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Features of the functional state of the organism of sheep under stress
- Authors: Yuldashbaev Y.A.1, Vatnikov Y.A.2, Rudenko P.A.2,3, Rudenko A.A.2,4
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Affiliations:
- Russian State Agrarian University - Moscow Timiryazev Agricultural Academy
- RUDN University
- Branch of Shemyakin - Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Moscow State University of Food Production
- Issue: Vol 17, No 2 (2022)
- Pages: 193-202
- Section: Animal breeding
- URL: https://agrojournal.rudn.ru/agronomy/article/view/19765
- DOI: https://doi.org/10.22363/2312-797X-2022-17-2-193-202
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Abstract
The stresses that arise during routine animal husbandry procedures, such as milking, shearing, weighing, loading and hoof care, are an actual problem in animal husbandry. The components of economic damage under stress are a decrease in overall resistance and productivity, deterioration in product quality, an increase in labor costs and funds per unit of production, an increase in animal morbidity and mortality. The studies were carried out on the basis of ‘Belozernoe’ farm in the Salsky district, the Rostov region. A complex of clinical and laboratory studies was performed on 18 sheep (10 Romanov and 8 Tsigay breeds) aged 2 to 3 years, before and after a planned routine shearing. Stress in sheep caused by planned shearing was clinically manifested by general depression, in some cases, subfebrile fever, moderate tachypnea and tachycardia. It was shown that stress was accompanied by anemia, the development of erythropenia and leukopenia. Immunocompetent cells in the blood of experimental animals during stress development were analyzed. Severe lymphocytopenia was found to occur after a haircut. In addition, in the blood of experimental animals under stress, a tendency to a decrease in T-total cells was noted, which was accompanied by an increase in 0-cells, which was significant in sheep of the Tsigay breed (p<0.05). When analyzing the main immunoregulatory T cells in sheep under stress, a significant increase in T-suppressors was revealed with a relatively stable level of T-helpers in the blood studied. Thus, a significant (p<0.05) increase in the level of T-suppressors in sheep of Romanov and Tsigay breeds was recorded; it was by 1.15 and 1.19 times higher compared with the initial data. It should be noted that we did not find any significant differences between animals of Romanov and Tsigay breeds in stress development after shearing. The obtained data should be taken into account in the further search for methods of stress correction in sheep.
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Table 1. Basic physiological parameters of sheep before and after shearing
Indicators | Breed | n | Sheep | |
Before haircut | After haircut | |||
Body temperature, °C | І | 10 | 39.57±0.26 | 39.75±0.22 |
ІІ | 8 | 39.65±0.17 | 40.04±0.14 | |
HR, min | І | 10 | 77.42±1.91 | 84.77±1.73* |
ІІ | 8 | 80.51±1.03 | 85.52±1.23** | |
RR, min | І | 10 | 40.71±1.42 | 46.77±1.90* |
ІІ | 8 | 40.87±1.31 | 44.87±1.24* | |
Note: HR — heart rate; RR — r espiratory rate; I — Romanov breed; II — T sigay breed; n — number of animals in the group. * — р < 0,05; ** — р < 0,01; *** — р < 0,001.
Table 2. Effect of shearing on hematological parameters of sheep
Indicators | Breed | n | Sheep | |
Before haircut | After haircut | |||
Hemoglobin, g/l | І | 10 | 112.25±1.12 | 97.23±1.01*** |
ІІ | 8 | 109.33±1.04 | 99.67±1.25*** | |
Red blood cells, Т/l | І | 10 | 4.26±0.12 | 3.77±0.09** |
ІІ | 8 | 4.41±0.14 | 3.82±0.16* | |
CI, conv. units | І | 10 | 0.89±0.07 | 0.73±0.09 |
ІІ | 8 | 0.85±0.04 | 0.69±0.08 | |
Leukocytes, G/l | І | 10 | 7.75±0.16 | 6.87±0.19** |
ІІ | 8 | 7.61±0.26 | 6.53±0.23** | |
Eosinophils,% | І | 10 | 1.59±0.17 | 1.92±0.11 |
ІІ | 8 | 1.47±0.11 | 1.88±0.18 | |
Lymphocytes,% | І | 10 | 46.82±1.32 | 41.64±1.33* |
ІІ | 8 | 45.64±1.27 | 40.87±1.21* | |
T- total,% | І | 10 | 53.37±1.16 | 50.84±1.28 |
ІІ | 8 | 52.66±1.34 | 49.16±1.48 | |
T-helpers,% | І | 10 | 36.42±1.27 | 36.59±1.41 |
ІІ | 8 | 36.17±1.22 | 36.47±1.34 | |
T-suppressors,% | І | 10 | 14.59±0.73 | 16.85±0.67* |
ІІ | 8 | 14.49±0.64 | 17.23±0.76* | |
IRI | І | 10 | 2.49±0.37 | 2.17±0.28 |
ІІ | 8 | 2.50±0.24 | 2.12±0.37 | |
0-cells,% | І | 10 | 24.56±1.18 | 27.64±1.22 |
ІІ | 8 | 25.67±1.14 | 30.07±1.17* | |
B- total,% | І | 10 | 22.07±1.19 | 21.52±1.16 |
ІІ | 8 | 21.67±1.23 | 20.84±1.13 | |
Note: CI — color indicator; IRI — immunoregulatory index; I — Romanov breed; II — T sigay breed; n — number of animals in the group; * — р < 0,05; ** — р < 0,01; *** — р < 0,001.
About the authors
Yusupzhan A. Yuldashbaev
Russian State Agrarian University - Moscow Timiryazev Agricultural Academy
Email: zoo@rgau-msha.ru
ORCID iD: 0000-0002-7150-1131
RAS Academician (Full Member), Doctor of Agricultural Sciences, Professor, Director of Institute of Zootechnics and Biology, Professor, Department of Private Animal Science
49 Timiryazevskaya st., Moscow, 127550, Russian FederationYury A. Vatnikov
RUDN University
Email: vatnikov@yandex.ru
ORCID iD: 0000-0003-0036-3402
Doctor of Veterinary Sciences, Professor, Director of Veterinary Medicine Department, Agrarian and Technological Institute
6 Miklukho-Maklaya st., Moscow, 117198, Russian FederationPavel A. Rudenko
RUDN University; Branch of Shemyakin - Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: pavelrudenko76@yandex.ru
ORCID iD: 0000-0002-0418-9918
Doctor of Veterinary Sciences, Leading researcher, Biological Testing Laboratory, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences ; Associate Professor, Department of Veterinary Medicine, Agrarian and Technological Institute, Peoples’ Friendship University of Russia (RUDN University)
6 Miklukho-Maklaya st., Moscow, 117198, Russian Federation; 6 Nauki av., Pushchino, Moscow region, 142290, Russian FederationAndrey A. Rudenko
RUDN University; Moscow State University of Food Production
Author for correspondence.
Email: vetrudek@yandex.ru
ORCID iD: 0000-0002-6434-3497
Doctor of Veterinary Sciences, Professor; Department of Veterinary Medicine, Moscow State University of Food Production; Associate Professor, Department of Veterinary Medicine, Agrarian and Technological Institute, Peoples’ Friendship University of Russia (RUDN University)
6 Miklukho-Maklaya st., Moscow, 117198, Russian Federation; 11 Volokolamskoye highway, Moscow, 125080, Russian FederationReferences
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