Specificity of combinations of qualitative and quantitative characteristics of glucovine in genotypes of allocytoplasmatic spruce wheat with allel of wild type Wx-B1a
- Authors: Semenov OG1, Divashur MG2,3, Haitembu G.S.4, Plushikov VG1, Hupacaria TI2, Vvedenskiy VV1, Pochtovyy AA5
-
Affiliations:
- Рeoples’ Friendship University of Russia (RUDN University)
- Russian State Agrarian University
- All-Russia Research Institute of Agricultural Biotechnology
- Ministry of Agriculture, Water and Forestry
- Lomonosov Moscow State University
- Issue: Vol 13, No 1 (2018)
- Pages: 14-25
- Section: Crop production
- URL: https://agrojournal.rudn.ru/agronomy/article/view/18079
- DOI: https://doi.org/10.22363/2312-797X-2018-13-1-14-25
Cite item
Full Text
Abstract
As a result of screening of the allelic composition of genes associated with baking properties, a significant genotypic variety of forms of allocytoplasmic spring wheat (ATSGG) from the ATI PFUR collection was established. In addition to the altered forms, 15 genotypes were isolated as a result of recombinations and introgression, in the genome of which the presence of a allel of wild type Wx-B1a (primer 4F / 4R) was detected. Analysis of the content and quality of gluten in these forms of ACPG made it possible to differentiate these genotypes according to their functional characteristics, which are related to baking properties. The amplitude of the differences in the genotypes of the ACPG in terms of the mass fraction of gluten is from 21.7% to 37.8%, the quality of gluten according to the IDK parameters in the majority of the studied genotypes of the I-st group. The genotypes of the category of strong wheat are of particular value: No. 24 (cytoplasm T. timopheevii), in which the mass fraction of gluten is 37.8% (class of super-strong wheat), and also genotypes of the 1st class, in which the gluten content is not less than 32%, and the quality of gluten is not lower than the I-st group (IDK - 43-77 units.). These are genotypes No. 25 (the cytoplasm of T. timopheevii) and No. 29 (the cytoplasm of T.aestivum L., as a result of backcrossing). To the category of strong wheat of the 2nd class (mass fraction of gluten is not lower than 28%, and the quality of gluten is the I-st group), four genotypes are classified. The category of valuable wheat of the 3rd class includes two genotypes, in which the mass fraction of gluten is not less than 25%. However, the quality of gluten in these genotypes is not II-th group, but higher - it corresponds to the I-st group. Genotypes with a specific combination of the mass fraction of gluten characteristic of strong and valuable wheat, with the qualitative characteristics of gluten of the I-th group, expand the range of their intended use in the production of bakery products.
About the authors
O G Semenov
Рeoples’ Friendship University of Russia (RUDN University)
Author for correspondence.
Email: semenov_og@rudn.university
кандидат биологических наук, профессор департамента техносферной безопасности Аграрно-технологического института Российского университета дружбы народов
Miklukho-Maklaya st., 6, Moscow, Russia, 117198M G Divashur
Russian State Agrarian University; All-Russia Research Institute of Agricultural Biotechnology
Email: genetics@timacad.ru
кандидат биологических наук, доцент Центра молекулярной биотехнологии РГАУ-МСХА им. К.А. Тимирязева
Timiryazevskaya st., 49, Moscow, Russia, 127550; Timiryazevskaya st., 42, Moscow, 127550, RussiaGerhard Shangeshapwako Haitembu
Ministry of Agriculture, Water and Forestry
Email: g.haitembu@yahoo.com
кандидат сельскохозяйственных наук, научный сотрудник лаборатории аналитических услуг и разработки продуктов (отдел биотехнологии) Министерства сельского, водного и лесного хозяйства. Виндхук. Намибия
Government Office Park, Luther st., Windhoek, NamibiaV G Plushikov
Рeoples’ Friendship University of Russia (RUDN University)
Email: plyuschikov_vg@rudn.university
доктор сельскохозяйственных наук, профессор, директор Аграрно-технологического института Российского университета дружбы народов
Miklukho-Maklaya st., 6, Moscow, Russia, 117198T I Hupacaria
Russian State Agrarian University
Email: genetics@timacad.ru
кандидат биологических наук, профессор кафедры генетики, биотехнологии, селекции и семеноводства РГАУ-МСХА им. К.А. Тимирязева
Timiryazevskaya st., 49, Moscow, Russia, 127550V V Vvedenskiy
Рeoples’ Friendship University of Russia (RUDN University)
Email: vvedensky_vv@rudn.university
кандидат сельскохозяйственных наук, доцент, директор агробиотехнологического департамента Аграрно-технологического института Российского университета дружбы народов
Miklukho-Maklaya st., 6, Moscow, Russia, 117198A A Pochtovyy
Lomonosov Moscow State University
Email: genetics@timacad.ru
аспирант биологического факультета Московского государственного университета имени М.В. Ломоносова
GSP-1, Leninskie Gory, Moscow, Russia, 119991References
- Danilenko N.G. and Davydenko O.G. The worlds of organelle genomes. Minsk: Tekhnologiya, 2003.
