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Breeding characteristics of Romanov sheep depending on polymorphism of growth hormone gene
- Authors: Abramova M.V.1, Ilina A.V.1, Barysheva M.S.1, Malina J.I.1, Evdokimov E.G.1
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Affiliations:
- Federal Williams Research Center of Forage Production and Agroecology
- Issue: Vol 17, No 4 (2022)
- Pages: 514-526
- Section: Genetics and selection of animals
- URL: https://agrojournal.rudn.ru/agronomy/article/view/19833
- DOI: https://doi.org/10.22363/2312-797X-2022-17-4-514-526
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Abstract
The search for new markers of high productivity of sheep and the introduction of evaluation and selection methods for these indicators is an urgent direction in breeding of Russian sheep. The material for the research was the results of assessment of exterior, live weight of Romanov sheep. Genetic studies were carried out using tissue samples (ear plucking). Polymorphism of growth hormone gene in Romanov sheep was evaluated by restriction fragment length polymorphism analysis and polymerase chain reaction. Evaluation of polymorphism of growth hormone gene in the population of Romanov sheep showed the presence of three allelic variants - AA, AB and BB, with a frequency of 0.24, 0.63 and 0.14, respectively. The statistically significant difference between the indicators of live weight in animals with genotypes GHAB and GHAA, GHAB and GHBB was: at birth 210 g and 140 g (P ≥ 0.95), respectively; at weaning 350 g and 1260 g (P ≥ 0.95), respectively. The phenotypic variability of live weight was the highest at birth and at weaning and amounted to 31.7…32.5 % for all genotypes. Animals with genotypes GHAA and GHAB outperformed their peers with genotype GHBB in dimensions of rump width in tuber coxae, rump width in tuber trochanterica, chest width, chest circumference, and therefore, had a wide body. When studying the phenotypic and genetic correlations of measurements of exterior and live weight at the age of 12 months, a reliable positive relationship was established with height at withers height (rP = +0.13*; rG = +0.02), rump height (rP = +0.14**; rG = +0.03), rump length (rP = +0.14**; rG = +0.02). The new knowledge gained about polymorphism of GH gene and its relationship with productive traits of Romanov sheep will allow for in-depth evaluation, targeted selection and selection of individuals with desirable genotypes.
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Fig. 1. Frequency of occurrence of genotypes of the growth hormone (GH) gene in the population of Romanov sheep
Table 1. Indicators of live weight of sheep depending on genotype of growth hormone gene
Genotype | Body weight, kg (M±m) | |||
At birth | At weaning | At age of 300 days | At age of 365 days | |
AA | 2.07±0.06 | 19.03±0.42 | 38.52±0.51 | 43.03±0.52 |
AB | 2.28±0.04* | 19.38±0.24* | 38.64±0.29 | 42.98±0.30 |
BB | 2.14±0.09 | 18.12±0.58 | 37.97±0.82 | 42.26±0.82 |
Average value | 2.26±0.03 | 18.98±0.16 | 38.42±0.20 | 42.99±0.21 |
Note: *P≥0.95.
Fig. 2. Variability of the live weight of Romanov sheep
Table 2. Indicators of exterior of Romanov sheep (M±m)
Indicators of exterior, cm | Genotype of GH | Sample average | ||
АА | АВ | ВВ | ||
Withers height | 65.9±0.29 | 65.3±0.20 | 65.7±0.51 | 65.2±0.14 |
Rump height | 64.9±0.29 | 64.4±0.19 | 65.2±0.54 | 64.4±0.13 |
Body length | 68.2±0.31 | 67.8±0.21 | 68.1±0.55 | 67.6±0.15 |
Chest width | 28.2±0.351 | 27.5±0.20 | 26.9±0.42 | 27.4±0.15 |
Chest circumference | 94.6±0.96 | 93.8±0.43 | 92.2±1.05 | 92.9±0.32 |
Rump length | 23.1±0.18 | 23.0±0.10 | 22.8±0.25 | 22.9±0.07 |
Rump width in tuber coxae | 20.6±0.24** | 20.1±0.11* | 19.7±0.17 | 19.9±0.09 |
Rump width in tuber trochanterica | 19.1±0.26 | 18.9±0.13 | 18.9±0.30 | 18.8±0.09 |
Rump width | 12.6±0.19* | 12.8±0.13 | 14.0±0.52* | 12.7±0.10 |
Significant difference within one gene at *P≥0.95; **P≥0.99. Significant difference with the population average at 1P≥0.95.
