Contribution of consensus 5'-untranslated region to the translational efficiency of heterologous genes in plant cells

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Abstract


The results of bioinformatics analysis allowed to reveal the following consistent pattern: the average length of the 5'-untranslated region (5'-UTRs) for most A. thaliana's genes with high expression levels range from 80 to 120 bp, with an average GC content of 36,5%. Based on alignment results was defined motive in 5'-UTRs, as a new regulatory element, which could potentially provide highly efficient expression and synthesis of the target product in plants. This sequence has a length of 87 bp and GC content of 35.6%. It was demonstrated that consensus sequence of 5'-UTRs increased accumulation of the bi-reporter protein more than 25%, by that acting as a potential positive regulatory element on translational efficiency.

M G Quimisse

Peoples’ Friendship University of Russia

Author for correspondence.
Email: kimisse@rambler.ru
Department of botany, plant physiology and agrobiotechnology

K V Kabardaeva

RSAU - MTAA named after K.A. Timiryazev

Email: kabardaewa@yandex.ru

O A Gra

FSBIS K.A. Timiryazev institute of plant physiology RAS

Email: olgagra@gmail.com

A A Tyurin

FSBIS K.A. Timiryazev institute of plant physiology RAS

Email: alexjofar@gmail.com

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Copyright (c) 2015 Кимиссе М.Г., Кабардаева К.В., Гра О.А., Тюрин А.А.

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