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Effect of biofertilizers and osmotic stress on morphological parameters of spring barley seedlings
- Authors: Panfilov A.L.1, Abdrashitov R.R.1
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Affiliations:
- Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
- Issue: Vol 17, No 4 (2022)
- Pages: 425-436
- Section: Morphology and biochemistry of plants
- URL: https://agrojournal.rudn.ru/agronomy/article/view/19825
- DOI: https://doi.org/10.22363/2312-797X-2022-17-4-425-436
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Abstract
One of ways to increase resistance of agricultural plants to abiotic stresses is the use of biofertilizers with anti-stress and growth-regulating properties. They improve absorption of nitrogen and phosphorus from organic fertilizers and soil. The purpose of the research was to study sowing qualities of seeds, morphological indicators of spring barley seedlings after presowing seed treatment with biofertilizers under conditions of sufficient humidification and osmotic stress. The laboratory experiment was carried out to study the effect of biofertilizers on seedlings of spring barley ( Hordeum vulgare L.) cv. Gubernatorsky under conditions of sufficient and insufficient humidification. The barley seeds were treated once according to the following scheme: 1. control (distilled water) 10 L/t; 2. Gumi 20 M potash (0.4 L/t); 3. Borogum-M complex (0.2 L/t); 4. PEG 6000 (100 g/L); 5. Gumi 20 M (0.4 L/t) + PEG 6000 (100 g/L); 6. Borogum-M complex (0.2 L/t) + PEG 6000 (100 g/L). Treatment of spring barley seeds with biofertilizers increased the germination rate and germination capacity by 3…5 and 2 %, respectively. When modeling drought using PEG-6000, the sowing qualities of seeds decreased by 4 %. Under conditions of sufficient humidification, the studied biofertilizers had a complex positive effect on shoot and root weight of barley seedlings. In addition, the average length of roots, shoots and the total root length increased. The number of roots did not change significantly. Lack of moisture decreased the effectiveness of Borogum-M complex biofertilizer, while Gumi 20 M potassium biofertilizer had a positive effect on the parameters of barley seedlings under water stress conditions.
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Fig. 1. Effect of biofertilizers and polyethylene glycol on spring barley seeds
Fig. 2. Effect of biofertilizers and PEG 6000 on the development of spring barley seedlings
Table 1. Effect of seed treatment with biofertilizers on the development of roots of spring barley seedlings under different humidification conditions
Variants | Average number of roots per seedling | Average root length | Total root length per seedling | |||
roots | ± to the control | cm | ± to the control | cm | ± to the control | |
Control | 4.8 | — | 5.4 | — | 26,3 | — |
Gumi 20 M potash | 4.9 | +0.1 | 6.6 | +1,2 | 32,0 | +5,7 |
Borogum-M complex | 4.9 | +0.1 | 6.1 | +0,7 | 29,6 | +3,3 |
PEG 6000 | 4.7 | –0.1 | 5.4 | 0,0 | 25,2 | –1,1 |
Gumi 20 M potash + PEG 6000 | 4.7 | –0.1 | 5.8 | +0,4 | 27,1 | +0,8 |
Borogum-M complex + PEG 6000 | 4.6 | –0.2 | 5.3 | –0,1 | 24,5 | –1,8 |
LSD05 | 0.2 | 0.6 | 3.5 | |||
Table 2. Factors determining the total length of germinal roots of barley under different humidification conditions,%
Factors | Optimal humidification | Insufficient humidification (PEG 6000) | ||||
Control | Gumi 20 M potash | Borogum-M complex |
Control | Gumi 20 M potash | Borogum-M complex | |
Number of roots | 25.1 | 45.8 | 39.5 | 20.6 | 41.8 | 52.7 |
Average root length | 74.1 | 53.3 | 59.8 | 75.1 | 57.6 | 46.5 |
Total | 99.2 | 99.1 | 99.3 | 95.7 | 99.4 | 99.2 |
Table 3. The effect of seed treatment with micro fertilizers on the length of spring sprouts barley in different humidification modes
Experience options | Average length of 1 sprout | |
cm | ± k control | |
Control | 5,0 | — |
Gumi 20 M potash | 5,5 | +0,5 |
Borogum-M complex | 5,1 | +0,1 |
PEG 6000 | 3,9 | –1,1 |
Gumi 20 M potash + PEG 6000 | 4,1 | –0,9 |
Borogum-M complex + PEG 6000 | 3,8 | –1,2 |
NSR05 = 0,4 | ||
About the authors
Alexander L. Panfilov
Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Author for correspondence.
Email: panfilov-1@mail.ru
ORCID iD: 0000-0002-1210-6350
Candidate of Agricultural Sciences, Leading Researcher, Department of Grain and Forage Crops Technologies
27/1 Gagarina ave., 460051, Orenburg, Russian FederationRinat R. Abdrashitov
Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences
Email: orniish_tzk@mail.ru
ORCID iD: 0000-0003-0946-068X
Candidate of Agricultural Sciences, Researcher, Department of Grain and Forage Crops Technologies
27/1 Gagarina ave., 460051, Orenburg, Russian FederationReferences
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