- Meleshkina E.P., Kolomiec S.N. et al. Targeted use of grain and flour — Time Requiremen. Food Industry. 2013. Vol. 9. P. 64—66.
- Semenov O.G. Allo-cytoplasmic wheat. Biological bases of selection: monograph. M.: Publishing house of PFUR, 2000.
- Haitembu Gerhard Shangeshapwako. Realization of potential quality grains of various morphobiotypes of allocytoplasmic spring wheat in conditions of the Moscow region. Dissertation. Moscow. 2017.
- Horvat D., Jurcovic Z., Sudar R., Pavlinic D., Simic G. The relative amounts of HMW glutenin subunits of OS wheat cultivars in relation to bread-making quality. Cereal Res. 2002. Vol. 30. P. 415—422.
- Payne P.I. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Ann. Rev. Plant Physiol. 1987. V. 38. P. 141—153.
- Payne P.I., Nigthingale M.A., Krattiger A.F., and Holt L.M. The relationship between the composition and the breadmaking quality of British grown wheat varieties. J. Sci. Food Agric. 1987. Vol.40. P.51—65.
- Tanaka H., Toyoda S., Tsijmoto H. Diversity of Low-Molecular-Weight glutenin subunit genes in Asian common wheat (Triticum aestivum L.). Breeding Science, 2005.
- Weegels P.L., Hamer R.J., Schofield I.D. Functional properties of wheat glutenin. Journal of Cereal Science. 1996. Vol. 23. P. 1—18.
- Divashuk M.G., Klimushina M.V., Karlov G I. Molecular genetic characteristics of the WX-B1E allele from common wheat and applicability of the DNA markers for its identification. Russian Journal of Genetics. 2011. Т. 47. № 12. P. 1428—1432.
- Klimushina M.V., Gladkih N.I., Divashuk M.G., Bespalova L.A., Vasilyev A.V., Karlov G.I. Distribution of allelic variants of Wx genes in the common wheat collection made at the Krasnodar Lukyanenko research Institute of Agriculture. Vavilov journal of genetics and plant breeding. 2012. T. 16. No. 1. P. 187—192.
- Klimushina M.V., Kroupin P.Yu., Divashuk M.G., Karlov G.I. About optimization of molecular labeling of wheat Waxy-genes for mass-selestion. Agricultural biology. 2010. Vol. 5. P. 36—41.
- Klimushina M.V., Divashuk M.G., Karlov G.I., Mokhammed T.A.K., Semenov O.G Analysis of allelic state of genes responsible for baking properties in allocytoplasmic wheat hybrids. Russian Journal of Genetics. 2013. Т. 49. № 5. P. 530—538.
- Tawfeek A.K.M., Divashuk M.G., Semenov O.G. Screening of allelic genes, associated with the baking quality of grain in various hybrid allocytoplasmic sping wheat. Conference Papers of the VII International Scientific and Practical Conference of Professors, Young Scientists, Post-graduate and Under-Graduate students. Russian State Agrarian University. 2015. P. 160—161.