General patternsof Aspergillus spp. biofilm development dynamics

During the development of local and systemic infections of humans and animals, phytopathology, degradation of natural and synthetic polymers, residential and industrial structures, the adaptive potential of pathogens is inhibited by QS molecules that block the synthesis of intercellular communication inducers. The aim of the study was to analyze the dynamics of changes in morphofunctional parameters during the initiation, development, and dispersion of Aspergillus spp. fungi. To study the phenotypic characteristics and take into account the morphometric parameters of biofilms, microorganisms were cultivated at 35 ± 2.0°C for 168 ± 1h. Cultures of A. flavus, A. fumigatus, and A. niger microorganisms are fast-growing; depending on the composition of the nutrient media and the cultivation time, they differed both in the volume of aerial mycelium and in the staining of the reverse side. During the implementation of intercellular communication processes at the early stages of development, adhesion of conidia to the substrate surface was initiated. Due to the apical growth of germinal tubules, filiform structures - hyphae - subsequently differentiated. As cultivation time increased, areas of significant hyphal consolidation emerged, and conidiogenic structures differentiated. General patterns of heterogeneous biofilm development during the representation of QS signaling molecules are mediated by growth cyclicity and population adaptation to environmental factors. Understanding the mechanisms of initiation, persistence, and dispersion during adventitious sporulation and filamentation of micromycetes will facilitate the optimization of long-term retrospective mycological studies, the development of effective methods for biofilm eradication, and the rationalization of the use of fungal biosynthetic activity in biotechnology and bioremediation.