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Development diagnostic methods of bacterial blight of rice on based PCR
- Authors: Egorova M.S., Ignatov A.N., Mazurin E.S.
- Issue: No 2 (2014)
- Pages: 22-27
- Section: Articles
- URL: http://agrojournal.rudn.ru/agronomy/article/view/1625
- DOI: http://dx.doi.org/10.22363/2312-797X-2014-2-22-27
Abstract
Primers identifying bacterial blight pathogen, without cross-reactions with closely related species were designed based on the analysis of the original and GeneBank DNA sequences of Xanthomonas oryzae pv. oryzae. Media YPGA containing cycloheximide 50 mg / l + cefazolin 30 mg / l + gentamicin 2 mg / l has been optimized for BIO-PCR. Sensitivity of the BIO-PCR analysis using original selective media up to 100 times more effective than direct PCR analysis. The sensitivity of the primers in BIO-PCR analysis with original selective media has achieved 50 CFU/ml.
M S Egorova
All-Russian Plant Quarantine Center
Author for correspondence.
Email: masha_0787@mail.ru
A N Ignatov
Peoples’ Friendship University of Russia
Email: an.ignatov@gmail.com
Department of botany, plant physiology and agrobiotechnology
E S Mazurin
All-Russian Plant Quarantine Center
Email: zarauh@mail.ru
