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Effects of various pre-sowing treatments on invitro seed germination of Ceratoniasiliqua L.
- Authors: Duksi F.1
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Affiliations:
- RUDN University (Peoples’ Friendship University of Russia)
- Issue: Vol 17, No 2 (2022)
- Pages: 158-165
- Section: Crop production
- URL: https://agrojournal.rudn.ru/agronomy/article/view/19762
- DOI: https://doi.org/10.22363/2312-797X-2022-17-2-158-165
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Abstract
The organic dormancy of Ceratonia seeds is associated with their water resistance, which means that Carob suffers from difficulty in natural regeneration. Before planting Carob seeds must be treated in order to disturb mechanical dormancy. The main reasons for the decline in genus Ceratonia L. are anthropogenic impact on natural ecosystems, as well as the uses of Ceratonia for many goals. This problem is aggravated by the fact that seedlings of Ceratonia in nature are very rare because of organic dormancy, and the distribution of carob occurs mainly with the help of seeds. The aim of this work was to study the methods of pre-sowing treatment of dormant Carob seeds. This study was conducted on a wild Carob genotype grown in Syria. Four different pre-sowing treatments were the following: soaking in boiling distilled water (70 °С) for 10 min; soaking in boiling distilled water (70 °С) for 10 min + soaking in distilled water for 24 h; acid scarification with sulphuric acid (H2SO4); acid scarification with sulphuric acid (H2SO4) + soaking in distilled water for 24 h. We applied and examined for their effectiveness stimulation of Carob seed germination. The results showed that seeds treated with sulphuric acid (H2SO4) and then soaked in distilled water for 24 h was the most effective method increasing the germination percentage by 98 % compared to untreated seeds 5 %.
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Influence of treatments on seed germination
Treatment | Seed germination,% | First germination day | Last germination day | Germination period for all seeds, days |
Control (untreated seeds) | 5 | 10 | 56 | 46 |
Soaking in hot water (70 °C) for 10 minutes | 84 | 7 | 16 | 9 |
Soaking in hot water (70 °C) for 10 minutes + soaking in distilled water for 24 hours | 89 | 6 | 11 | 5 |
Scarification with concentrated sulfuric acid H2SO4 for 30 minutes | 91 | 4 | 8 | 4 |
Scarification with concentrated sulfuric acid H2SO4 for 30 minutes + soaking in distilled water for 24 hours | 98 | 3 | 7 | 4 |
Fig. 1. Soaking seeds in hot distilled water (70 °C) for 10 minutes: а — immediately after treatment; б — nine days after treatment
Fig. 2. Soaking seeds in hot distilled water (70 °C) for 10 minutes + soaking in distilled water for 24 hours: а — immediately after treatment; б — nine days after treatment
Fig. 3. Scarification seeds with H2SO4 for 30 minutes: а — immediately after treatment; б — nine days after treatment
Fig. 4. Scarification seeds with H2SO4 for 30 minutes + soaking in distilled water for 24 hours: а — immediately after treatment; б — nine days after treatment
About the authors
Fatima Duksi
RUDN University (Peoples’ Friendship University of Russia)
Author for correspondence.
