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Selection combinations of primers for determining intervarietal and intravarietal polymorphism Glycine hispida
- Authors: Romanova EV1, Kochumova AA2, Shmelkova EO1
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Affiliations:
- Peoples’ Friendship University of Russia
- Vavilov Institute of General Genetics Russian Academy of Sciences
- Issue: No 2 (2015)
- Pages: 16-23
- Section: Articles
- URL: https://agrojournal.rudn.ru/agronomy/article/view/1348
- DOI: https://doi.org/10.22363/2312-797X-2015-2-16-23
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Abstract
The article describes the experience of selecting combinations of primers for AFLP-analysis intravarietal and intervarietal polymorphism soybean accessions. During the experiment, has been tested 18 primer combinations. As a result, were selected 5 primer combinations, the most informative labeling for the soybean genome. Total detect 132 AFLP-fragments, of which 60 were polymorphic and characterized by individual genotypes varieties.
About the authors
E V Romanova
Peoples’ Friendship University of Russia
Author for correspondence.
Email: evroma2008@yandex.ru
Department of genetic, plant growing and plant protection
A A Kochumova
Vavilov Institute of General Genetics Russian Academy of Sciences
Email: aichka89@mail.ru
Laboratory of Plant Genetics
E O Shmelkova
Peoples’ Friendship University of Russia
Email: shmelkoffa@gmail.com
Department of genetic, plant growing and plant protection
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