Immunological parameters in serous catarrhal inflammation of the udder in lactating cows

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Abstract

The aim of this study is to investigate the immunological parameters of blood in lactating cows with serous-catarrhal mastitis to identify the pathogenetic mechanisms of the disease. The research was carried out in autumn in one of the livestock farms of the Amur region. The object of the study was lactating cows of Holstein breed at 2-4 months of lactation. Groups of cows were formed by 10 animals in each: control (healthy) and experimental (sick with serous catarrhal mastitis). The inflammatory process in the mammary gland of lactating cows was caused by tissue damage and complicated by opportunistic microflora. Protein metabolism disorders (hypoalbuminemia, dysproteinemia) and signs of haemoconcentration (increased haematocrit and haemoglobin, erythrocytosis) were observed in sick animals. Conditionally pathogenic microflora and products of inflammation in the mammary gland stimulated cellular and humoral immunity.

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Microbiological profile of milk from affected udder lobes in lactating cows,%
Source: compiled by M.E. Ostyakova, K.S. Kositsyna, V.K. Irkhina.

Table 1
Biochemical analysis of blood of lactating cows  in serous catarrhal mastitis M ± m, n = 10

 Parameter

 Cow groups

 Control

 Experimental

 Total protein, g/l

 79.5 ± 1.14

 86.2 ± 2.13*

 Albumins,%

 41.9 ± 0.58

 21,0 ± 1.82***

 a-globulins,%

 12.3 ± 0.77

 13.2 ± 1.97

 β-globulins,%

 17.6 ± 0.87

 40.3 ± 3.06***

 γ-globulins,%

 28.2 ± 1.21

 25.6 ± 2.96

Note. * — p < 0.05; ***— p < 0.001, in relation to the control group.
Source: compiled by M.E. Ostyakova, K.S. Kositsyna, V.K. Irkhina.

Table 2
Immunologic indicators of blood of lactating cows  in serous catarrhal mastitis M ± m, n = 10

 Parameter

 Cow groups

 Control

 Experimental

 Erythrocytes, 1012 l

 5.7 ± 0.13

 7.7 ± 0.21***

 Leukocytes, 109 l

 6.5 ± 0.25

 9.4 ± 0.44***

 Hemoglobin, g/l

 113.4 ± 3.13

 127.0 ± 2.39**

 Hematocrit, %

 66.5 ± 1.75

 88.9 ± 1.42***

 Color index

 0.9 ± 0.03

 1.1 ± 0.02

 Eosinophils, %

 2.3 ± 0.54

 1.9 ± 0.66

 Band neutrophils, %

 1.3 ± 0.15

 3.1 ± 0.50**

 Segmented neutrophils, %

 31.5 ± 1.05

 39.3 ± 4.01

 Lymphocytes, %

 63.7 ± 1.18

 52.8 ± 4.50*

 Monocytes, %

 1.2 ± 0.39

 2.9 ± 0.91

 FAN, %

 88.3 ± 0.78

 97.7 ± 1.46***

 LASK, %

 15.3 ± 0.84

 19.0 ± 1.51*

 CIC, g/l

 36.6 ± 1.36

 90.5 ± 5.93***

Note. * — p < 0.05; ** — p < 0.01, *** — p < 0.001, in relation to the control group; basophils, myelocytes and metamyelocytes were not detected.
Source: compiled by M.E. Ostyakova, K.S. Kositsyna, V.K. Irkhina.

×

About the authors

Marina E. Ostyakova

Far East Zone Research Veterinary Institute

Email: dalznividv@mail.ru
ORCID iD: 0000-0002-2996-0991
SPIN-code: 3038-0685

Doctor of Biological Sciences, Associate Professor, Director

112 Severnaya St., Blagoveshchensk, Amur Region, 675005, Russian Federation

Ksenia S. Kositsyna

Far East Zone Research Veterinary Institute; Far Eastern State Agrarian University

Author for correspondence.
Email: kseniya-kos1997@yandex.ru
ORCID iD: 0009-0005-6247-0280
SPIN-code: 9056-4692

Junior Researcher, Department of Microbiology, Virology and Immunology, Far Eastern Zone Research Veterinary Institute; Assistant of the Department of Veterinary and Sanitary Expertise, Epizootology and Microbiology, Far Eastern State Agrarian University

112 Severnaya St., Blagoveshchensk, Amur Region, 675005, Russian Federation; 86 Politekhnicheskaya St., Blagoveshchensk, Amur Region, 675005, Russian Federation

Vera K. Irkhin

Far East Zone Research Veterinary Institute

Email: dalznividv@mail.ru
ORCID iD: 0000-0003-4553-7189
SPIN-code: 7893-0109

Researcher of the Department of Animal Husbandry and Poultry Farming

112 Severnaya St., Blagoveshchensk, Amur Region, 675005, Russian Federation

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1. Microbiological profile of milk from affected udder lobes in lactating cows,%
Source: compiled by M.E. Ostyakova, K.S. Kositsyna, V.K. Irkhina.

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