Effect of the combined use of a probiotic and an organic acid complex on enteromicrobiocenosis, hematological and immunological parametersof quails with experimental dysbiosis

Dysbiosis in poultry is a common syndrome that is accompanied by decreased productivity and compromised animal health. Despite the availability of various drugs for correcting the microbiota, the problem of treating dysbiosis remains relevant due to the poor engraftment of probiotic bacteria and their limited antagonistic activity. A promising approach is the combined use of probiotics and organic acids, aimed at improving microbiocenosis and increasing poultry productivity. The aim of this study was to evaluate the effect of the combined use of the probiotic Sibersil® and the organic acid complex Salmocil FL on enteromicrobiocenosis, hematological and immunobiological parameters of quails with experimental dysbiosis. For the experiment, 4 groups of quails were formed: group I was kept on a basal diet; group II, with experimentally induced dysbiosis, was also kept on a basal diet; group III consisted of birds with experimental dysbiosis, which were supplemented with the probiotic Sibersil® in addition to the basal diet; group IV consisted of quails with experimental dysbiosis, which were supplemented with the probiotic Sibersil® and the organic acid complex Salmocil FL. The isolation and identification of bacteria were carried out using standard bacteriological methods and MALDI-TOF mass spectrometry. Hematological and immunological blood parameters were determined in birds of all experimental groups. Bacteriological studies revealed that, compared with group II (dysbiosis control), quails in groups III and IV showed an increase in the abundance of Bifidobacterium spp. and a decrease in Enterobacter spp. and Streptococcus spp. In quails of groups I and II, the presence of lactose-positive, lactose-negative, and hemolytic E. coli cultures was noted in the luminal microflora. Hematological and immunological studies showed that in birds of group II (dysbiosis), there was a decrease in T-lymphocyte count to 1.04 ± 0.12 thousand/µL, whereas in groups III and IV, their counts increased to 1.50 ± 0.10 and 1.68 ± 0.20 thousand/µL, respectively. Thus, as a result of the conducted research, it was found that the combined use of the probiotic Sibersil® and the organic acid complex Salmocil FL contributed to the normalization of the microbiota in various biotopes of the quail intestinal tract and improved the immunological parameters of the birds.