Controlling the reliability of results received during laboratory testing for phytosanitary purposes when molecular diagnostic methods are used

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Abstract

An internal PCR control system based on a mouse gene fragment cloned into a vector was developed. The created internal control system allowed for choosing a method for DNA extraction from bio-baits which doesn't inhibit the amplification process when Phytophthora sp. is being diagnosed. During the process of diagnosing the pathogen of the ring rot of potato, it was shown that the developed internal control in real-time PCR turned out to be more informative than the mitochondrial gene of a host plant used for that purpose.

About the authors

E S Mazurin

Peoples' Friendship University of Russia

Email: zarauh@mail.ru
Кафедра генетики, растениеводства и защиты растенийФГБУ «Всероссийский центр карантина растений»ул. Пограничная, 32, пос. Быково, Раменский р-н,Московская обл,. Россия, 140150; Российский университет дружбы народов; Peoples' Friendship University of Russia

M B Kopina

All-Russian Plant Quarantine Center

Email: marija508a@mail.ru
All-Russian Plant Quarantine Center

N A Sherokolava

All-Russian Plant Quarantine Center

Email: natalia_sh@mail.ru
All-Russian Plant Quarantine Center

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Copyright (c) 2012 Мазурин Е.С., Копина М.Б., Шероколава Н.А.

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