Indication and identification of infectious agents in animal products based on biochip technology with a biotin label and colorimetric detection

A V Lokorev; Локорев Андрей Владимирович; I M Nityaga; Нитяга Инга Михайловна; D V Fedyushin; Федюшин Дмитрий Владимирович; N G Khomenets; Хоменец Николай Геннадьевич; L A Shamanova; Шаманова Людмила Андреевна

doi:10.22363/2312-797X-2016-3-72-78

Indication and identification of infectious agents in animal products based on biochip technology with a biotin label and colorimetric detection

Authors: Lokorev AV¹, Nityaga IM², Fedyushin DV², Khomenets NG³, Shamanova LA¹
Affiliations:
1. FGBNU “All-Russian Research and TechnologyInstitute of Biological Industry”
2. FGBOU VPO “Moscow State University of Food Production”
3. 3Peoples’ Friendship University of Russia
Issue: No 3 (2016)
Pages: 72-78
Section: Articles
URL: https://agrojournal.rudn.ru/agronomy/article/view/13953
DOI: https://doi.org/10.22363/2312-797X-2016-3-72-78

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Abstract

The results of the development of DNA chips with biotin uterus and colorimetric detection to indicate and identify pathogens in animal products. The constant need to monitor for dangerous infectious diseases makes the urgency of developing new effective methods of identification of pathogens of these diseases. The high specificity and sensitivity of the technique, is 103-104 bacterial cells. The analysis takes 5-7 hours. Visual assessment of the end result allows for biochip test systems in stationary and field conditions, laboratories and regional reference centers. The application of modern biochip technology research quickly and with high reliability to detect infectious agents in a variety of biological materials, typified by strains in order to establish a common source of infection.

Keywords

chip, biochip, microarray technology, a biotin label, the identification of infectious agents

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Vol 19, No 2 (2024)

Indication and identification of infectious agents in animal products based on biochip technology with a biotin label and colorimetric detection

Full Text

Abstract

Keywords

About the authors

A V Lokorev

I M Nityaga

D V Fedyushin

N G Khomenets

L A Shamanova

References

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