Table 3. Correlation of indicators of exterior and body weight of Romanov sheep
Indicators of exterior, cm | Body weight at the age of 365 days, kg | |
Phenotypic correlation | Genetic correlation | |
Withers height | 0,13* | 0,02 |
Rump height | 0,14** | 0,03 |
Body length | 0,07 | 0,01 |
Chest width | –0,15** | –0,02 |
Chest circumference | –0,07 | –0,01 |
Rump length | 0,14** | 0,02 |
Rump width in tuber coxae | –0,09 | –0,02 |
Rump width in tuber trochanterica | –0,05 | –0,01 |
Rump width | –0,09 | –0,01 |
Significant difference at *P≥0.95; **P≥0.99.
About the authors
Marina V. Abramova
Federal Williams Research Center of Forage Production and Agroecology
Author for correspondence.
Email: abramovam2016@yandex.ru
ORCID iD: 0000-0003-3085-8844
Candidate of Agricultural Sciences, Leading Researcher, Laboratory of Selection and Breeding of Farm Animals, Yaroslavl Scientific Research Institute of livestock breeding and forage production - B ranch of Federal Williams Research Center of Forage Production and Agroecology
1 Lenina st., Mikhailovsky vil., Yaroslavl district, Yaroslavl region, 1150517, Russian FederationAnna V. Ilina
Federal Williams Research Center of Forage Production and Agroecology
Email: annabilina@yandex.ru
ORCID iD: 0000-0002-5046-1812
Candidate of Agricultural Sciences, Leading Researcher, Laboratory of Genetics and Biotechnology, Yaroslavl Scientific Research Institute of livestock breeding and forage production - B ranch of Federal Williams Research Center of Forage Production and Agroecology
1 Lenina st., Mikhailovsky vil., Yaroslavl district, Yaroslavl region, 1150517, Russian FederationMaria S. Barysheva
Federal Williams Research Center of Forage Production and Agroecology
Email: marija.baryshewa@yandex.ru
ORCID iD: 0000-0002-8140-4976
Senior Researcher, Laboratory for Selection and Breeding of Farm Animals, Yaroslavl Scientific Research Institute of livestock breeding and forage production - B ranch of Federal Williams Research Center of Forage Production and Agroecology
1 Lenina st., Mikhailovsky vil., Yaroslavl district, Yaroslavl region, 1150517, Russian FederationJulia I. Malina
Federal Williams Research Center of Forage Production and Agroecology
Email: julia.ibiw@gmail.com
ORCID iD: 0000-0003-1851-3683
Senior Researcher, Laboratory of Genetics and Biotechnology, Yaroslavl Scientific Research Institute of livestock breeding and forage production - Branch of Federal Williams Research Center of Forage Production and Agroecology
1 Lenina st., Mikhailovsky vil., Yaroslavl district, Yaroslavl region, 1150517, Russian FederationEvgeniy G. Evdokimov
Federal Williams Research Center of Forage Production and Agroecology
Email: skrad200052@yandex.ru
ORCID iD: 0000-0002-4265-1173
Researcher, Laboratory of Genetics and Biotechnology, Yaroslavl Scientific Research Institute of livestock breeding and forage production - Branch of Federal Williams Research Center of Forage Production and Agroecology
1 Lenina st., Mikhailovsky vil., Yaroslavl district, Yaroslavl region, 1150517, Russian FederationReferences
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