Microbiological nutrient media the new format in the veterinary-sanitary assessment food and raw materials of animal origin

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Abstract


The main task of veterinary-sanitary examination is to prevent infectious and parasitic diseases that spread among humans and animals through food, feed and industrial products of animal and vegetable origin. This is very important conduct microbiological raw material quality control, food production and disinfection measures carried out, aimed at the reorganization of the production environment. In this connection, development of methods acquires relevance rapid microbiological analysis to identify organisms of sanitary illustrative and other pathogens in a variety of materials. The aim of this work is the comparative study of the effectiveness of the practical use of classical bacteriological culture media and nutrient media of a new format in the veterinary and sanitary evaluation of food and animal feed. As part of this work the traditional media (agar Baird-Parker) and culture media of the new format - 3M™ Petrifilm™ Staph Express Count Plate (STX) were subjected to comparative study in terms of sensitivity and the time required to identify cells S. aureus . The procedures described in ISO 31746-2012 and MUK 42.2884-11 were taken as research algorithms. For the purity of the experiment using artificially contaminated with Staphylococcus aureus cells of the raw milk of cows. 3M™ Petrifilm™ Staph Express Count Plate (STX) may be used in the food industry and regulatory authorities in monitoring to obtain objective information on the contamination of raw materials and food products dangerous pathogens in a very short period of time. This in turn is necessary for the removal of low-quality products from the retail network and avoid poor-quality raw materials to produce products without heat obrabotku. Takzhe it should be noted that due to the simplicity of seeding procedure there is no need for qualified personnel at the stage of sowing and the initial evaluation results. Also achieved significant savings in time, consumables and media at the stage of the primary planting material using the 3M™ Petrifilm™.

About the authors

I Yu Egorova

SSI “All-Russian Scientific Research Institute of Veterinary Virology and Microbiology RAAS”

Author for correspondence.
Email: iegorova@list.ru
Academika Bakulova str., p. 1, Pos. Volginsky, Petushki district, Vladimir region, Russia, 601125

V E Nikitchenko

Peoples’ Friendship University of Russia (RUDN University)

Email: v.e.nikitchenko@mail.ru
Miklukho-Maklaya st., 6, Moscow, Russia, 117198

D V Nikitchenko

Peoples’ Friendship University of Russia (RUDN University)

Email: nikitchenko@mail.ru
Miklukho-Maklaya st., 6, Moscow, Russia, 117198

A N Chernysheva

Peoples’ Friendship University of Russia (RUDN University)

Email: v.e.nikitchenko@mail.ru
Miklukho-Maklaya st., 6, Moscow, Russia, 117198

E O Rystsova

Peoples’ Friendship University of Russia (RUDN University)

Email: v.e.nikitchenko@mail.ru
Miklukho-Maklaya st., 6, Moscow, Russia, 117198

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Copyright (c) 2017 Егорова И.Ю., Никитченко В.Е., Никитченко Д.В., Чернышева А.Н., Рысцова Е.О.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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