Development diagnostic methods of bacterial blight of rice on based PCR

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Abstract


Primers identifying bacterial blight pathogen, without cross-reactions with closely related species were designed based on the analysis of the original and GeneBank DNA sequences of Xanthomonas oryzae pv. oryzae. Media YPGA containing cycloheximide 50 mg / l + cefazolin 30 mg / l + gentamicin 2 mg / l has been optimized for BIO-PCR. Sensitivity of the BIO-PCR analysis using original selective media up to 100 times more effective than direct PCR analysis. The sensitivity of the primers in BIO-PCR analysis with original selective media has achieved 50 CFU/ml.

M S Egorova

All-Russian Plant Quarantine Center

Author for correspondence.
Email: masha_0787@mail.ru

A N Ignatov

Peoples’ Friendship University of Russia

Email: an.ignatov@gmail.com
Department of botany, plant physiology and agrobiotechnology

E S Mazurin

All-Russian Plant Quarantine Center

Email: zarauh@mail.ru

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Copyright (c) 2014 Егорова М.С., Игнатов А.Н., Мазурин Е.С.

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