The study of factors affecting the efficiency of somatic transgenesis in chickens

Yu A Prytkov; Прытков Юрий Александрович; A N Vetokh; Ветох Анастасия Николаевна; N A Volkova; Волкова Наталья Александровна; A A Nikishov; Никишов Александр Алексеевич; N A Zinovieva; Зиновьева Наталия Анатольевна

doi:10.22363/2312-797X-2014-3-49-54

The study of factors affecting the efficiency of somatic transgenesis in chickens

Authors: Prytkov Y.A¹, Vetokh AN¹, Volkova NA¹, Nikishov AA², Zinovieva NA¹
Affiliations:
1. All-Russian State Research Institute of Animal Breeding
2. Peoples’ Friendship University of Russia
Issue: No 3 (2014)
Pages: 49-54
Section: Articles
URL: https://agrojournal.rudn.ru/agronomy/article/view/1535
DOI: https://doi.org/10.22363/2312-797X-2014-3-49-54

Cite item

Full Text

Abstract
About the authors
References
Statistics

Abstract

In this article we studied the impact of the use of retroviral vectors for genetic transformation of chicken oviduct cells in vivo to produce transgenic chickens bioreactors and investigated factors affecting the efficiency of local transgenesis in chickens. By using of retroviral vector system it was shown that with the efficiency of chickens oviduct cells transformation was at least 17,2 ± 3,1%.

Keywords

chicken, transgenesis, oviduct, bioreactors, recombinant DNA

About the authors

Yu A Prytkov

All-Russian State Research Institute of Animal Breeding

Author for correspondence.
Email: prytkov@mail.ru

Центр биотехнологии и молекулярной диагностики

A N Vetokh

All-Russian State Research Institute of Animal Breeding

Email: anastezuya@mail.ru
Department of standardization, metrology and technology of the livestock products Peoples’ Friendship University of Russia Mikluho-Maklaya str., 8, Moscow, Russia, 117198; Department of standardization, metrology and technology of the livestock products Peoples’ Friendship University of Russia Mikluho-Maklaya str., 8, Moscow, Russia, 117198