Genotypic clustering of 51 soybean cultivars and wild forms using SSR-markers

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Abstract

Soybean cultivars are characterized mainly by morphological and biochemical traits. However, researchers encounter difficulties when trying to use these parameters in cultivar identification and differentiation, making it difficult to work with closely related cultivar lines. Microsatellite markers or SSRs (simple sequence repeats) are an excellent tool for variety identification and differentiation, revealing the degree of genetic relatedness and copyright protection. The aim of the research was to obtain molecular genetic formulas for cultivated and wild soybean varieties with subsequent identification of their genetic relatedness. The object of the study was 51 samples of soybean (39 cultivars and 12 wild forms). Genomic DNA was isolated from the studied samples and then amplified. The obtained amplicons were separated in 2% agarose gel and the length of the fragments was detected in two replications. Nine microsatellite loci (Satt1, Satt2, Satt5, Satt9, Soyhsp176, Satt681, Sat_263, Satt141, Satt181) were used for molecular genetic characterization. Results demonstrating the length of each locus were analyzed by the UPGMA algorithm to record genetic relatedness or remoteness. The molecular genetic formulae of the studied samples were obtained, which can be further used to compile genetic passports. Based on the UPGMA algorithm, 51 soybean genotypes were grouped into 13 main clusters. Most of the soybean wild forms growing in the Amur Region demonstrated genetic proximity due to belonging to three closely located clusters. However, the soybean wild form from the Khabarovsk Territory (Dikaya soya 31) and one of the forms from the Amur Region (KZ-6337) were genetically distant from other groups of soybean wild forms. These results indicate the adequacy of the use of 9 SSR locuses for identification tasks, identification of relatedness and further passportization of soybeans.

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About the authors

Alena A. Ivaniy

Russian Research Institute of Soybean

Author for correspondence.
Email: iaa@vniisoi.ru
ORCID iD: 0009-0004-7304-7771

Junior Researcher, Laboratory of Biotechnology

19 Ignatievskoye Shosse, Blagoveshchensk, Amur region, 675027, Russian Federation

Andrey A. Penzin

Russian Research Institute of Soybean

Email: paa@vniisoi.ru
ORCID iD: 0000-0002-8578-9818
SPIN-code: 1467-9500

Research Associate, Laboratory of Biotechnology

19 Ignatievskoye Shosse, Blagoveshchensk, Amur region, 675027, Russian Federation

Olga N. Bondarenko

Russian Research Institute of Soybean

Email: ton@vniisoi.ru
ORCID iD: 0000-0002-5051-7695
SPIN-code: 1592-0588

Research Associate, Laboratory of Biotechnology

19 Ignatievskoye Shosse, Blagoveshchensk, Amur region, 675027, Russian Federation

Anastasia A. Blinova

Russian Research Institute of Soybean

Email: baa@vniisoi.ru
ORCID iD: 0000-0002-7234-0595
SPIN-code: 8575-0595

Researcher, Head of the biotechnology laboratory

19 Ignatievskoye Shosse, Blagoveshchensk, Amur region, 675027, Russian Federation

Alina E. Gretchenko

Russian Research Institute of Soybean

Email: gae@vniisoi.ru
ORCID iD: 0000-0003-3930-5672
SPIN-code: 3750-4348

Researcher, Laboratory of plant physiology and biochemistry

19 Ignatievskoye Shosse, Blagoveshchensk, Amur region, 675027, Russian Federation

Lyubov E. Ivachenko

Russian Research Institute of Soybean

Email: ivachenko-rog@yandex.ru
ORCID iD: 0000-0003-4870-2223
SPIN-code: 4641-4820

Doctor of Biological Sciences, Leading Researcher, Laboratory of Biotechnology

19 Ignatievskoye Shosse, Blagoveshchensk, Amur region, 675027, Russian Federation

Pavel D. Timkin

Russian Research Institute of Soybean

Email: tpd@vniisoi.ru
ORCID iD: 0000-0001-6655-1049
SPIN-code: 2729-2815

Junior Researcher, Laboratory of Biotechnology

19 Ignatievskoye Shosse, Blagoveshchensk, Amur region, 675027, Russian Federation

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Copyright (c) 2025 Ivaniy A.A., Penzin A.A., Bondarenko O.N., Blinova A.A., Gretchenko A.E., Ivachenko L.E., Timkin P.D.

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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