The study of factors affecting the efficiency of somatic transgenesis in chickens

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Abstract


In this article we studied the impact of the use of retroviral vectors for genetic transformation of chicken oviduct cells in vivo to produce transgenic chickens bioreactors and investigated factors affecting the efficiency of local transgenesis in chickens. By using of retroviral vector system it was shown that with the efficiency of chickens oviduct cells transformation was at least 17,2 ± 3,1%.

Yu A Prytkov

All-Russian State Research Institute of Animal Breeding

Author for correspondence.
Email: prytkov@mail.ru

Центр биотехнологии и молекулярной диагностики

A N Vetokh

All-Russian State Research Institute of Animal Breeding

Email: anastezuya@mail.ru
Department of standardization, metrology and technology of the livestock products Peoples’ Friendship University of Russia Mikluho-Maklaya str., 8, Moscow, Russia, 117198; Department of standardization, metrology and technology of the livestock products Peoples’ Friendship University of Russia Mikluho-Maklaya str., 8, Moscow, Russia, 117198

N A Volkova

All-Russian State Research Institute of Animal Breeding

Email: natavolkova@inbox.ru

Центр биотехнологии и молекулярной диагностики

A A Nikishov

Peoples’ Friendship University of Russia

Email: a.nikishov@rudn.ru
Department of standardization, metrology and technology of the livestock products

N A Zinovieva

All-Russian State Research Institute of Animal Breeding

Email: n_zinovieva@mail.ru

Центр биотехнологии и молекулярной диагностики

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Copyright (c) 2014 Прытков Ю.А., Ветох А.Н., Волкова Н.А., Никишов А.А., Зиновьева Н.А.

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