Email: f.duksi@gmail.com
ORCID iD: 0000-0002-7353-7816
post-graduate student, Agrarian and Technological Institute
6 Miklukho-Maklaya st., Moscow, 117198, Russian FederationReferences
- Eldeeb GSS, Mosilhey SH. Roasting temperature impact on bioactive compounds and PAHs in Carob powder (Ceratonia siliqua L.). J Food Sci Technol. 2022;59:105-113. doi: 10.1007/s13197-021-04989-7
- Viruel J, Le Galliot N, Pironon S, Nieto Feliner G, Suc J, Lakhal-Mirleau F, Juin M, Selva M, Kharrat MBD, Ouahmane L, Malfa SL, Diadema K, Sanguin H, Médail F, Baumel A. A strong east - west Mediterranean divergence supports a new phylogeographic history of the carob tree (Ceratonia siliqua, Leguminosae) and multiple domestications from native populations. Journal of Biogeography. 2019;47(2):460-471. doi: 10.1111/jbi.13726
- Gregoriou G, Neophytou CM, Vasincu A, Gregoriou Y, Hadjipakkou H, Pinakoulaki E, Christodoulou MC, Ioannou GD, Stavrou IJ, Christou A, Kapnissi-Christodoulou CP, Aigner S, Stuppner H, Kakas A, Constantinou AI. Anti-cancer activity and phenolic content of extracts derived from cypriot carob (Ceratonia siliqua L.) pods using different solvents. Molecules. 2021;26(16):5017. doi: 10.3390/molecules26165017
- Pouresmaeil V, Haghighi S, Raeisalsadati AS, Neamati A, Homayouni-Tabrizi M. The anti-breast cancer effects of green-synthesized zinc oxide nanoparticles using carob extracts. Current Medicinal Chemistry - A nti- Cancer Agents. 2021;21(3):316-326. doi: 10.2174/1871520620666200721132522
- Rtibi K, Marzouki K, Salhi A, Sebai H. Dietary supplementation of carob and whey modulates gut morphology, hemato-biochemical indices, and antioxidant biomarkers in rabbits. Journal of Medicinal Food. 2021;24(10):1124-1133. doi: 10.1089/jmf.2020.0185
- Yuan, L, Wu Y, Qin Y, Yong H, Liu J. Recent advances in the preparation, characterization and applications of locust bean gum-based films. Journal of Renewable Materials. 2020;8(12):1565-1579. doi: 10.32604/ jrm.2020.014562
- Zannou O, Güclü G, Koca I, Selli S. Carob beans (Ceratonia siliqua L.): uses, health benefits, bioactive and aroma compounds. Türk Bilimsel Derlemeler Dergisi. 2019;12(1):26-34.
- Tagnamas Z, Kouhila M, Bahammou Y, Lamsyehe H, Moussaoui H, Idlimam A, Lamharrar A. Drying kinetics and energy analysis of carob seeds (Ceratonia siliqua L.) convective solar drying. Journal of Thermal Analysis and Calorimetry. 2021;147(3):2281-2291. doi: 10.1007/s10973-021-10632-6
- Othmen KB, Elfalleh W, Beltrán JMG, Esteban MÁ, Haddad M. An in vitro study of the effect of carob (Ceratonia siliqua L.) leaf extracts on gilthead seabream (Sparus aurata L.) leucocyte activities. Antioxidant, cytotoxic and bactericidal properties. Fish & Shellfish Immunology. 2020;99:35-43. doi: 10.1016/j.fsi.2020.02.005
- Rashed K. Phytochemical and biological effects of Ceratonia siliqua L.: a review. Journal of innovative pharmaceutical sciences and research. 2021;9(6):1-8. doi: 10.21276/IJIPSR.2021.09.06.900
- Aissa A, Chakroun I, Rejeb R, Ayed MH. Effect of partial dietary substitution of Carob (Ceratonia siliqua L.) to barley grains on diet digestibility in growing rabbits. Journal of New Sciences. 2021;79(1):4580-4585.
- Bazzato E, Lallai E, Serra E, Melis MT, Marignani M. Key role of small woodlots outside forest in a Mediterranean fragmented landscape. Forest Ecology and Management. 2021;496:119389. doi: 10.1016/j. foreco.2021.119389
- Jadrane I, Alfeddy MN, Dounas H, Kouisni L, Aziz F, Ouahmane L. Inoculation with selected indigenous mycorrhizal complex improves Ceratonia siliqua’s growth and response to drought stress. Saudi Journal of Biological Sciences. 2021;28(1):825-832. doi: 10.1016/j.sjbs.2020.11.018
- Paz-Kagan T, Chang JG, Shoshany M, Sternberg M, Karnieli A. Assessment of plant species distribution and diversity along a climatic gradient from Mediterranean woodlands to semi-arid shrublands. GIScience & Remote Sensing. 2021;58(6):929-953. doi: 10.1080/15481603.2021.1953770
- Sara N, Addi M, Abid M, Belkoura I. Effect of pre-sowing treatments and basal media on in vitro carob (Ceratonia siliqua L.) seed germination. Journal of Biotech Research. 2021;12:74-82.
- Yatim M, El Kahkahi R, Es-Sbata I, El-Askri T, ElOirdi S, Lakhlifi T, Belhaj A, Hafidi M, Zouhair R. Effects of pre-sowing treatments and abiotic stress on the germination of Ceratonia siliqua seeds of four Moroccan biomes. Annual Research & Review in Biology. 2020;35(12):11-31. doi: 10.9734/ARRB/2020/v35i1230307
- Pérez-García F. Germination characteristics and intrapopulation variation in carob (Ceratonia siliqua L.) seeds. Spanish Journal of Agricultural Research. 2020;7(2):398-406. doi: 10.5424/sjar/2009072-431
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