cattle // Animal Science Papers and Reports. 2010. Vol. 28. P. 133-141.
  11. Szyda J., Morek-Kopec М., Komisarek J., Zarnecki A. Evaluation markers in selected genes for association with functional longevity of dairy cattle // BMC Genetics. 2011. Vol. 12. P. 3. doi: 10.1186/1471-2156-12-30 EDN: OAQCYN
  12. Ковалюк Н.В., Сацук В.Ф., Мачульская Е.В., Шахназарова Ю.Ю. Связь полиморфизма локусов R25C и A80V гена лептина с хозяйственно-ценными признаками у крупного рогатого скота голштинской породы // Сборник научных трудов СКНИИЖ. 2017. Т. 6. № 3. С. 16-21. EDN: VRCCIP
  13. Yoon D.H., Cho B.H., Park B.L., Choi Y.H., Cheong H.S., Lee H.K., Chung E.R., Cheong I.C., Shin H.D. Highly Polymorphic Bovine Leptin Gene // Journal of Animal Science. 2005. Vol. 18. № 11. P. 1548- 1551. doi: 10.5713/ajas.2005.1548
  14. Ковалюк Н.В., Сацук В.Ф., Волченко А.Е., Мачульская Е.В., Шахназарова Ю.Ю. Использование полиморфизма локуса лептина в селекции крупного рогатого скота айрширской породы // Молочное и мясное скотоводство. 2014. № 6. С. 13-15. EDN: SQJJJN
  15. Szyda J. Statistical modeling of candidate gene effects on milk production traits in dairy cattle // Journal of Dairy Science. 2007. Vol. 90. № 6. P. 2971-2979. doi: 10.3168/jds.2006-724

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Copyright (c) 2025 Khudyakova N.A., Kozhevnikova I.S., Klassen I.A., Stupina A.O., Kalmykova M.S.

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